| DWL Restaurants Ltd | Hospitality | 2014 |
| DXR International Ltd | Information & communication | 2025 |
| Dyke 24 Properties Ltd | Real estate | 2019 |
| Dyslexia East Sussex Ltd | Education | 2026 |
| Dádé Cleaning Services Limited | Administrative & support | 2024 |
| E S W Property Holdings Ltd | Real estate | 2022 |
| E&P Plumbing & Heating LLP | | 2024 |
| E.K's Motor Services Limited | Wholesale & retail | 2006 |
| E.W.B. (Goldsmiths) Limited | Other services | 1980 |
| Eagle Build Property Limited | Construction | 2025 |
| Early Doors Ltd | Arts & recreation | 2021 |
| East Midlands Automotive Group Limited | Transport & logistics | 2018 |
| East of Bourne Properties Ltd | Real estate | 2017 |
| East Sussex Cardiology Partnership Limited | Health & social care | 2025 |
| East Sussex Electrical Contractors Ltd | Construction | 2021 |
| East Sussex Fencing Ltd | Other services | 2017 |
| East Sussex HOG Roast Events Catering Ltd | Hospitality | 2024 |
| East Sussex Homes Limited | Construction | 2004 |
| East Sussex Maintenance Limited | Construction | 2025 |
| East Sussex Property Management Ltd | Hospitality | 2025 |
| East Sussex Vision Support | Other services | 1940 |
| Eastbourne Bonfire Society Ltd | Arts & recreation | 2004 |
| Eastbourne Funeral Service Ltd | Professional & scientific | 2001 |
| Eastbourne Home Improvements Limited | Construction | 2020 |
| Eastbourne Sovereign Sailing Club Limited | Arts & recreation | 1972 |
| Eastbourne United AFC CIC | Arts & recreation | 2023 |
| Easthead Holdings Ltd | Financial services | 2017 |
| Eastside Newhaven Management Co Limited | Real estate | 2018 |
| ECC Eyecare Ltd | Wholesale & retail | 2017 |
| Eccles & Co. Restaurants Limited | Hospitality | 2025 |
| Echoplex Ltd | Information & communication | 2013 |
| ECK Bookkeeping and Accounts Ltd | Professional & scientific | 2017 |
| Eco Box Storage Limited | Administrative & support | 2024 |
| Eco Design Stoves Ltd | Construction | 2026 |
| Eco Distribution Limited | Wholesale & retail | 2009 |
| Ecobiotec Limited | Public administration | 2017 |
| Edit Sweet Ltd | Information & communication | 2018 |
| Editorial Services Direct Limited | Information & communication | 2008 |
| Edkins Millward Property Lawyers Limited | Professional & scientific | 2019 |
| Edwards Parker Ltd | Professional & scientific | 2021 |
| EGD Properties Ltd | Real estate | 2020 |
| EHB Manufacturing Ltd | Manufacturing | 2021 |
| Eixo Padel Limited | Extraterritorial | 2015 |
| Eknaa Ltd | Transport & logistics | 2022 |
| ELA Erturer Limited | Water & waste | 2021 |
| Elate Media Ltd | Information & communication | 2012 |
| Elbulle Ltd | Hospitality | 2026 |
| Eldon Timbers Ltd | Wholesale & retail | 2024 |
| Element Scaffolding Limited | Construction | 2020 |
| Elise Hair and Beauty Limited | Other services | 2017 |
| Elite Dessert Ltd | Hospitality | 2021 |
| Elite Exteriors (SE) Limited | Construction | 2015 |
| Elite Labelling Limited | Professional & scientific | 2011 |
| Elite Labelling Systems Limited | Professional & scientific | 2011 |
| Elite Lettings & Property Management Services Limited | Real estate | 2009 |
| Elite Luxury Packaging Limited | Professional & scientific | 2011 |
| Ella's Boutique Ltd. | Administrative & support | 2018 |
| Elle Synergy Ltd | Professional & scientific | 2020 |
| ELM Medical Services Ltd | Health & social care | 2026 |
| Elms & Willis Developments Limited | Real estate | 2021 |
| Elms & Willis Limited | Real estate | 2021 |
| Elphick Construction LLP | | 2012 |
| Elphick Developments Limited | Construction | 2015 |
| Elphick Holdings Ltd | Construction | 2018 |
| Else-Marie Ltd | Extraterritorial | 2022 |
| Elvis Team Ltd | Construction | 2015 |
| Emba Developments Limited | Construction | 2009 |
| Eminowicz Medical Services Limited | Health & social care | 2024 |
| Enigma Projekt Limited | Arts & recreation | 2025 |
| Enka Engineering Limited | Professional & scientific | 2015 |
| Enka Surveys Limited | Construction | 2015 |
| Enlight LED Limited | Wholesale & retail | 2023 |
| Enspire Training Limited | Professional & scientific | 2006 |
| Enterprise Medical Solutions LLP | | 2023 |
| Envi Garden Buildings Limited | Construction | 2021 |
| EO3 Limited | Professional & scientific | 2002 |
| Epayrolluk Ltd | Professional & scientific | 2014 |
| Equity Residential Limited | Real estate | 2002 |
| Eridge House Rest Home Limited | Health & social care | 2014 |
| Ersham Management Limited | Households | 2016 |
| Ersm Management Ltd | Real estate | 2023 |
| Ersm Property Solutions Limited | Real estate | 2023 |
| Escalate Generation UK Limited | Administrative & support | 2010 |
| Esker Ecolodges Limited | Hospitality | 2024 |
| EST Performance Limited | Wholesale & retail | 2013 |
| Eswitch Energy Limited | Construction | 2020 |
| ETM Associates Limited | Professional & scientific | 2004 |
| ETM Business and Training Solutions Ltd | Professional & scientific | 2021 |
| Euroduct Sheet Metal Limited | Manufacturing | 1997 |
| Evan Jolly Music Limited | Arts & recreation | 2013 |
| Evans Automotive Group Limited | Financial services | 2024 |
| Evans Surgical Limited | Health & social care | 2023 |
| Eveclo Holdings Limited | Professional & scientific | 2018 |
| Event Aptitude Ltd | Professional & scientific | 2020 |
| Events Medics Ltd | Health & social care | 2023 |
| Everett Tiles Limited | Construction | 1986 |
| Everflyht Ltd | Manufacturing | 2012 |
| Every Big Day Limited | Information & communication | 2024 |
| Evolution FX Ltd | Administrative & support | 2002 |
| Evotex Trading Limited | Wholesale & retail | 2012 |