| Dolly-Ree Logistics Ltd | Transport & logistics | 2018 |
| Donkin Project Management Limited | Professional & scientific | 2022 |
| Doski Group Ltd | Other services | 2019 |
| Dot's Place Limited | Hospitality | 2017 |
| Douglas Consulting Services Limited | Financial services | 2025 |
| Douglas Hotel Ltd | Hospitality | 2025 |
| DPL Solutions Ltd. | Professional & scientific | 2023 |
| DR RG Dentistry Ltd | Health & social care | 2025 |
| Drainage Yorkshire Groundworks Ltd | Other services | 2024 |
| Drake Fibre Limited | Construction | 2024 |
| Dreamview Security Services Ltd | Construction | 2019 |
| DRF Properties Ltd | Real estate | 2021 |
| Drinkel Leisure Limited | Hospitality | 2019 |
| DRM Safety Ltd | Public administration | 2023 |
| Drop Zone Repairs Ltd | Wholesale & retail | 2025 |
| Drummond Learning Ltd | Information & communication | 2012 |
| Drunk on Ice Ltd | Hospitality | 2024 |
| DS Jointing Services Limited | Construction | 2026 |
| DSB Finance & Investments Limited | Administrative & support | 2017 |
| Dsfsafety Ltd | Professional & scientific | 2023 |
| DT Consultancy Services Limited | Administrative & support | 2017 |
| Duck House Investment Ltd | Real estate | 2020 |
| Duckhouse Solutions Ltd | Information & communication | 2022 |
| Dulai Property Limited | Real estate | 2004 |
| Duncan Atkinson Ltd | Professional & scientific | 2022 |
| Dunelm Aircon Ltd | Manufacturing | 2007 |
| Dunn Property Group North Ltd | Households | 2026 |
| Dunnett Group Consulting Ltd | Financial services | 2026 |
| Duracon Ltd | Administrative & support | 2025 |
| Durham Accident Repair Centre Limited | Extraterritorial | 2024 |
| Durham County Rugby Football Union Referees' Society Limited | Arts & recreation | 2019 |
| Durham Engineering Ltd | Professional & scientific | 2012 |
| Durham Folk Festival CIC | Arts & recreation | 2026 |
| DW FIX Ltd | Construction | 2024 |
| Dygem Limited | Construction | 2016 |
| Dynamic Training UK Limited | Professional & scientific | 2007 |
| DYS ME Limited | Professional & scientific | 2025 |
| E Andrei Solutions Ltd | Administrative & support | 2021 |
| E V S Trading Limited | Wholesale & retail | 2003 |
| E&J Jointing Limited | Construction | 2015 |
| E.V.S Contracting Services (North East) Ltd Limited | Construction | 2025 |
| E5 Engineering Limited | Administrative & support | 2008 |
| Eagle EYE Integrity Specialists Ltd. | Professional & scientific | 2016 |
| Eagle-Smt Ltd | Manufacturing | 2007 |
| Earthly Vibrations Limited | Manufacturing | 2022 |
| Easington Service Station Limited | Wholesale & retail | 2025 |
| East Durham Solutions Limited | Construction | 2018 |
| East Riding OIL Limited | Extraterritorial | 2012 |
| East View Roofing Ltd | Construction | 2024 |
| Easy-Netsuk Ltd | Manufacturing | 2012 |
| Ec&I Process Engineering Ltd | Professional & scientific | 2025 |
| Ecigracing Limited | Wholesale & retail | 2014 |
| Eclipse Surveying Limited | Professional & scientific | 2021 |
| Eco Car Sales Limited | Wholesale & retail | 2017 |
| Eco Energi Ltd | Administrative & support | 2022 |
| Eco Green Skips Limited | Water & waste | 2023 |
| Ecomm Supplies Ltd | Wholesale & retail | 2024 |
| Ecopanel Systems Limited | Administrative & support | 2003 |
| Ecru Limited | Hospitality | 2018 |
| ECS Health Limited | Health & social care | 2023 |
| ED Murray & Sons (Corus Projects) Limited | Transport & logistics | 2009 |
| Ed.Murray & Sons Limited | Manufacturing | 1971 |
| Eden Academy Trust Limited | Education | 2013 |
| Eden Education Cafe Community Interest Company | Education | 2024 |
| Eden Transport Limited | Transport & logistics | 1978 |
| EDP Rehab Limited | Health & social care | 2017 |
| Education and Send Consultancy Limited | Education | 2021 |
| Edward James Interiors (NE) Limited | Professional & scientific | 2022 |
| Eezz Care Limited | Administrative & support | 2017 |
| Eezz Global Ltd | Administrative & support | 2021 |
| Effh Holdings Limited | Real estate | 2020 |
| Egglestone Properties Limited | Real estate | 2020 |
| EGS (NE) Limited | Administrative & support | 2024 |
| EHL Express Couriers Limited | Transport & logistics | 2017 |
| EHL Group Ltd | Hospitality | 2026 |
| EJS Worth Limited | Transport & logistics | 2020 |
| EKK Completions Limited | Professional & scientific | 2023 |
| Eldon Electrical Services Limited | Mining & quarrying | 2018 |
| Eldon Grove Bowling and Tennis Club 2015 Limited | Arts & recreation | 2015 |
| Electravape North East Limited | Wholesale & retail | 2016 |
| Electrical Productions Limited | Manufacturing | 1987 |
| Electrode Electrical Services Ltd | Construction | 2018 |
| Electrosmart Limited | Wholesale & retail | 2010 |
| Electrotec Electrical Contractors Ltd | Construction | 2009 |
| Elena Solutions Limited | Professional & scientific | 2025 |
| Elevare Care Compliance Ltd | Administrative & support | 2025 |
| Elisop Engineering Services Ltd | Professional & scientific | 2026 |
| Elite Event Medical Services NE Limited | Health & social care | 2025 |
| Elite Home Care Service Limited | Health & social care | 2017 |
| Elite Outlet Direct Ltd | Wholesale & retail | 2022 |
| Elite Windings Ltd | Manufacturing | 2024 |
| Eliza Property Management Ltd | Real estate | 2023 |
| Elizabeth Beauty Care Ltd | Wholesale & retail | 2024 |
| Elkim Limited | Construction | 2002 |
| Elleve Property Ltd | Real estate | 2024 |
| Ellys Club Ltd | Information & communication | 2025 |
| Elms Commercial Properties Ltd | Real estate | 2010 |
| Eloise Lettings & Management Ltd | Real estate | 2024 |
| Elwick Homes Ltd | Construction | 2023 |
| Elwick Road Barbers Limited | Other services | 2025 |