| Carlingnose Associates Limited | Financial services | 2007 |
| Caroline Teltsch Public Relations & Event Management Limited | Administrative & support | 2002 |
| Caspian Kebab Hawick Ltd | Hospitality | 2018 |
| Caswell Forestry Ltd | Agriculture & fishing | 2010 |
| Catford Investments Limited | Real estate | 2012 |
| Cavers Estate Ltd | Real estate | 2012 |
| Celeanor Ltd | Professional & scientific | 2019 |
| Cheasy Ltd | Information & communication | 2012 |
| Chesters Footwear Limited | Wholesale & retail | 2018 |
| Chips Away Ltd | Hospitality | 2024 |
| Chrysties Furnishing Centre Ltd | Other services | 2014 |
| Chrysties Properties Ltd | Financial services | 2021 |
| CK Textile & General Engineering Ltd | Manufacturing | 2014 |
| Claire Maclachlan Copywriting Limited | Administrative & support | 2020 |
| Clamp & Pringle (Housebuilders & Contractors) Ltd | Construction | 2003 |
| Clamp Holdings Ltd | Financial services | 2022 |
| Clamp Property Ltd | Real estate | 2022 |
| Clarks of Hawick Ltd | Construction | 2026 |
| Cleo's Nails & Beauty Ltd | Other services | 2025 |
| Clinkscale Coaching Limited | Administrative & support | 2017 |
| Cloud Vapes & Convenience Ltd | Wholesale & retail | 2024 |
| Coffee Corner (Jedburgh) Ltd | Hospitality | 2017 |
| Coffee END Ltd | Hospitality | 2024 |
| Coliforthill Farm Partnership | | 1990 |
| Commercial Electric Heat Limited | Extraterritorial | 2018 |
| Connected Landscapes Limited | Professional & scientific | 2023 |
| Cooper Bar Ltd | Hospitality | 2012 |
| Coopers Bar 2026 Ltd | Hospitality | 2026 |
| Core 3 Partners Limited | Manufacturing | 2008 |
| Core Procurement Ltd. | Wholesale & retail | 2022 |
| Coshark Ltd | Real estate | 2024 |
| Country Cards Holdings Limited | Wholesale & retail | 2019 |
| Country Cards UK Limited | Manufacturing | 2006 |
| Cover ALL Bases Limited | Health & social care | 2019 |
| CR8 Flooring Limited | Construction | 2008 |
| Craig Motors Limited | Wholesale & retail | 2008 |
| Craighill Contracts Limited | Mining & quarrying | 2020 |
| Craikhope Community Interest Company | Hospitality | 2018 |
| D & GLS Limited | Real estate | 2026 |
| D a Oliver Limited | Construction | 2013 |
| D Swailes Forestry Ltd | Agriculture & fishing | 2026 |
| D. Charters Electrical Services Limited | Construction | 2003 |
| D.H. Anderson & Co (East Buccleuch) | | 1991 |
| DAC 1964 Holdings Ltd | Financial services | 2020 |
| Dalia Mansour Limited | Hospitality | 2023 |
| Dalton Redpath Surfacing Limited | Construction | 2019 |
| David Corrie Joinery Ltd | Construction | 2025 |
| David Reid Roofing Limited | Construction | 2015 |
| Dces Holdings Ltd | Financial services | 2016 |
| Deans Accountants and Business Advisors Ltd. | Professional & scientific | 1998 |
| Deans Enterprises (Hawick) Limited | Real estate | 1974 |
| Deans Investment Advisors LLP | | 2006 |
| Deans Secretaries Ltd. | Financial services | 2005 |
| Delooze Hearing Ltd | Wholesale & retail | 2023 |
| Denholm Food Store Ltd | Wholesale & retail | 2023 |
| Detecting in Depth Ltd | Administrative & support | 2026 |
| Discovery Property Group Ltd | Real estate | 2025 |
| DL Electrical & Renewables Limited | Construction | 2022 |
| Dogs & Duvets Respite Care Ltd | Other services | 2026 |
| Donald Stewart Ltd | Financial services | 2022 |
| Douglas Autocare Limited | Administrative & support | 2017 |
| Douglas Page Ltd. | Construction | 2011 |
| Douglas-Hamilton Properties | Administrative & support | 2008 |
| Dovestane LLP | | 2010 |
| Drummossie Estates Limited | Real estate | 1998 |
| Dundee Textiles Limited | Professional & scientific | 1997 |
| Dunsdale Demolition Ltd | Construction | 2023 |
| Dyson & Co. (Scotland) Ltd. | Wholesale & retail | 2001 |
| DZ Property Investments Ltd | Real estate | 2023 |
| E & a Retail Ltd | Wholesale & retail | 2019 |
| Earlsgate Property Investments Limited | Real estate | 2004 |
| East Coast Commissioning Limited | Mining & quarrying | 2002 |
| East Lothian Mortgage Hub Ltd | Financial services | 2018 |
| Ecoshield Roxburghe Ltd | Administrative & support | 2025 |
| Edinburgh Mortgage Hub Ltd | Financial services | 2018 |
| Eileen Randall Limited | Wholesale & retail | 2004 |
| Elite Decor (Hawick) Limited | Construction | 2021 |
| Elite Finish Plastering Ltd | Construction | 2025 |
| Elite Plumbing & Heating Services Limited | Construction | 2015 |
| Elizabeth Grace Homes UK Ltd | Construction | 2022 |
| Elliot William Mcdonald Ltd | Other services | 2016 |
| Embroidery Elite Limited | Manufacturing | 2013 |
| EMK Venture UK Ltd | Hospitality | 2025 |
| Emtelle Holdings Limited | Administrative & support | 2014 |
| Enziedi Ltd | Agriculture & fishing | 2025 |
| Epharmadirect Ltd | Other services | 2025 |
| Escape Youth Services | Education | 2012 |
| Ewan Whillans Racing Limited | Arts & recreation | 2020 |
| Excellart LLP | | 2018 |
| Exchange Dalton's Bar Ltd | Hospitality | 2026 |
| F & R Joinery Ltd | Construction | 2013 |
| FAB & Frankie Ltd | Hospitality | 2025 |
| Facd Contracting Ltd | Construction | 2017 |
| Faces by AB Ltd | Other services | 2025 |
| Fairways Solutions Limited | Construction | 2022 |
| Falconry Scotland Ltd | Arts & recreation | 2019 |
| Falnash Lodges Ltd | Hospitality | 2019 |
| Falside Farmers | | 1988 |
| Famous Paint & Decoration Joinery Ltd | Construction | 2023 |
| Famously Hawick | Administrative & support | 2020 |