| Optomtom Limited | Wholesale & retail | 2011 |
| Orivian Ltd | Health & social care | 2025 |
| Ormiston Farm Partnership | | 1990 |
| Outdoors With Nature CIC | Health & social care | 2021 |
| P.H. Reddihough & Partner | | 1992 |
| P.R Gabriel Ltd | Health & social care | 2021 |
| Panda Equity Ltd | Professional & scientific | 2010 |
| Panda Motorsport Ltd | Extraterritorial | 2011 |
| Panda Racing Limited | Extraterritorial | 2009 |
| Pandamonium Racing Limited | Wholesale & retail | 2010 |
| Parme Limited | Wholesale & retail | 2021 |
| Passmore Investments Limited | Administrative & support | 2021 |
| Paterson Gardens Management Company Ltd | Households | 2025 |
| Paul Henry Brickwork Ltd | Construction | 2024 |
| Paul Kenny Ltd | Professional & scientific | 2002 |
| Peter Graham Contracts Ltd | Construction | 2023 |
| Phatt Bois Catering Ltd | Hospitality | 2024 |
| Philip Consulting Ltd | Professional & scientific | 2017 |
| Platinum Holiday Lets Limited | Hospitality | 2019 |
| Point Procurement Ltd | Wholesale & retail | 2024 |
| Polylabel UK Ltd. | Manufacturing | 2011 |
| Potential Trade Ltd | Wholesale & retail | 2023 |
| Potentiality Consulting Limited | Professional & scientific | 2003 |
| Powertec Electrotechnical Services Limited | Construction | 2010 |
| Precise Mastering Ltd | Information & communication | 2011 |
| Prettyfresh Health & Fitness Ltd | Wholesale & retail | 2023 |
| Primo Landscaping Limited | Administrative & support | 2007 |
| Pringle of Scotland Limited | Wholesale & retail | 2000 |
| Priory Removals & Transport Ltd | Transport & logistics | 2025 |
| Protetch Ltd | Manufacturing | 2008 |
| PSL Scotland Limited | Arts & recreation | 2025 |
| Punjabi Grill Hawick Ltd | Hospitality | 2021 |
| Purple Sage Soap Company Ltd | Manufacturing | 2021 |
| PW Piling Ltd | Administrative & support | 2020 |
| PZ Smiles Ltd | Health & social care | 2025 |
| QH Hawick Limited | Hospitality | 2019 |
| Queen's Mire Limited | Extraterritorial | 2012 |
| R & B Painters & Decorators Ltd | Construction | 2016 |
| R & H Swailes Limited | Agriculture & fishing | 2022 |
| R a Gibson Electrical Limited | Construction | 2021 |
| R a Oliver Motor Mechanic Ltd | Wholesale & retail | 2020 |
| R Gillespie Groundworks Limited | Construction | 2008 |
| R H Brunton & Co (Hassendean) | | 1988 |
| R H Groundworks Limited | Agriculture & fishing | 2009 |
| R M Autoclaves Limited | Professional & scientific | 2003 |
| R R Fraser & SON Ltd | Construction | 2009 |
| R. & L. Howat & Co. | | 1988 |
| Radiance by Ruth Limited | Other services | 2023 |
| Raedyn Redpath Recycling Limited | Water & waste | 2021 |
| Rae’S Remapping & Automotive Key Solutions Ltd | Wholesale & retail | 2025 |
| Ragman Collective Ltd | Administrative & support | 2024 |
| Rasa Cleaners Ltd | Administrative & support | 2025 |
| Rattrig Ltd | Financial services | 2023 |
| Raze Construction Services Ltd | Construction | 2024 |
| RC Media Ltd | Arts & recreation | 2025 |
| RC Security Solutions Ltd | Administrative & support | 2024 |
| RDCONSULTS21 Ltd | Administrative & support | 2021 |
| Recovery Coaching Scotland CIC | Education | 2021 |
| Relax Plumbing&Heating Ltd | Construction | 2023 |
| Ressurect Ltd | Real estate | 2026 |
| Richard Lynn Ltd. | Construction | 2003 |
| Riskren Ltd | Professional & scientific | 2024 |
| River and Green Limited | Administrative & support | 2008 |
| Rivo Plumbing and Heating Ltd | Construction | 2024 |
| RLG Properties Ltd | Real estate | 2019 |
| RLL Renewables Ltd | Administrative & support | 2023 |
| Roadhouse Vets Ltd | Professional & scientific | 2025 |
| Robert Scott Joinery Ltd | Construction | 2018 |
| Robertson Interior Renovations Ltd | Construction | 2026 |
| Rock and Metal Alliance CIC | Education | 2025 |
| Rodger & Co. | | 1978 |
| Rogue Biscuits Ltd | Information & communication | 2026 |
| Ronsand Ltd | Hospitality | 2019 |
| Rose Blythe Mortgage Solutions Ltd | Financial services | 2021 |
| Rose Cafe Hawick Ltd | Hospitality | 2025 |
| Rose Instrumentation and Control Ltd | Professional & scientific | 2013 |
| Ross Macleod GAS Services Ltd | Construction | 2023 |
| Roxanne Renwick Ltd | Wholesale & retail | 2025 |
| Roxburgh and Berwickshire Citizens Advice Bureau | Information & communication | 2011 |
| Roxburgh Heating Limited | Construction | 2012 |
| Roxburghe Handknits Limited | Manufacturing | 2004 |
| Roxburghe Home Solutions Limited | Manufacturing | 2011 |
| Rsrenergy Ltd | Other services | 2025 |
| RTE Developments Ltd | Real estate | 2018 |
| Ryan Gillan Groundworks Ltd | Construction | 2026 |
| Ryan Patterson Electrician Limited | Real estate | 2017 |
| Ryerigg Developments Limited | Other services | 1999 |
| S R Gardiner Ltd | Construction | 2021 |
| Saffron Tandoori Ltd | Hospitality | 2013 |
| Salt N Fire (Kelso) Limited | Hospitality | 2024 |
| Salt N Fire Limited | Hospitality | 2022 |
| SAN Marco Holdings Ltd | Manufacturing | 2024 |
| Sarah Laing's Country Catering Ltd | Hospitality | 2022 |
| Saturday Polish School Hawick C.I.C. | Education | 2016 |
| Schneider Property Maintenance Ltd | Households | 2025 |
| Sciencemedical Ltd | Professional & scientific | 2024 |
| Scottish Borders Chamber of Commerce C.I.C. | Other services | 2004 |
| Scottsdale Cashmere Limited | Manufacturing | 2016 |
| Sergio's of Hawick Ltd | Hospitality | 2019 |
| Shawz Home Solutions Ltd | Construction | 2024 |