| Shelbourne & Greer Ltd | Construction | 2022 |
| Shorts of Hawick Holdings Limited | Financial services | 2023 |
| Shorts of Hawick Limited | Manufacturing | 1998 |
| Simon Richard Limited | Wholesale & retail | 1997 |
| SIR Engineering Ltd | Professional & scientific | 2017 |
| Sitootary Solar Limited | Energy | 2025 |
| SJM Coaching Ltd | Arts & recreation | 2016 |
| Sllp 392 Limited | Administrative & support | 2023 |
| Softaware (Scotland) Limited | Information & communication | 2004 |
| Solarcan Ltd | Manufacturing | 2021 |
| Southern Cross Construction Limited | Construction | 2023 |
| Sovereign Security Solutions Services Ltd | Administrative & support | 2025 |
| Spacess Ltd | Real estate | 2021 |
| Spanish Hands Limited | Wholesale & retail | 2018 |
| Spark Stays Ltd | Information & communication | 2025 |
| Sparkle Speak Jewellery Ltd | Wholesale & retail | 2025 |
| Spirebush Ltd | Energy | 2021 |
| Spital Tower Tourism Ltd | Hospitality | 2011 |
| Spittal on Rule Racing Limited | Arts & recreation | 2020 |
| SRS Care Solutions (Scotland) Ltd | Health & social care | 2017 |
| ST Ronan’S Well & Water Co Ltd | Other services | 2025 |
| ST. Abbs Select Limited | Hospitality | 2021 |
| Stablecroft Conference Products Limited | Manufacturing | 2010 |
| Standhill Renewables Limited | Energy | 2010 |
| Star Barbers Hawick Ltd | Other services | 2024 |
| Stela Limited | Health & social care | 2021 |
| Stell Renewables Ltd | Administrative & support | 2024 |
| Stephen Darling Limited | Construction | 2021 |
| Stewart Christie & Co (Holdings) Limited | Real estate | 1961 |
| Stickshed Limited | Construction | 2025 |
| Strawberry Lemonade Events and Hire Ltd | Administrative & support | 2024 |
| Strawberry Lemonade Events Ltd | Arts & recreation | 2018 |
| Streat Drycom Ltd | Manufacturing | 2020 |
| Strength Labs Gyms Ltd | Arts & recreation | 2026 |
| Sultaans Restaurant (Hawick) Ltd | Hospitality | 2020 |
| Sweet Red Balls Limited | Agriculture & fishing | 2010 |
| T B Oliver Limited | Manufacturing | 2012 |
| Talo Estates Ltd | Real estate | 2019 |
| Talo Property Management Ltd | Real estate | 2017 |
| TCB Hawick Ltd | Wholesale & retail | 2020 |
| TD9 Consulting Limited | Administrative & support | 2025 |
| Teviot Ewes Designs Ltd | Manufacturing | 2023 |
| Teviot Eyecare Ltd | Wholesale & retail | 2022 |
| Teviot Knitwear Hawick Ltd | Manufacturing | 2020 |
| Teviot Taxi Limited | Transport & logistics | 2024 |
| Teviot Tyres & MOT Centre Limited | Wholesale & retail | 2024 |
| Teviotdale Holdings Limited | Financial services | 2022 |
| Teviotside Travel Inn Ltd | Hospitality | 2023 |
| THE Backload Network Limited | Transport & logistics | 2024 |
| THE BE Inspired Group Ltd | Professional & scientific | 2013 |
| THE Bill Mclaren Foundation | Arts & recreation | 2010 |
| THE Borders Distillery Company Limited | Manufacturing | 2013 |
| THE Borders Green Team Enterprises | Administrative & support | 2006 |
| THE Borders Whisky Company Limited | Manufacturing | 2015 |
| THE Chisholme Institute | Education | 1979 |
| THE Firm of Hoscote Farming Partnership | | 1994 |
| THE Hawick Cashmere Company Limited | Manufacturing | 1990 |
| THE Hawick Paper Ltd | Manufacturing | 2016 |
| THE Hawick Scarf Company Limited | Manufacturing | 2006 |
| THE Hawick Tartan & Tweed Company Ltd | Wholesale & retail | 2026 |
| THE Lowrie Property Group Ltd | Financial services | 2022 |
| THE Marketeers (Scotland) Ltd | Professional & scientific | 2023 |
| THE Office (Hawick) Limited | Hospitality | 2015 |
| THE Parlour Room Collective CIC | Education | 2025 |
| THE Stair Building Company Ltd | Construction | 2021 |
| THE Town O' Rule Partnership | | 1999 |
| THE Trinity Handmade Company Ltd | Wholesale & retail | 2017 |
| THE Voice of Rugby Limited | Arts & recreation | 2010 |
| THE Waverley Bar Limited | Hospitality | 2003 |
| THE Write Reasons Ltd | Administrative & support | 2026 |
| Thewatchroom.Com Ltd | Wholesale & retail | 2022 |
| This IS Counter Culture Ltd | Wholesale & retail | 2025 |
| Thomas Brisbane & Sons Limited | Construction | 2010 |
| Thought Research Limited | Professional & scientific | 2014 |
| Tiny Racing Car Models Ltd | Wholesale & retail | 2024 |
| Tony's Takeaway Hawick Ltd | Hospitality | 2026 |
| Toprak Cafe Ltd | Hospitality | 2025 |
| Towerdykeside Limited | Professional & scientific | 2024 |
| Town of 1000 Trails C.I.C. | Information & communication | 2020 |
| Trowmill Enterprises Ltd | Manufacturing | 2021 |
| Tullyfergus Agriculture Ltd | Agriculture & fishing | 2021 |
| Turnbull & Scott (Engineers) Limited | Manufacturing | 2007 |
| Turnbull & Scott Trustees Limited | Professional & scientific | 2015 |
| Two Hearts Care Company Ltd | Health & social care | 2026 |
| UK Classified Hub Ltd | Professional & scientific | 2025 |
| Unintelligent Systems Ltd | Hospitality | 2025 |
| Varnsen Consulting Limited | Professional & scientific | 2014 |
| Veevli Limited | Administrative & support | 2016 |
| Veritas Curat Limited | Health & social care | 2018 |
| W Amos & Company | | 1988 |
| W E Howden Ltd | Wholesale & retail | 2021 |
| Wahnfried AI Ltd | Information & communication | 2026 |
| Wanderfree Ltd | Wholesale & retail | 2024 |
| Watermark Events Ltd | Hospitality | 2022 |
| Waugh and Mckean Limited | Construction | 2009 |
| Waukrigg Development Company Limited the | Real estate | 1984 |
| Waverley Housing | Real estate | 1988 |
| Waxy's Emporium Ltd | Wholesale & retail | 2024 |
| Weensland Motor Centre Limited | Wholesale & retail | 2012 |
| Wei's Ltd | Hospitality | 2008 |