| ROG SHE Muzique Limited | Manufacturing | 2024 |
| Roller Energy UK Ltd | Arts & recreation | 2025 |
| Rome Estates Ltd | Real estate | 2024 |
| Romiley Sweets & Shakes Limited | Wholesale & retail | 2025 |
| Roscoe ANP Limited | Health & social care | 2016 |
| Rosehead Limited | Real estate | 1989 |
| Rotaflow Group Limited | Manufacturing | 2013 |
| Rotaflow OIL & GAS Limited | Manufacturing | 2013 |
| Rough Spots Limited | Administrative & support | 2017 |
| Round the Corner Properties Limited | Real estate | 2022 |
| RRM Advisory Ltd | Professional & scientific | 2022 |
| Rubicon Watch Company Limited | Other services | 2013 |
| Rumination Limited | Information & communication | 2026 |
| RW Fire Protection Ltd | Construction | 2021 |
| Ryans DIY Centre Limited | Wholesale & retail | 2004 |
| Ryeflatt Interiors Limited | Extraterritorial | 2000 |
| Ryland Property Development Ltd | Real estate | 2016 |
| S & W Engineering (NW) Limited | Professional & scientific | 2023 |
| S Allsop Engineering Ltd | Construction | 2018 |
| S L Broadbent Decorators Ltd. | Construction | 2003 |
| S Robinson Consulting Limited | Administrative & support | 2025 |
| S&M Herling Limited | Hospitality | 2021 |
| S&S Thruster Services Ltd | Professional & scientific | 2019 |
| S. Drinkwater & SON Limited | Wholesale & retail | 1996 |
| Sack and Sage Ltd | Manufacturing | 2025 |
| Saha Retail Ltd | Wholesale & retail | 2019 |
| Saint Lazar Limited | Wholesale & retail | 2025 |
| Sally Eccleston Psychology and Wellbeing Limited | Education | 2021 |
| Salthouse Stonemasonry Ltd | Manufacturing | 2024 |
| Samantha Wood Theatre Academy Ltd | Arts & recreation | 2025 |
| Sandbrook Enterprise Ltd | Hospitality | 2025 |
| Sarah Turner Therapies Ltd | Health & social care | 2022 |
| Satellite Oceanographic Consultants Limited | Professional & scientific | 2008 |
| Saunders Property Solutions Ltd | Real estate | 2019 |
| Sbjm Limited | Construction | 2020 |
| Scandrea Limited | Administrative & support | 2016 |
| SCI Forte Limited | Professional & scientific | 2012 |
| Scichain Solutions Ltd | Professional & scientific | 2023 |
| Scorebox Limited | Wholesale & retail | 2025 |
| Scott Boden Limited | Arts & recreation | 2021 |
| Scrol Technology Ltd | Information & communication | 2026 |
| Sea Land and Property Ltd | Real estate | 2022 |
| Searle Building Company Limited | Real estate | 2012 |
| Securaip Ltd | Construction | 2020 |
| Sedgley Healthcare Limited | Other services | 1997 |
| Seneca Drives Limited | Professional & scientific | 2008 |
| Senior (Building Supplies) Limited | Wholesale & retail | 1996 |
| Sergiu Jiduc Consulting Ltd | Professional & scientific | 2020 |
| SET in Stone (UK) Limited | Manufacturing | 2012 |
| Sett Developments Ltd | Real estate | 2021 |
| Sett Valley Cafe Limited | Hospitality | 2019 |
| SEVENTY7 Trading Limited | Arts & recreation | 2024 |
| Shack Estates Ltd | Real estate | 2024 |
| Shared Realities Limited | Information & communication | 2020 |
| Sharp Performance and Nutrition Limited | Health & social care | 2021 |
| Shawview Consulting Ltd | Professional & scientific | 2017 |
| Sheldon Decoration Limited | Other services | 2003 |
| Sheldon Joinery Limited | Construction | 2007 |
| Shem Services Ltd | Professional & scientific | 2022 |
| Shepley's Family Butcher Limited | Wholesale & retail | 2020 |
| SHOP4FORTE Ltd | Wholesale & retail | 2024 |
| Shroy Limited | Construction | 2015 |
| Sidco (1953) Ltd | Real estate | 2006 |
| Sidco Energy Services Ltd | Wholesale & retail | 2006 |
| Sidco Power Services Limited | Real estate | 2022 |
| Signs Made Here Limited | Manufacturing | 2010 |
| Silverdale Cottages Ltd | Hospitality | 2025 |
| Silversound Productions Limited | Information & communication | 2007 |
| Simbal Holdings Limited | Professional & scientific | 2018 |
| Simbal Limited | Wholesale & retail | 1979 |
| Simbal North West Limited | Professional & scientific | 2010 |
| Simple Art Marketing Ltd | Information & communication | 1991 |
| Simply Better IT Limited | Information & communication | 2020 |
| Simply Shetlands Ltd | Health & social care | 2025 |
| SJ Design Ltd | Construction | 2007 |
| SJK Nursing & Healthcare Consultant Limited | Professional & scientific | 2007 |
| Sjlt Services Limited | Professional & scientific | 2004 |
| SJW Property Investments Limited | Real estate | 2016 |
| SK Exterior Power Cleaning Ltd | Administrative & support | 2024 |
| SK Songwriters CIC | Information & communication | 2025 |
| SK Van Repairs Ltd | Wholesale & retail | 2026 |
| SK7 Construction Limited | Construction | 2009 |
| Skills Local Ltd | Professional & scientific | 2015 |
| Skin by SRT Ltd | Other services | 2026 |
| Skin Solution Clinic Limited | Other services | 2011 |
| SKJ Advisory Limited | Professional & scientific | 2025 |
| Slater Jennings Properties Limited | Real estate | 2022 |
| Slaterwilde Limited | Professional & scientific | 2011 |
| Slushypro Limited | Wholesale & retail | 2026 |
| Smart Wire Solutions Ltd | Construction | 2022 |
| SMD Enterprise Ltd | Wholesale & retail | 2020 |
| Smith Contractors Ltd | Administrative & support | 2013 |
| Smithyfield Limited | Professional & scientific | 2023 |
| Solar Point Services Limited | Construction | 2006 |
| Soothe Day Spa Ltd | Other services | 2025 |
| Sorpra Limited | Professional & scientific | 2025 |
| South Manchester Commercial Vehicle Sales Ltd | Wholesale & retail | 2019 |
| Southwell City Football Club Limited | Arts & recreation | 2024 |
| SPB Construction Ltd | Construction | 2022 |
| Speedycom Performance Limited | Wholesale & retail | 2004 |