| 184 CAB Company Limited | Transport & logistics | 1998 |
| A & J Clark Farming Ltd | Agriculture & fishing | 2018 |
| A C Stoddart & Sons, Colstoun (1995) | | 1996 |
| A Platform Limited | Professional & scientific | 2022 |
| A.M Autocare (Prestonpans) Ltd | Wholesale & retail | 2020 |
| A.M. Greig (Farms) Limited | Agriculture & fishing | 1996 |
| Access Dermatology Ltd | Health & social care | 2025 |
| Acredales Farm LLP | | 2017 |
| Adio Limited | Financial services | 2023 |
| Aemilia Ltd | Manufacturing | 2020 |
| AHS Gillespie Ltd | Hospitality | 2012 |
| AKP Cloud Accounting Ltd | Professional & scientific | 2023 |
| Albatross Decommissioning Limited | Construction | 2019 |
| Aletomen Limited | Professional & scientific | 2026 |
| Allkor Ltd | Professional & scientific | 2020 |
| Alternative History Haunted Tours Limited | Arts & recreation | 2025 |
| Amisfield Preservation Trust | Administrative & support | 1999 |
| Amro Development Limited | Construction | 2016 |
| Angus Steven Associates Limited | Professional & scientific | 2017 |
| Antony Harvey Executive Limited | Professional & scientific | 2025 |
| APH10 Financial Services Limited | Extraterritorial | 2016 |
| Apollo Indigo Limited | Professional & scientific | 2003 |
| Arctec Build Ltd | Professional & scientific | 2015 |
| Areacentre Limited | Construction | 1993 |
| ARJ Building and Design Services Limited | Construction | 2011 |
| Arkwright & Wills Ltd | Administrative & support | 2021 |
| Artpointm Ltd | Education | 2025 |
| Ashton Price Ltd | Professional & scientific | 2025 |
| AT Consulting (Scotland) Ltd | Professional & scientific | 2013 |
| Atlas Domes Ltd | Manufacturing | 2021 |
| Audit-Ology Ltd | Administrative & support | 2019 |
| Azure AR Limited | Information & communication | 2021 |
| B W Alba Ltd | Professional & scientific | 2022 |
| B.Alternative Ltd | Construction | 2024 |
| Bannerman Intelligence and Security Services Ltd | Administrative & support | 2025 |
| BC Change Management Consulting Limited | Professional & scientific | 2012 |
| Beechwood Ventures Limited | Professional & scientific | 1976 |
| Belgrave Homes (Scotland) Limited | Real estate | 1998 |
| Big Squid Events & Production Ltd | Professional & scientific | 2019 |
| Biggs Broadcasting Ltd. | Information & communication | 2011 |
| Bishop & Waugh Property Services Ltd | Construction | 2026 |
| BJ Slater Engineering Ltd | Professional & scientific | 2019 |
| Bleep Design Limited | Professional & scientific | 2019 |
| BM Forest & Land Ltd | Construction | 2014 |
| Bmcp Pipeline Ltd | Professional & scientific | 2015 |
| Bobby Gordon Kiltmakers Ltd. | Manufacturing | 2012 |
| Bonnington Investments (West) Limited | Other services | 2016 |
| Bonnington Investments Limited | Real estate | 2001 |
| Booth Property Management & SON Limited | Real estate | 2022 |
| Border Access Limited | Administrative & support | 2021 |
| Border Baguettes Limited | Administrative & support | 2003 |
| Border Ecosse Car Club Limited (the) | Arts & recreation | 1979 |
| Borders Risk Management Limited | Financial services | 2013 |
| Bothanna Ltd | Manufacturing | 2019 |
| Branchburg Ltd | Hospitality | 2025 |
| Brandon Investments Limited | Agriculture & fishing | 2006 |
| Braw Beard Oils Ltd | Manufacturing | 2013 |
| Breathtaking Properties Ltd | Real estate | 2025 |
| Brightline Careers Ltd | Administrative & support | 2025 |
| Brit Backcountry Ltd | Education | 2020 |
| Budget Autos Haddington Ltd | Wholesale & retail | 2026 |
| Bulgarian Cultural and Educational Centre Scotland Limited | Education | 2017 |
| Bumblebee Studios Ltd | Professional & scientific | 2026 |
| Butfield Marine Consulting Ltd | Professional & scientific | 2024 |
| Bytewolf Ltd | Information & communication | 2024 |
| C & C LOGISTICS1 Ltd | Transport & logistics | 2026 |
| C Sayers Ltd | Construction | 2025 |
| Caddam Consult Ltd | Professional & scientific | 2023 |
| Calder Road Developments Ltd | Construction | 2012 |
| Calm Waters Solution Ltd | Professional & scientific | 2014 |
| Calmart Industries Ltd | Hospitality | 2024 |
| Camcon Investments Limited | Extraterritorial | 2020 |
| Capax Consulting Ltd | Construction | 2024 |
| Capital Angels Property Fund Limited Partnership | | 2008 |
| Carberry Candles Ltd | Manufacturing | 2025 |
| Career-Ed Ltd | Administrative & support | 2020 |
| Carers of East Lothian | Health & social care | 2009 |
| Castle Moffat Properties Limited | Hospitality | 2017 |
| Castle Park Farm and Smallholdings Ltd | Agriculture & fishing | 2022 |
| Celebrations Shop Limited | Wholesale & retail | 2015 |
| Centuff Limited | Construction | 2014 |
| CF Jardine Ltd | Professional & scientific | 2024 |
| Cfind (Scotland) Ltd | Professional & scientific | 2021 |
| CGS Contracts Borders Limited | Agriculture & fishing | 2024 |
| Chancelot Consulting Limited | Information & communication | 2018 |
| Chesterman Bespoke Ltd | Manufacturing | 2023 |
| Chiara Allsup Ltd | Professional & scientific | 2023 |
| City Pads Edinburgh Limited | Professional & scientific | 2008 |
| Clarenceville Limited | Information & communication | 2025 |
| Clelland Properties Limited | Real estate | 2024 |
| Clover Care Scotland Ltd | Health & social care | 2019 |
| Cocomel Soaps Limited | Manufacturing | 2020 |
| Collaborate Creative Limited | Professional & scientific | 2014 |
| Commutedrop Ltd | Information & communication | 2025 |
| Container Central Limited | Other services | 2022 |
| Copson IT Limited | Information & communication | 2008 |
| County Physio Ltd | Health & social care | 2013 |
| CR Property Developing Ltd | Real estate | 2025 |
| Creative Link Graphic & Web Design Ltd | Professional & scientific | 2022 |
| Crerar Trustee Company 1 Limited | Other services | 2021 |