| Devinez Ltd | Wholesale & retail | 2025 |
| Dhanshree Limited | Wholesale & retail | 2023 |
| DI Pola's (Sussex) Limited | Hospitality | 2010 |
| Diane Sykes Fashion Marketing Limited | Wholesale & retail | 2015 |
| Digger Rent Limited | Administrative & support | 2014 |
| Digimall Ltd | Wholesale & retail | 2024 |
| Digital World Direct Limited | Wholesale & retail | 2001 |
| Digitales Limited | Education | 2008 |
| Digiterl Networks Ltd | Information & communication | 2022 |
| Dilder Ltd | Hospitality | 2025 |
| Dillon BTL Ltd | Real estate | 2025 |
| Dink Animations Ltd | Professional & scientific | 2019 |
| Direct Draincare Services Limited | Water & waste | 2008 |
| Direct Response Copy Limited | Administrative & support | 2006 |
| Dirty Rocker Ltd | Wholesale & retail | 2026 |
| Discover Aviation Ltd. | Education | 2024 |
| Dissolved Lenny Ltd | Wholesale & retail | 2025 |
| Ditron Limited | Hospitality | 2014 |
| Divyesh Limited | Financial services | 2025 |
| DJH Scaffolding Ltd | Construction | 2022 |
| DML Governance & Compliance Ltd | Professional & scientific | 2019 |
| DNA Security Limited | Other services | 2004 |
| DNM Electrics Ltd | Construction | 2017 |
| DO Your Ideas CIC | Arts & recreation | 2024 |
| Dohickey Productions Ltd | Information & communication | 2014 |
| Dolly and Freya Ltd | Wholesale & retail | 2025 |
| Dolphin (Hastings) Limited | Hospitality | 2005 |
| Dolphin Deliciousness Limited | Hospitality | 2023 |
| Dominic Burt Carpentry Ltd | Construction | 2022 |
| Doswell Lafave Places Ltd | Real estate | 2019 |
| Dottie Ltd | Arts & recreation | 2014 |
| Douglas Rowe Financial Services Limited | Professional & scientific | 2017 |
| Downey Property Developments Limited | Administrative & support | 2004 |
| Downton Builders Limited | Construction | 2018 |
| Dradamus CIC | Arts & recreation | 2024 |
| Dragon Bars Limited | Hospitality | 2005 |
| Dragonfly Clinic Ltd | Health & social care | 2008 |
| Draupadi Jewels Ltd | Wholesale & retail | 2026 |
| Dream Machines Events Limited | Administrative & support | 2025 |
| Drive 2 Survive Network Community Interest Company | Education | 2022 |
| Drive Content Ltd | Professional & scientific | 2025 |
| Driving Performance Limited | Professional & scientific | 2023 |
| Ds-Sg Properties Limited | Real estate | 2024 |
| DSF Groundworks Ltd | Construction | 2022 |
| DSR RBI Services Ltd | Professional & scientific | 2026 |
| DT Studios Hastings Ltd | Real estate | 2023 |
| Dubreq Holdings Ltd | Financial services | 2020 |
| Dubreq Ltd | Manufacturing | 2014 |
| Duct&Acoustic Installations Ltd | Construction | 2016 |
| Dudley Pearse Psychology Services Ltd | Information & communication | 2009 |
| Duffie Architecture Limited | Professional & scientific | 2021 |
| Dumpsters Residential & Commercial Rubbish Clearance Limited | Transport & logistics | 2024 |
| Duncan & Pemble Limited | Professional & scientific | 2015 |
| Dwarka Drape Limited | Wholesale & retail | 2025 |
| Dyer & Hobbis 2 Limited | Real estate | 2018 |
| Dyer & Hobbis Investments Limited | Real estate | 2014 |
| Dyer and Hobbis Limited | Real estate | 2011 |
| Dyke & Dean Ltd | Wholesale & retail | 2011 |
| Dynamic YOU Limited | Health & social care | 2013 |
| E2WFS Ltd | Wholesale & retail | 2022 |
| E2WFS Tyres Ltd | Wholesale & retail | 2024 |
| Eaglehurst Fish Bar Limited | Hospitality | 2023 |
| Eaglehurst Limited | Real estate | 1998 |
| Eagles A&M Property Ltd | Real estate | 2022 |
| Eagles Property Management Ltd | Real estate | 2022 |
| Early Contracts Limited | Construction | 2012 |
| East Hill House Limited | Households | 2021 |
| East India Travel Company Limited | Hospitality | 2019 |
| East Sussex Buddhist Temple Ltd | Other services | 2025 |
| East Sussex Early Days CIC | Education | 2023 |
| East Sussex Haulage Limited | Transport & logistics | 2025 |
| East Sussex Lift Servicing Limited | Construction | 2008 |
| East Sussex Lifts Limited | Construction | 2006 |
| Easton Installations Limited | Construction | 2012 |
| Easy AS 123 Limited | Professional & scientific | 2017 |
| Easy Peasy Rentals Limited | Wholesale & retail | 2025 |
| EAT @... Ours Limited | Hospitality | 2004 |
| Eaters @ Pett Ltd | Hospitality | 2023 |
| Ebolov Ltd | Real estate | 2022 |
| EC Firstcare Ltd | Health & social care | 2022 |
| ECE Roofing and Cladding Ltd | Construction | 2017 |
| Echo Consulting Ltd | Hospitality | 2012 |
| ECJ Builders Limited | Construction | 2024 |
| Eclipse Procurement Limited | Professional & scientific | 2021 |
| Eclipse Procurement Solutions Limited | Professional & scientific | 2010 |
| Eco Air 1066 Limited | Construction | 2022 |
| Eco Friendly Solutions Ltd | Wholesale & retail | 2018 |
| Ecommerce Trading & Development Limited | Professional & scientific | 2020 |
| ED Plastering & DRY Lining Ltd | Construction | 2021 |
| EDQ Holdings Limited | Administrative & support | 2026 |
| Education Futures Trust | Education | 2011 |
| Education Solutions Limited | Education | 2003 |
| Edwards Carpentry Limited | Construction | 2025 |
| EEL and Bear Ltd | Wholesale & retail | 2017 |
| EFL Education and Travel (Hastings) Ltd. | Hospitality | 2015 |
| Egerton 35 Limited | Households | 2011 |
| EIM Properties (South East) Limited | Real estate | 2025 |
| Eirmed Ltd | Information & communication | 2019 |
| EJW Consultants Limited | Administrative & support | 2017 |
| Ekho Technology Limited | Information & communication | 2023 |