| 2AB Civils and Rail Services Ltd | Construction | 2025 |
| 3R Energy Solutions Limited | Manufacturing | 2009 |
| 3R Renewables Ltd | Professional & scientific | 2006 |
| 3RE Power Limited | Energy | 2012 |
| @ the Riversedge(Hawick) Limited | Wholesale & retail | 2018 |
| A G Autotech Ltd | Wholesale & retail | 2017 |
| A Howden Ltd | Construction | 2013 |
| A J Learmonth Butcher Ltd. | Wholesale & retail | 2010 |
| A K H 5663 Limited | Hospitality | 2024 |
| A L Swailes Forestry Ltd | Agriculture & fishing | 2025 |
| A Oliver & Sons Joiners Ltd | Construction | 2017 |
| A Positive Start CIC | Other services | 2017 |
| A Thomson Plumbing & Heating Services Ltd | Construction | 2014 |
| A&J Robson Limited | Other services | 2011 |
| A.S. Crawford Groundworks Limited | Construction | 2002 |
| A1 Border Moves Limited | Transport & logistics | 2021 |
| A1 Taxi Hawick Limited | Transport & logistics | 2023 |
| Aafastfoodsuk Ltd | Hospitality | 2025 |
| AB Obice Saevior Ltd | Agriculture & fishing | 2020 |
| Abbeyside Consultancy Limited | Professional & scientific | 2007 |
| Aberdeen Student Rentals Limited | Real estate | 2023 |
| ACM Holdings Ltd | Real estate | 2023 |
| Advent Plumbing & Heating Ltd | Construction | 2021 |
| AK Orchard Limited | Other services | 2024 |
| Alan Shiel Limited | Real estate | 2002 |
| Alderbank Joinery Ltd | Construction | 2016 |
| Amarone Italian Restaurant Ltd | Hospitality | 2025 |
| Amelgo Properties Ltd | Real estate | 2025 |
| Amgg Ltd | Professional & scientific | 2018 |
| Amgl Scaffolding and Access Ltd | Construction | 2026 |
| Andrew Armstrong & Sons Limited | Wholesale & retail | 1958 |
| Anua Clean Air UK Limited | Energy | 2017 |
| Ardl Holdings Ltd | Real estate | 2018 |
| Aret Advisory Ltd | Administrative & support | 2021 |
| Aris Properties Limited | Real estate | 1965 |
| Armstrongs Garage Limited | Wholesale & retail | 2013 |
| Arowyn & Co UK Ltd | Wholesale & retail | 2024 |
| ASM Developments Limited | Real estate | 1984 |
| Assetlife Alliance UK Ltd | Professional & scientific | 2021 |
| Auld Cross Keys Inn Limited | Hospitality | 2010 |
| AYE Deal Ltd | Other services | 2014 |
| Bangar Ltd | Transport & logistics | 2025 |
| Bannerman Burke Law Limited | Professional & scientific | 2023 |
| Bannerman Burke Properties Limited | Real estate | 2011 |
| Barbara Cvitanovic Ltd | Health & social care | 2025 |
| Barnhills Farm | | 1987 |
| Bathgate and Daughter Ltd | Hospitality | 2026 |
| Bathroom Planet Holdings Ltd | Financial services | 2021 |
| Bayhill Holding Company Ltd | Agriculture & fishing | 2018 |
| Berks Supply Ltd | Wholesale & retail | 2026 |
| Beverley's Wedding Boutique Ltd | Wholesale & retail | 2024 |
| Bizzyberry Nursery Ltd | Health & social care | 2015 |
| Blair & Patterson Holdings Limited | Professional & scientific | 2005 |
| Blair & Patterson Home Improvements Limited | Manufacturing | 2007 |
| Blair & Patterson Manufacturing Limited | Manufacturing | 2012 |
| Border and City Contracts Ltd | Construction | 2024 |
| Border Autocare & Tyre Services Limited | Wholesale & retail | 2001 |
| Border Bricklayers Ltd | Construction | 2022 |
| Border Farms | | 1987 |
| Border Insulation Ltd | Construction | 2023 |
| Border Landscaping & Tree Services Ltd | Administrative & support | 2015 |
| Border Memorials Limited | Construction | 2023 |
| Border Mouldings Ltd | Manufacturing | 2017 |
| Border Stoves Ltd | Construction | 2023 |
| Border Women's AID Ltd | Health & social care | 2011 |
| Borderland UK Ltd | Wholesale & retail | 2021 |
| Borders Aesthetics Ltd | Other services | 2021 |
| Borders Clearance and Maintenance Ltd | Transport & logistics | 2024 |
| Borders Distillers Limited | Manufacturing | 2015 |
| Borders Distilling Limited | Manufacturing | 2017 |
| Borders Environmental Enterprises Ltd | Administrative & support | 2017 |
| Borders Sparkle Cleaning Ltd | Administrative & support | 2026 |
| Borders Waste Solutions Ltd | Administrative & support | 2026 |
| Borthwick Groundworks Limited | Construction | 2017 |
| Borthwickbrae Farms | | 1981 |
| Borthwickbrae Farms | | 1993 |
| Bowholm Holdings Limited | Professional & scientific | 1990 |
| Brandon Cyclesports Ltd | Extraterritorial | 2025 |
| Brandon Investment Group Ltd | Extraterritorial | 2011 |
| Branxholm Park House Limited | Other services | 2019 |
| Branxholme Castle Limited | Hospitality | 2017 |
| Branxholme Holiday Lets Limited | Hospitality | 2023 |
| Brighté Studio Ltd | Wholesale & retail | 2026 |
| Brown and Turner Ltd | Wholesale & retail | 2017 |
| Browns ASR Limited | Wholesale & retail | 2005 |
| Bruce Lynn Ltd | Arts & recreation | 2024 |
| BSM Contracts Ltd | Construction | 2021 |
| BSM Property Developments Ltd | Construction | 2025 |
| Bucklands Garden Ltd | Agriculture & fishing | 2026 |
| Bucklands Nest Ltd | Agriculture & fishing | 2026 |
| Burnfoot Community Futures | Other services | 2008 |
| C D Thundow Agencies Ltd | Wholesale & retail | 2022 |
| C Liddle Ltd | Administrative & support | 2021 |
| C&C Property Retreats Ltd | Households | 2025 |
| Calaburn Renewables Limited | Administrative & support | 2023 |
| Caledonia LOG Homes Limited | Manufacturing | 2011 |
| Call Ltd | Transport & logistics | 2025 |
| Campbell & Lowrie Joinery Ltd | Construction | 2019 |
| Campbell Capital Ltd | Financial services | 2018 |
| Cannon Central Heating Ltd | Construction | 2025 |