| EW Legal Costing Limited | Professional & scientific | 2013 |
| EXCELLENCE4CHILDREN Ltd | Health & social care | 2014 |
| Excess Medical Stock Ltd | Wholesale & retail | 2021 |
| Exeter Orthopaedic Partnership LLP | | 2023 |
| Exhibition on Screen (Season 2 and 3) Ltd | Information & communication | 2014 |
| Exhibition on Screen 2017 Limited | Information & communication | 2016 |
| Exhibition on Screen Ltd | Information & communication | 2013 |
| Exigo Project Solutions Limited | Administrative & support | 2014 |
| Eximo South East Ltd | Water & waste | 2018 |
| Expert Complete Property Maintenance Ltd | Construction | 2009 |
| Expertservice Limited | Hospitality | 1998 |
| Explosive SFX Limited | Arts & recreation | 2023 |
| Express Drainage & Plumbing Ltd | Construction | 2026 |
| Extence Carpentry and Joinery Limited | Manufacturing | 2018 |
| F E Philcox Limited | Manufacturing | 2005 |
| F Ward Limited | Professional & scientific | 2025 |
| Fabulous Stays Limited | Hospitality | 2023 |
| Fairfield Plumbing & Property Ltd | Other services | 2014 |
| Fairway Construction Consultancy Ltd | Professional & scientific | 2023 |
| Fairway Electrical Contractors Ltd | Construction | 2015 |
| Falcon Cleaning and Support Services Ltd | Administrative & support | 2018 |
| Falcon Events Limited | Arts & recreation | 2023 |
| Falcon Festival Services Ltd | Administrative & support | 2023 |
| Falcon Local Limited | Administrative & support | 2024 |
| Falcon Site Equipment Services Ltd | Administrative & support | 2023 |
| Falkenvil Enterprises Ltd | Wholesale & retail | 2021 |
| Faram Limited | Real estate | 2024 |
| Farrington Windows System Limited | Professional & scientific | 2002 |
| Fastline PTY Ltd | Wholesale & retail | 2006 |
| Faulks Limited | Manufacturing | 2021 |
| FAY Bonnie Limited | Wholesale & retail | 2023 |
| FDL Assets Ltd | Financial services | 2019 |
| FE Solutions Limited | Education | 2018 |
| Feeling Developments Ltd | Construction | 2014 |
| Felix Treadwell Ltd | Arts & recreation | 2021 |
| Fellah PVT Limited | Health & social care | 2017 |
| Fenton Investments Ltd | Real estate | 2021 |
| Fernau Solutions Ltd | Professional & scientific | 2014 |
| Fernwehs Rentals Ltd | Administrative & support | 2022 |
| Fettle & Birch Limited | Other services | 2024 |
| FGJ Financial Services Ltd | Financial services | 2012 |
| FGJ Property Ltd | Real estate | 2021 |
| Fiddle Limited | Transport & logistics | 2021 |
| Field & Wing Limited | Wholesale & retail | 2026 |
| Field Groundworks Ltd | Construction | 2024 |
| Field to Fibre Limited | Arts & recreation | 2013 |
| Field to Fork Group Ltd | Hospitality | 2025 |
| Fieldskill Ltd | Administrative & support | 2004 |
| Filsell Fitness Studio Limited | Arts & recreation | 2024 |
| Finance Wise Investments Limited | Real estate | 2007 |
| Finest Floorz Ltd | Wholesale & retail | 2025 |
| Finishing Touch Home Improvements Limited | Construction | 2025 |
| Fire Safe (UK) Ltd | Public administration | 2021 |
| Firefly Technologies Limited | Other services | 2013 |
| Firefold Limited | Construction | 1984 |
| Firefold Properties Limited | Construction | 2004 |
| Firetrace Ductwork Limited | Manufacturing | 2013 |
| Firm Balls Limited | Arts & recreation | 2011 |
| First Bloc Ltd | Information & communication | 2022 |
| First in Attendance Limited | Health & social care | 2003 |
| Firststeady Limited | Construction | 1958 |
| Fishsteak Limited | Hospitality | 2008 |
| FIT4ROX Limited | Arts & recreation | 2024 |
| Fittingtime Ltd | Construction | 2013 |
| Fitzrovia AV Limited | Financial services | 2015 |
| Fizz on Foot 2025 Limited | Administrative & support | 2025 |
| Flambeau Films Limited | Information & communication | 2023 |
| Flames (Sussex) Limited | Hospitality | 2020 |
| Flaminkgo Ltd | Hospitality | 2024 |
| Flat Roofing Membranes (Southern) Ltd | Construction | 2019 |
| Flavin Property Partners Limited | Real estate | 2026 |
| Fletching Developments Ltd. | Construction | 2017 |
| Flexmill Associates Limited | Construction | 2015 |
| Flight-Assist (UK) Limited | Transport & logistics | 2010 |
| Flint Barn Estate Management Ltd | Real estate | 2018 |
| Flova UK Limited | Wholesale & retail | 2012 |
| Flowers Food ETC Ltd | Hospitality | 2025 |
| Focus Building and Development Limited | Construction | 2011 |
| Focus Design Limited | Administrative & support | 1998 |
| Fold Studio Limited | Wholesale & retail | 2023 |
| Folk & Fable HR Limited | Administrative & support | 2024 |
| Folkington Hills Limited | Administrative & support | 2025 |
| Foot Soldiers Limited | Health & social care | 2024 |
| Footballfantasies Ltd | Wholesale & retail | 2025 |
| Formex Limited | Manufacturing | 2017 |
| Fortiorfit Ltd | Wholesale & retail | 2019 |
| Fortress Fire Protection Ltd | Construction | 2021 |
| Fortune Inn Good Ltd | Hospitality | 2022 |
| Fostel Services Ltd | Professional & scientific | 2020 |
| FOX Property Services (East Sussex) Limited | Construction | 2013 |
| FOX Red Marketing Ltd | Professional & scientific | 2022 |
| Foxes Commercial Vehicle Repairs Limited | Wholesale & retail | 2012 |
| Foxtrot Tango Delta Ltd | Construction | 2021 |
| FPL Enterprises Limited | Real estate | 2019 |
| Franklinhearn Limited | Professional & scientific | 2006 |
| Fredmund Properties Limited | Real estate | 2003 |
| Freemantle Software Ltd | Information & communication | 2023 |
| French & Thorley Limited | Construction | 2003 |
| Fresh Basil Ltd | Hospitality | 2025 |
| Fresh Tracks Film Limited | Information & communication | 2026 |