| Embossed Polythene Limited | Manufacturing | 1987 |
| Emerald Hummingbird Ltd | Information & communication | 2024 |
| Emma Marie Barton Care Ltd | Health & social care | 2019 |
| Empire Electrical Contractors Ltd | Construction | 2018 |
| Empowerher Africa Ltd | Other services | 2025 |
| EMT Renovations Limited | Construction | 2013 |
| Emun RBI Services Ltd | Construction | 2026 |
| EMX Central Ltd | Wholesale & retail | 2025 |
| Enal Holdings Limited | Financial services | 2020 |
| Encafe Ltd | Hospitality | 2025 |
| Encore Communications Limited | Information & communication | 2011 |
| Enerail Ltd | Professional & scientific | 2019 |
| Energetic Travel Limited | Real estate | 2024 |
| Energy Performance(Uk) Ltd | Professional & scientific | 2013 |
| Engineering Carbon Products Limited | Professional & scientific | 1965 |
| English Enamels Limited | Manufacturing | 1992 |
| Engraving Excellence Ltd | Wholesale & retail | 2013 |
| Enhanced Buildings Ltd. | Real estate | 2019 |
| Enigma Concerts Ltd | Arts & recreation | 2025 |
| Enigma Hair and Beauty Salon Limited | Other services | 2020 |
| Enki Environmental Limited | Water & waste | 2010 |
| Enki FX Ltd | Other services | 2025 |
| Enspan Innovations Ltd | Information & communication | 2019 |
| Enstone Design Limited | Construction | 2014 |
| Enterprise Data Systems UK Limited | Information & communication | 1999 |
| Enviroarm Limited | Administrative & support | 2000 |
| Environmental Climate Systems Limited | Construction | 2005 |
| Envision Training & Development Limited | Education | 2019 |
| ENZ Engineering Limited | Construction | 2024 |
| EOP (Midlands) Ltd | Administrative & support | 2024 |
| EP Assessment Group Limited | Education | 2023 |
| Epic Weddings Ltd | Information & communication | 2011 |
| EPS Environmental Limited | Manufacturing | 2003 |
| Equine Saddle Services Ltd | Wholesale & retail | 2023 |
| Equinox Strategic Consulting Ltd | Professional & scientific | 2014 |
| Equip Storage Systems Limited | Administrative & support | 2006 |
| Erinwood Limited | Construction | 2017 |
| Erodex Graphite Systems Limited | Financial services | 2012 |
| ERW Merchants Limited | Wholesale & retail | 2016 |
| Esffon Ltd | Hospitality | 2023 |
| ESM Health Ltd | Health & social care | 2022 |
| Essencia Living Limited | Households | 2024 |
| Essential Ecology Ltd | Professional & scientific | 2024 |
| Essex Ventilation Services Group Ltd | Financial services | 2026 |
| Estate Anchor Limited | Real estate | 2023 |
| Estilo Electrical Services Limited | Construction | 2010 |
| Estilo Group Limited | Extraterritorial | 2010 |
| Estilo Hvac Limited | Construction | 2010 |
| Estilo Interiors Limited | Construction | 2003 |
| Estilo Limited | Extraterritorial | 2010 |
| Estilo Mechanical Services Limited | Extraterritorial | 2010 |
| Etesian Green Holdings Limited | Other services | 2022 |
| Etesian Green Limited | Manufacturing | 2020 |
| Ethan Rotherham Ltd | Wholesale & retail | 2025 |
| Eurotime Systems Ltd | Information & communication | 1990 |
| Eurowire Containers Limited | Manufacturing | 1998 |
| Euston, DO YOU Copy? Limited | Professional & scientific | 2015 |
| Evacuwax Ltd | Health & social care | 2023 |
| Evans Plastering Limited | Construction | 2012 |
| Even Flow Distribution Ltd | Administrative & support | 2023 |
| Ever Shine Limited | Wholesale & retail | 2023 |
| Evergreen Pharmacy (Midlands) Ltd | Health & social care | 2007 |
| Everneeded Limited | Wholesale & retail | 2025 |
| Everyhome UK Limited | Wholesale & retail | 2021 |
| Evolution Special Projects Limited | Professional & scientific | 2009 |
| Evolve Developers Ltd | Construction | 2024 |
| EVS Holdings Ltd | Financial services | 2020 |
| EVS Trust Ltd | Financial services | 2026 |
| Evtek Construction Solutions Limited | Construction | 2018 |
| Expert Leasing Limited | Administrative & support | 2013 |
| Extec Mobile Crushers & Screens Limited | Extraterritorial | 2003 |
| Exterior Cleaning and Restoration Ltd | Construction | 2013 |
| Extrablend Limited | Real estate | 2009 |
| Extreme Tech Ltd | Wholesale & retail | 2024 |
| F & P Plumbing & Heating Limited | Construction | 2013 |
| F P C (UK) Limited | Manufacturing | 1996 |
| F.R.A.O. Limited | Health & social care | 2013 |
| F.W.Watkins & Sons Limited | Manufacturing | 1933 |
| Fabwell Ltd | Manufacturing | 2003 |
| Face Envy Aesthetics Limited | Health & social care | 2021 |
| Factory Plant Associates Limited | Transport & logistics | 2001 |
| Factory Plant Removals Limited | Extraterritorial | 2011 |
| Fadewell Services Limited | Extraterritorial | 2014 |
| Fahad Mohammed 2003 Ltd | Construction | 2025 |
| Fair Homes Supported Accommodation Ltd | Hospitality | 2018 |
| Fairmile Trading Limited | Wholesale & retail | 2018 |
| Fairsphere Events Ltd | Hospitality | 2026 |
| Falcon Mobility Ltd | Wholesale & retail | 2024 |
| Falcon Retail Stations Limited | Wholesale & retail | 2026 |
| Falcon UK Housing Ltd | Real estate | 2021 |
| Falcontec Limited | Extraterritorial | 2013 |
| Falmouth Subsea Global Solutions Ltd | Transport & logistics | 2022 |
| Family Care Homeopathy Ltd | Health & social care | 2020 |
| Family First Estate Planning Ltd | Professional & scientific | 2026 |
| Farm 2 Family Meats Limited | Wholesale & retail | 2020 |
| Farms Direct Ltd | Wholesale & retail | 2019 |
| Farza Investment Limited | Real estate | 2018 |
| Fastenex (Industrial Fasteners) Limited | Manufacturing | 2017 |
| Fasttrack Training Ltd | Wholesale & retail | 2026 |
| Faymar Properties Limited | Construction | 1960 |