| Ekotect Ltd | Professional & scientific | 2022 |
| EL Patron Holdings Limited | Real estate | 2019 |
| ELA Site Services Ltd | Professional & scientific | 2019 |
| ELB Beauty, Skin & Aesthetics Ltd | Other services | 2021 |
| Electrace Heating Ltd | Construction | 2021 |
| Electricity Network Solutions Ltd | Construction | 2025 |
| Electro Fused Electrical Services Ltd | Manufacturing | 2023 |
| Electropoint Installations UK Ltd | Construction | 2021 |
| Elegance Chic Limited | Wholesale & retail | 2016 |
| Eleganza Sposa Limited | Real estate | 2006 |
| Elektrotechnik Ltd | Professional & scientific | 2024 |
| Element Education Ltd | Education | 2025 |
| Element Gents Ltd | Other services | 2020 |
| ELEV8 Bolton Ltd | Arts & recreation | 2024 |
| Elif 2011 Limited | Manufacturing | 2021 |
| Elite Cleaning Cleaners Ltd | Administrative & support | 2017 |
| Elite Construction (Scotland) Ltd. | Construction | 2010 |
| Elite Contracts (Scotland) Ltd. | Information & communication | 2009 |
| Elite Fire Protection Services Limited | Manufacturing | 2003 |
| Elite Property Investments (Leicester) Limited | Real estate | 2017 |
| Elite Sales Online Ltd | Wholesale & retail | 2020 |
| Elite Sports Training Ltd | Arts & recreation | 2015 |
| Elite Transmissions Limited | Manufacturing | 2021 |
| ELITE121 Ltd | Arts & recreation | 2025 |
| Elitestore Network Limited | Wholesale & retail | 2025 |
| ELK Engineering Limited | Professional & scientific | 2024 |
| Elleind Trading Limited | Other services | 2023 |
| Elliots Fruit & VEG Ltd | Wholesale & retail | 2022 |
| Elspeth Hamilton Limited | Wholesale & retail | 1988 |
| Embassy Cleaning Services Limited | Professional & scientific | 2002 |
| Emby Trading Ltd | Financial services | 2026 |
| Emerald Hire Drive Ltd | Administrative & support | 2024 |
| Emerging Asset Management Ltd | | 2008 |
| EMK Consultants Limited | Other services | 2007 |
| Emlahomes Ltd | Real estate | 2020 |
| Emotech Solutions Limited | Professional & scientific | 2012 |
| Empire Bar PUB Co Ltd | Hospitality | 2025 |
| Empire Vehicles (Scotland) Ltd | Wholesale & retail | 2026 |
| EMPOWER2TEACH Ltd | Administrative & support | 2021 |
| Empri Properties Ltd | Real estate | 2018 |
| Emtec Building Services Midlands Limited | Professional & scientific | 2016 |
| Emtec Building Services North Ltd | Professional & scientific | 2015 |
| Emtec Core & Protect Limited | Professional & scientific | 2016 |
| Emtec Energy Limited | Administrative & support | 2009 |
| Emtec Facility Services Limited | Construction | 2009 |
| Emtec Fire Systems Ltd | Construction | 2012 |
| Emtec Group Limited | Professional & scientific | 2008 |
| Emtec Group Management Ltd | Administrative & support | 2014 |
| Emtec Utility Services Limited | Construction | 2023 |
| Endless Worlds Yoga Studio Ltd | Arts & recreation | 2022 |
| Energy Certification Company Limited | Extraterritorial | 1997 |
| Energycrab Limited | Professional & scientific | 2019 |
| Enhance Capital Scotland Ltd | Other services | 2021 |
| Enhance Developments Scotland Ltd | Construction | 2012 |
| Enhance Healthcare Adam House Ltd | Health & social care | 2014 |
| Enhance Healthcare Ardencraig Ltd | Health & social care | 2025 |
| Enhance Healthcare Arran View Ltd | Other services | 2019 |
| Enhance Healthcare Burlington Limited | Health & social care | 2025 |
| Enhance Healthcare Centenary House Ltd | Health & social care | 2015 |
| Enhance Healthcare Clyde Valley Ltd | Health & social care | 2016 |
| Enhance Healthcare Deanston Ltd | Health & social care | 2016 |
| Enhance Healthcare Greencross Ltd | Health & social care | 2025 |
| Enhance Healthcare Greenhills Ltd | Health & social care | 2025 |
| Enhance Healthcare Holdings Ltd | Health & social care | 2015 |
| Enhance Healthcare Lochleven Ltd | Health & social care | 2025 |
| Enhance Healthcare Lochside Manor Ltd | Health & social care | 2016 |
| Enhance Healthcare Ltd | Health & social care | 2011 |
| Enhance Healthcare Netherton Ltd | Health & social care | 2025 |
| Enhance Healthcare Orchard House Ltd | Health & social care | 2014 |
| Enhance Healthcare Property Ltd | Real estate | 2018 |
| Enhance Healthcare Services Ltd | Other services | 2023 |
| Enhance Healthcare Southview Ltd | Health & social care | 2025 |
| Enhance Living Support Services Ltd | Health & social care | 2015 |
| Enhance Properties (Coatbridge) Ltd | Real estate | 2021 |
| Enhance Properties (Crossford) Limited | Other services | 2022 |
| Enhance Properties Symington Limited | Other services | 2022 |
| ENP Industrial Supplies Limited | Wholesale & retail | 2018 |
| Enterprise Asset Management Ltd | Real estate | 2018 |
| Entropii Ltd | Arts & recreation | 2019 |
| Entrust Environmental Holdings Limited | Financial services | 2017 |
| Entrust Environmental Limited | Professional & scientific | 2010 |
| Envirocycle Glasgow Ltd | Transport & logistics | 2025 |
| Envirohaz Circular Waste Limited | Water & waste | 2020 |
| Envision Coaching (Scotland) Ltd | Arts & recreation | 2025 |
| Ephod Essence Ltd | Financial services | 2025 |
| Epicurean Horizons Ltd | Hospitality | 2024 |
| Epione Services Limited | Wholesale & retail | 2017 |
| Equine Minds Therapy Horses CIC | Agriculture & fishing | 2026 |
| Eras Properties Limited | Real estate | 2023 |
| ERB Property Services Ltd | Households | 2018 |
| Eriaf Ltd | Information & communication | 2026 |
| Erla Ltd | Real estate | 2024 |
| ERR Services Ltd | Wholesale & retail | 2024 |
| Ersatz (Cumbernauld) Ltd. | Other services | 2011 |
| Escapes Alba Ltd | Hospitality | 2026 |
| Eshan Medical Services Ltd | Health & social care | 2021 |
| Eshmaan Medical Services Ltd | Health & social care | 2025 |
| ESP Music Rentals Limited | Administrative & support | 2008 |
| ESP Property (Scotland) Ltd | Real estate | 2022 |
| Esper Lane Limited | Wholesale & retail | 2025 |