| Dortex (Scotland) Ltd. | Construction | 2008 |
| Doublard Investment Company Limited | | 1996 |
| Douglas Bell (Alloa) Limited | Information & communication | 2016 |
| Douglas Park Limited | Wholesale & retail | 1985 |
| Douglas Systems Ltd | Manufacturing | 2013 |
| DPR Create Ltd | Professional & scientific | 2016 |
| DR & KC News Agents Limited | Information & communication | 2020 |
| DR Clarence LIU Ltd | Health & social care | 2011 |
| DR Dilan Operations Ltd | Health & social care | 2021 |
| DR Exterior Cleaning Ltd | Administrative & support | 2026 |
| DR Lauren Medical Aesthetics Ltd | Health & social care | 2022 |
| DR Wali Ltd | Health & social care | 2021 |
| DR Zafar Limited | Health & social care | 2010 |
| Draco Express Limited | Transport & logistics | 2019 |
| Draco Logistics Limited | Transport & logistics | 2022 |
| Dream 32 Ltd | Health & social care | 2024 |
| Dress for Success Scotland Ltd | Wholesale & retail | 2010 |
| Dreth Ltd | Information & communication | 2026 |
| DRM Services Limited | Professional & scientific | 2018 |
| Drongan Battery Storage Limited | Construction | 2022 |
| DRP International Ltd | | 2025 |
| Drummair Ltd | Hospitality | 2018 |
| Drummond Flooring & Joinery Ltd | Construction | 2025 |
| DS Trades Contractor Ltd | Construction | 2021 |
| DS Ventilation Limited | Construction | 2023 |
| Dsear Standard Inspections Ltd | Professional & scientific | 2019 |
| DSM Reinstatement Ltd | Construction | 2026 |
| DSM5 Ltd | Real estate | 2025 |
| DUA MK Ltd | Hospitality | 2026 |
| Dubtree Vibes Ltd | Arts & recreation | 2018 |
| Dukudropmarket Ltd | Wholesale & retail | 2023 |
| Dulce & Sabroso Ltd | Hospitality | 2017 |
| Dump IT (Scotland) Ltd | Water & waste | 2015 |
| Dunbar Property Holdings Ltd | Real estate | 2026 |
| Dundee Audio Visual Ltd | Wholesale & retail | 2026 |
| Dunvegan Properties UK Limited | Real estate | 2003 |
| DW Sloan Limited | Construction | 2015 |
| DWA Video Engineers Ltd | Arts & recreation | 2020 |
| Dyce Battery Storage Limited | Construction | 2021 |
| Dylan 88 Ltd | Real estate | 2018 |
| Dynamic Barbering Ltd | Other services | 2022 |
| Dynamic Food Solutions Ltd | Manufacturing | 2024 |
| Dynamic Freight Solutions Ltd | Transport & logistics | 2024 |
| Dynasty DS Ltd | Wholesale & retail | 2025 |
| DYO Design Limited | Professional & scientific | 2025 |
| Dyslexic Support Limited | Education | 2025 |
| E Duncan Optometry Ltd | Health & social care | 2026 |
| E H Precision Limited | Manufacturing | 2024 |
| E HAY & Sons (Hamilton) Limited | Wholesale & retail | 2015 |
| E.K.C. Plumbing & Heating Ltd | Construction | 2008 |
| Eagle EYE Estates Ltd | Real estate | 2018 |
| Eagle P Service Limited | Wholesale & retail | 2022 |
| EAO Property Group Ltd | Financial services | 2026 |
| Earl's Barton Dental Limited | Extraterritorial | 2014 |
| Earl's Rest Holdings Ltd | Health & social care | 2025 |
| Earnock Builders Extensions Limited | Construction | 2025 |
| Earnock Extensions Limited | Construction | 2026 |
| Earnock Limited | Administrative & support | 2017 |
| EAS Contracts Limited | Professional & scientific | 2016 |
| Easi Self Storage Ltd | Transport & logistics | 2024 |
| East END Supplements Ltd | Wholesale & retail | 2025 |
| East Kilbride Private Hire Limited | Professional & scientific | 2008 |
| East Midlands Marquees Ltd | Other services | 2019 |
| Eastfield Agri Services Ltd | Transport & logistics | 2025 |
| Eastfield Development Co. Ltd. | Administrative & support | 1999 |
| Easy Shop Quarry Street Limited | Wholesale & retail | 2025 |
| Easydiagnostics UK Limited | Wholesale & retail | 2013 |
| ECC Property Maintenance Ltd | Other services | 2023 |
| Eco Clear One Limited | Construction | 2022 |
| Eco Laundrette Ltd | Other services | 2018 |
| Eco Property Portfolio Limited | Real estate | 2014 |
| Eco Scotland Holdings Ltd | Financial services | 2024 |
| Eco Scotland Trading Ltd | Hospitality | 2025 |
| Eco Sphere Technology Ltd | Manufacturing | 2021 |
| Eco Vector Ltd | Professional & scientific | 2024 |
| Eco-Sparx Limited | Other services | 2023 |
| Ecocel 2 Ltd | Energy | 2015 |
| Ecocel Ltd. | Energy | 2009 |
| Ecomi Limited | Wholesale & retail | 2024 |
| Ecosse European Haulage Ltd | Transport & logistics | 2017 |
| Ecosse Self Storage Ltd | Transport & logistics | 2026 |
| Ecotec Pro-Com Ltd | Professional & scientific | 2025 |
| Ecotec Water Limited | Professional & scientific | 2009 |
| EDC Construction Services Limited | Professional & scientific | 2019 |
| Edcm Ltd | Professional & scientific | 2016 |
| Eddlewood K9’S Ltd | Agriculture & fishing | 2024 |
| Eden Mind Therapy Limited | Health & social care | 2026 |
| Edencrest Limited | Professional & scientific | 2003 |
| Edinburgh Comely Sushi Ltd | Hospitality | 2019 |
| Edinburgh Textiles Ltd | Manufacturing | 2021 |
| Edinburgh Windscreens Limited | Wholesale & retail | 2012 |
| Education Seed UK Limited | Education | 2020 |
| Educational Editing Limited | Administrative & support | 2015 |
| Educationalappstore Ltd | Information & communication | 2011 |
| Edward Toal Haulage Ltd. | Transport & logistics | 2011 |
| Efekta Rose Ltd | | 2023 |
| Effective Transport Limited | Transport & logistics | 2022 |
| Egim Ltd | Wholesale & retail | 2020 |
| EHS FM Limited | Professional & scientific | 2023 |
| EJH Consultancy Ltd | Professional & scientific | 2025 |