| Elwick Stud Limited | Agriculture & fishing | 2023 |
| EM Personal Tuition Ltd | Education | 2024 |
| EM3 Ltd | Construction | 2023 |
| Emetech Ltd | Construction | 2018 |
| Emhj Mechanical Limited | Professional & scientific | 2025 |
| Emily Nails 406 Ltd | Other services | 2023 |
| Emma’S Gardening and Maintenance Ltd | Administrative & support | 2024 |
| Emmerson Marketing Limited | Professional & scientific | 2010 |
| Empire Holdings (NE) Ltd | Financial services | 2025 |
| Empireventure Limited | Hospitality | 1987 |
| Emploshield Limited | Agriculture & fishing | 2024 |
| Emseepixels Ltd | Information & communication | 2022 |
| EN Brands Ltd | Wholesale & retail | 2026 |
| Encompass Surveyors Ltd | Professional & scientific | 2022 |
| Endeavour Building Services Ltd | Construction | 2023 |
| Energise Solutions Limited | Information & communication | 2006 |
| Energy Services (N.E) Ltd | Construction | 2007 |
| Energyservicesnortheast Ltd | Professional & scientific | 2025 |
| Englishmans Limited | Extraterritorial | 2010 |
| Enigif Group International Ltd | Wholesale & retail | 2023 |
| Enki Subsea Inspection Ltd | Construction | 2017 |
| Enterprise 2 North East Limited | Real estate | 2017 |
| Entertainments International Limited | Hospitality | 2019 |
| Enviro Group (NE) Limited | Professional & scientific | 2021 |
| EO Alliance Ltd | Construction | 2019 |
| Eolec Engineering Limited | Manufacturing | 2025 |
| EPW Print & Design Ltd | Manufacturing | 2001 |
| Erancal Limited | Professional & scientific | 2010 |
| Erimus Construction Limited | Construction | 2024 |
| Eris.Ai Limited | Information & communication | 2025 |
| Esabay Limited | Health & social care | 2023 |
| Esports Gear Limited | Wholesale & retail | 2016 |
| Essential Elements Wellbeing Ltd | Health & social care | 2025 |
| ETL Consulting Services Limited | Professional & scientific | 2025 |
| EU Radici Hydroponic Ltd | Wholesale & retail | 2022 |
| Euangelion Motors Ltd | Wholesale & retail | 2024 |
| Eurasica Ltd | Education | 2023 |
| Euro Security Limited | Administrative & support | 1999 |
| EVB Mortgages Ltd | Financial services | 2021 |
| Evectrotec Ltd | Manufacturing | 2026 |
| Evidence Informed Performance Ltd. | Information & communication | 2025 |
| Evolution Management Limited | Professional & scientific | 2017 |
| Evolution Training and Consultancy Services Limited | Administrative & support | 2014 |
| Evolve Academy in the Community CIC | Education | 2026 |
| Evolve Windows Limited | Manufacturing | 2022 |
| Ex-Assist Ltd | Administrative & support | 2024 |
| Excell Security Services (NE) Limited | Administrative & support | 2011 |
| Executive Housekeeping Ltd | Administrative & support | 2025 |
| Exon Blue Ltd | Information & communication | 2022 |
| Expamet Fencing Limited | Extraterritorial | 2002 |
| Expanded Metal Holdings Limited | Professional & scientific | 2006 |
| Extol Academy Trust | Education | 2013 |
| Extreme Line Limited | Wholesale & retail | 2019 |
| Exwold Technology Limited | Manufacturing | 1992 |
| EYE Magician Ltd | Wholesale & retail | 2025 |
| F D M Investments Limited | Manufacturing | 2018 |
| F Shotton Limited | Wholesale & retail | 1962 |
| F.C. Investments Limited | Wholesale & retail | 1982 |
| FA Memorabilia Ltd | Wholesale & retail | 2025 |
| Fabi Properties Limited | Real estate | 2025 |
| Factorycover Services Limited | Professional & scientific | 1983 |
| FAF Freight (NE) Ltd | Transport & logistics | 2023 |
| Fairfield Home Lettings Ltd | Real estate | 2024 |
| Fairlawn Teesside Ltd | Hospitality | 2016 |
| Falcon Delivery Services Ltd | Transport & logistics | 2026 |
| Falcon Electrical and PAT Testing Services Limited | Manufacturing | 2010 |
| Fallow Properties (North East) Limited | Real estate | 2021 |
| Famelia Limited | Hospitality | 2022 |
| Families First (North East) | Education | 2019 |
| Family Nails Spa Ltd | Other services | 2024 |
| Famten Limited | Real estate | 1979 |
| Farefield Property Investments Limited | Real estate | 2023 |
| Farm Fresh Market Ltd | Wholesale & retail | 2026 |
| Farrell Access Solutions Ltd | Construction | 2025 |
| Farrell IT Services Limited | Information & communication | 2016 |
| Faruk Property Service Ltd | Real estate | 2026 |
| Fasgen Limited | Manufacturing | 2023 |
| Fastfood Hartlepool Ltd | Hospitality | 2025 |
| Fayad Global Ltd | Construction | 2021 |
| Fayeben 64 Limited | Wholesale & retail | 2005 |
| Fayij Express Ltd | Hospitality | 2025 |
| FCH Enterprises Limited | Hospitality | 2021 |
| FDM Peart Limited | Manufacturing | 2018 |
| Fdsa UK Limited | Transport & logistics | 2024 |
| FEE Free Advice Ltd | Financial services | 2000 |
| Fellowship Church London | Other services | 2013 |
| Felmon Consultancy Ltd | Health & social care | 2018 |
| Fenellaverse Studios Ltd | Arts & recreation | 2026 |
| Fens Pizzeria Ltd | Hospitality | 2025 |
| Fentec - Technologies Limited | Professional & scientific | 2023 |
| Fentec Limited | Manufacturing | 2013 |
| Fernley Group NE Ltd | Energy | 2023 |
| Ferrexo Limited | Water & waste | 2019 |
| Fetch & Co Pet Services Ltd | Other services | 2024 |
| Fewster Electrical Services Ltd | Manufacturing | 2026 |
| Fewston Engineering Limited | Construction | 2026 |
| FF Build Ltd | Construction | 2021 |
| FG Services NE Limited | Energy | 2023 |
| Fibro-Connect-Ts CIC | Education | 2021 |
| Fidelity Worx Ltd | Information & communication | 2025 |