| Route Wise Freight Ltd | Transport & logistics | 2025 |
| Rowanwood Capital Limited | Professional & scientific | 2011 |
| Roye Peters Motor Engineer Limited | Wholesale & retail | 2012 |
| Roystonhill Developments Limited | Extraterritorial | 2016 |
| RP Renewables Consultancy Limited | Construction | 2021 |
| Rpsp Invest Ltd | Real estate | 2023 |
| Rpsp Medical Ltd | Health & social care | 2023 |
| RQ Property Holdings Ltd | Real estate | 2019 |
| RR One Solutions Ltd | Construction | 2024 |
| RR Property Group Scotland Ltd | Real estate | 2023 |
| RS Elect Services Ltd | Professional & scientific | 2024 |
| RSC Trending Limited | Wholesale & retail | 2019 |
| Rsvh Limited | Wholesale & retail | 2015 |
| RT Clinical Limited | Health & social care | 2025 |
| RT Property Refurbishment Limited | Real estate | 2023 |
| RTS Transport Holding Ltd | Financial services | 2022 |
| Ruah Supply Co Limited | Manufacturing | 2026 |
| Ruby Holdings (Scotland) Ltd | Financial services | 2019 |
| Rude Raven Games Ltd | Information & communication | 2022 |
| Rufus Stone Ltd | Manufacturing | 2024 |
| Rugosa Limited | Hospitality | 2023 |
| Rumours Beauty Academy Ltd | Education | 2025 |
| Rumours Salon Limited | Other services | 2021 |
| Rural PI Limited | Professional & scientific | 2019 |
| Russell Borland Holdings Ltd | Financial services | 2023 |
| Ruthenium Property Limited | Real estate | 2017 |
| RY Cuisine Limited | Hospitality | 2026 |
| Ryan J Sullivan Properties Ltd | Real estate | 2020 |
| Ryan Mimiec Photography Ltd. | Professional & scientific | 2010 |
| S & E Hornstein Ltd. | Health & social care | 2007 |
| S & W Metal Recycling Limited | Wholesale & retail | 2021 |
| S T B a Limited | Other services | 2007 |
| S W Bakery Limited | Manufacturing | 2018 |
| S W Financial Management Ltd | Financial services | 2009 |
| S W G Golf Ltd | Arts & recreation | 2012 |
| S&B Groundworks & Garden Design Ltd | Administrative & support | 2025 |
| S&C Scaffolding/Solar Ltd | Construction | 2023 |
| S&G Electrical Wholesalers Ltd | Wholesale & retail | 2010 |
| S&S Legal Solutions Limited | Professional & scientific | 2024 |
| S'Cool for Dogs Ltd | Other services | 2024 |
| S.U.S. Water Ltd | Wholesale & retail | 2022 |
| S1 Hold Property Ltd | Financial services | 2025 |
| S1 Property Group Ltd | Real estate | 2021 |
| S6 Property Ltd | Real estate | 2015 |
| SA Palmers Green Dental Ltd | Health & social care | 2025 |
| SA Palmers Green Property Ltd | Real estate | 2025 |
| SA Private Care Ltd | Health & social care | 2023 |
| Sabarunejado-San T/A Settlers Ltd | Wholesale & retail | 2022 |
| Saccular Limited | Real estate | 2026 |
| Sachania Limited | Health & social care | 2024 |
| Sachdev Legacy Ltd | Real estate | 2021 |
| Saddlers Restaurant Ltd | Hospitality | 2024 |
| Safali Motors Ltd | Wholesale & retail | 2018 |
| Safe Heat Systems Ltd | Construction | 2021 |
| Saffronhall Properties Limited | Real estate | 2024 |
| Safsta Properties Ltd | Real estate | 2018 |
| Sagarmatha Kitchen Limited | Hospitality | 2025 |
| Sage Holdings Ltd. | | 2023 |
| Sally's Window Services Ltd | Administrative & support | 2021 |
| Salop Investments Limited | | 2023 |
| Salt Hamilton Ltd | Hospitality | 2020 |
| Salt Lanark Ltd | Hospitality | 2017 |
| Saltire Carpets & Beds Limited | Wholesale & retail | 1998 |
| Saltire Procurement Solutions Limited | Professional & scientific | 2023 |
| Sammax Contracts Limited | Construction | 2017 |
| Sammy Lemg Dog Grooming Ltd | Other services | 2020 |
| Samson Chiro Limited | Health & social care | 2019 |
| Samurguy Limited | Professional & scientific | 2024 |
| SAN Vittorio Investments Limited | Extraterritorial | 2022 |
| Sana Retail Ltd | Wholesale & retail | 2021 |
| Sana's Dream Ltd | Real estate | 2025 |
| Sanas Collection Limited | Wholesale & retail | 2022 |
| Sanda Maintenance Group Limited | Administrative & support | 2024 |
| Sandra’S Kitchen Limited | Wholesale & retail | 2020 |
| Sano Pizza Deliveries Limited | Hospitality | 2021 |
| Sano Pizza Limited | Hospitality | 2021 |
| Sapientiae Advisory Ltd | Professional & scientific | 2023 |
| Sarah Frobisher Ltd | Health & social care | 2024 |
| SAS Contracting Ltd | Construction | 2026 |
| Sasili Limited | Hospitality | 2026 |
| Sasra Business Software Solutions Ltd | Information & communication | 2014 |
| Savage Grouting Services Ltd Ltd | Construction | 2025 |
| Savan Smiles Ltd | Health & social care | 2024 |
| Savvyb Ltd | Real estate | 2021 |
| SC & EO Mcgrory Ltd | Transport & logistics | 2022 |
| SC Pubs and Investments Ltd | Financial services | 2020 |
| Scald Produce Ltd | Agriculture & fishing | 2026 |
| Scalpture Scotland Limited | Other services | 2019 |
| Scarinish Limited | Professional & scientific | 2017 |
| SCL Brickwork Ltd | Construction | 2025 |
| Scobest Ltd | Professional & scientific | 2019 |
| Scoline Ltd | Professional & scientific | 2025 |
| Scopus Holdings Limited | | 2023 |
| Scot Krav Maga Systems Ltd. | Arts & recreation | 2012 |
| Scotcal Technicians Ltd | Professional & scientific | 2015 |
| Scotia Bathrooms Ltd | Manufacturing | 2013 |
| Scotia Stateside Properties Limited | Administrative & support | 2010 |
| Scots Construction Ltd | Real estate | 2024 |
| Scots Thegither Ltd | Information & communication | 2020 |
| Scotseal Home Improvements Ltd | Construction | 2017 |