| Ascenta Software Ltd | Information & communication | 2024 |
| Aspire Personal and Organisational Development Limited | Health & social care | 2006 |
| Aspire Sports Coaching Academy Limited | Arts & recreation | 2008 |
| Aspire Therapy Services Limited | Health & social care | 2013 |
| Associated Roto-Plastics Limited | Professional & scientific | 1987 |
| Assured Hygiene Limited | Administrative & support | 2007 |
| Aston's Access Auto Locksmiths Ltd | Other services | 2013 |
| ATI2 UK Limited | Professional & scientific | 2018 |
| Atkinson Communications Ltd | Information & communication | 2019 |
| Atkinson HR Consulting Limited | Professional & scientific | 2017 |
| Attribute Digital Ltd | Professional & scientific | 2005 |
| AUM Store Limited | Wholesale & retail | 2024 |
| Aurillie Belle Ltd | Wholesale & retail | 2025 |
| Aurumd by KC Limited | Hospitality | 2025 |
| Automatedtechnologies Ltd | Manufacturing | 2010 |
| Autorim Limited | Extraterritorial | 2026 |
| Avant Accounts Limited | Professional & scientific | 2023 |
| Avanti Abruzzo Ltd | Administrative & support | 2019 |
| Avanti Conveyors Limited | Manufacturing | 1998 |
| Avenue 85 Limited | Wholesale & retail | 2008 |
| Avid Roofing Limited | Construction | 2014 |
| Avila Automotive Limited | Administrative & support | 2009 |
| Awaken Events Limited | Health & social care | 2025 |
| Awaken With Elesha Limited | Wholesale & retail | 2021 |
| AWS Bathroom Supplies Limited | Wholesale & retail | 2014 |
| AXE Head Property Limited | Real estate | 2016 |
| Axon Garside & Company Limited | Professional & scientific | 1975 |
| Azaan MCR Limited | Hospitality | 2025 |
| B P C Building Products Limited | Manufacturing | 1977 |
| B. Fowler Tree and Groundwork Ltd | Administrative & support | 2025 |
| B. Maybin Carpentry and Joinery Specialists Limited | Construction | 2007 |
| Bacezza Ltd | Administrative & support | 2011 |
| Badgerwood Holdings Ltd | Financial services | 2026 |
| Bakehurst Gardens Management Ltd | Households | 2021 |
| Bakewell MOT Centre Limited | Manufacturing | 2004 |
| Bakewell Pottery Painting Ltd | Arts & recreation | 2025 |
| BAL Associates Limited | Financial services | 2007 |
| Balanced Psychology Ltd | Health & social care | 2020 |
| Bank END Properties 1 Limited | Professional & scientific | 2005 |
| Bank END Properties 2 Limited | Professional & scientific | 2005 |
| Bare Recruitment Limited | Administrative & support | 2016 |
| Barratt's Bakery Ltd | Manufacturing | 2025 |
| Basbug Ltd | Hospitality | 2023 |
| Bathrooms by Fountain T/A Fountain Kitchens and Bathrooms Ltd | Wholesale & retail | 2023 |
| Beardwood Natural Living Project C.I.C. | Health & social care | 2011 |
| Beauty by JAY Ltd | Other services | 2019 |
| Become Consulting Limited | Professional & scientific | 2016 |
| Beetee Developments Ltd | Real estate | 2016 |
| Being Outdoors CIC | Education | 2020 |
| Bele Bakehouse Limited | Hospitality | 2026 |
| Bella at the Bridge Limited | Real estate | 2026 |
| Bellowflex UK Limited | Professional & scientific | 2023 |
| Beyond Borders - Structures and Landscapes Limited | Administrative & support | 2025 |
| Bhcra Limited | Administrative & support | 1991 |
| Biaggi UK Ltd | Wholesale & retail | 2023 |
| Bianco Rosso Ltd | Hospitality | 2010 |
| BIO Mandala 108 Ltd | Wholesale & retail | 2025 |
| Biopharma Growth Partnership Ltd | Professional & scientific | 2024 |
| Biosimetrics Limited | Information & communication | 2014 |
| Birch Vale Community Interest Company | Professional & scientific | 2021 |
| Bird Guard UK Ltd | Administrative & support | 2025 |
| Black Circle Real Estate Limited | Real estate | 2025 |
| Black Shield Ltd | Administrative & support | 2025 |
| Blackcomb Limited | Information & communication | 2008 |
| Blake's Property Holdings Limited | Households | 2022 |
| Blakethorn Limited | Real estate | 2005 |
| Bloom FM Limited | Professional & scientific | 2017 |
| Blossomz Catering Services Limited | Hospitality | 2025 |
| Blue Grass Purple COW Limited | Health & social care | 2004 |
| Blue Moon Spiritual Well-Being Ltd | Extraterritorial | 2019 |
| Blue Straggler Ltd | Professional & scientific | 2017 |
| Blue Whale Consult Ltd | Professional & scientific | 2025 |
| Bluebird's Nest Ltd | Wholesale & retail | 2025 |
| Blueprint Learning Ltd | Education | 2025 |
| Blus Bars Ltd | Hospitality | 2025 |
| BMA Consulting Ltd | Financial services | 2020 |
| BOD Buy Limited | Wholesale & retail | 2025 |
| Bohemian Catering Company Ltd | Hospitality | 2020 |
| Bokor Highpeak Ltd | Hospitality | 2026 |
| Bollington Consultancy Services Ltd | Real estate | 1999 |
| Bonetti Homeware Limited | Wholesale & retail | 2023 |
| Boombeam Limited | Professional & scientific | 2015 |
| Bouchers Accountants Limited | Professional & scientific | 2024 |
| Bowden Theatre Works Community CIC | Arts & recreation | 2026 |
| Bpmnm Ltd | Hospitality | 2017 |
| Bradbury Logistics Limited | Transport & logistics | 2010 |
| Brady Graham Consultancy Limited | Professional & scientific | 2018 |
| Bramley's Construction & Landscaping Ltd | Construction | 2024 |
| Brand Secure Ltd | Information & communication | 2026 |
| Braseco Export UK Ltd | Wholesale & retail | 2015 |
| Brendan Pattison and SON Limited | Construction | 2015 |
| Bridge & Barrel Ltd | Hospitality | 2026 |
| Bridge 42 Ltd | Hospitality | 2025 |
| Bridge Packaging Ltd | Administrative & support | 2019 |
| Brindsett Limited | Wholesale & retail | 1979 |
| British Spirals and Castings Limited | Extraterritorial | 2014 |
| Brooklands Sheet Metal Limited | Manufacturing | 2015 |
| Brooklyn Engineering Ltd | Professional & scientific | 2024 |
| Brooks Joinery Manufacturers Ltd | Construction | 2019 |
| Brooks Multimedia Limited | Information & communication | 2015 |