| Crerar Trustee Company 2 Limited | Other services | 2021 |
| Cross Country Electricians Limited | Construction | 2004 |
| Crossroad Consulting Limited | Financial services | 2018 |
| Cryptosupportedinburgh Ltd | Education | 2023 |
| CS Catering and Funfair Ltd | Hospitality | 2025 |
| Cubble Tech Ltd | Extraterritorial | 2023 |
| Customized Choppers LLP | | 2009 |
| D Blair & Company Limited | Other services | 2007 |
| Daddy Daycare Edinburgh Limited | Health & social care | 2014 |
| Dalkeith Leisure Limited | Hospitality | 2025 |
| Davies Properties Management Limited | Real estate | 2021 |
| Deep Structure Ltd. | Mining & quarrying | 2015 |
| Deise Engineering Limited | Construction | 2007 |
| Delchapel Limited | Professional & scientific | 1997 |
| Demq Properties Ltd | Real estate | 2023 |
| Diggory's LLP | | 2023 |
| Digital Onset Ltd | Information & communication | 2014 |
| DJC Building Services Limited | Construction | 2016 |
| Dods of Haddington Limited | Agriculture & fishing | 1974 |
| Dogs R US (Scotland) Limited | Agriculture & fishing | 2014 |
| Dovetail Health Limited | Health & social care | 2022 |
| Dowbrae Limited | Professional & scientific | 2017 |
| Down to Earth Gardening Ltd | Other services | 2011 |
| DPF Duncan Limited | Hospitality | 2021 |
| Drift 2 Limited | Hospitality | 2018 |
| Drinkwhisky Ltd | Information & communication | 2013 |
| DRIVE4LIFE Ltd | Information & communication | 2013 |
| Druid Plantscapes Ltd | Wholesale & retail | 2024 |
| Dshm Property Ltd | Construction | 2025 |
| Dunpender Properties Limited | Other services | 2024 |
| East Caledonia Properties Ltd | Real estate | 2023 |
| East Coast Masonry Ltd | Construction | 2023 |
| East Coast Vdubz Limited | Wholesale & retail | 2025 |
| East Lothian Advice Consortium Limited | Information & communication | 2015 |
| East Lothian Buses Ltd | Transport & logistics | 2017 |
| East Lothian Care Ltd | Health & social care | 2023 |
| East Lothian Food and Drink Ltd | Administrative & support | 2016 |
| East Lothian Investments Limited | Financial services | 1998 |
| East Lothian Joinery Services Ltd | Construction | 2025 |
| East Lothian Land Limited | Construction | 2000 |
| East Lothian Leisure Ltd | Arts & recreation | 2013 |
| East Lothian Mid-Market Homes LLP | | 2019 |
| East Lothian Mums Club CIC | Administrative & support | 2026 |
| East Lothian Property Ltd. | Real estate | 2019 |
| Ecohuman Escapes Ltd | Hospitality | 2021 |
| Ecohuman Ltd | Agriculture & fishing | 2018 |
| Edinburgh and London Limited | Financial services | 2018 |
| Edinburgh Apartments Ltd | Real estate | 1995 |
| Edinburgh Drone Company Ltd | Professional & scientific | 2018 |
| Edinburgh Property Consultancy Ltd. | Real estate | 2019 |
| Edinburgh Property Removals Ltd | Water & waste | 2026 |
| Edinburgh Risk Management (General) Limited | Financial services | 2001 |
| Edinburgh Risk Management Limited | Financial services | 2013 |
| Eildon Pharmacy Ltd | Wholesale & retail | 2017 |
| Eileen Hall Studio Ltd | Professional & scientific | 2024 |
| EJM Property Holdings Ltd | Real estate | 2017 |
| Elite Worldchoice Travel Agency Ltd | Administrative & support | 2010 |
| Ella and the Lion Ltd | Real estate | 2025 |
| Ellerton Properties (Scotland) Limited | Real estate | 2002 |
| ELM Edinburgh Limited | Professional & scientific | 2014 |
| Elsoms Trees Limited | Agriculture & fishing | 2020 |
| Elysian Safety 365 Ltd | Professional & scientific | 2025 |
| Emily Hogarth Ltd | Arts & recreation | 2012 |
| Enea Pictures Ltd | Information & communication | 2024 |
| Ergon Equine Limited | Manufacturing | 2008 |
| Eric LOW Consulting Ltd | Administrative & support | 2017 |
| Essence of Health Ltd | Manufacturing | 2020 |
| Euan Myles Photography Limited | Other services | 2010 |
| Evar Property Limited | Real estate | 2023 |
| Exponential Thread Limited | Professional & scientific | 2023 |
| Fairbairn House Limited | Real estate | 2022 |
| Fairway Grass and Groundcare Ltd | Other services | 2018 |
| Falko (Konditormeister) Limited | Manufacturing | 2008 |
| Fall Factor Zero Limited | Mining & quarrying | 2004 |
| Ferneylea Sporting Ltd | Arts & recreation | 2021 |
| Fidra Lettings Ltd | Real estate | 2014 |
| Findlay's Restaurant (Scotland) Ltd. | Hospitality | 2017 |
| Findlays Hotel Ltd | Hospitality | 2025 |
| Finn Higgins Joinery Ltd | Construction | 2024 |
| Fiona Mathers Ltd | Health & social care | 2026 |
| First Choice Roof Repairs Ltd | Construction | 2024 |
| First Principle Design Ltd | Professional & scientific | 2017 |
| Fiscally Limited | Professional & scientific | 2024 |
| Florabank Home Limited | Health & social care | 1996 |
| Flying Scotsman Mechanics Limited | Other services | 2019 |
| Foraging Goat Ltd | Manufacturing | 2021 |
| Fordell Farms Limited | Agriculture & fishing | 1967 |
| Forever Space Limited | Wholesale & retail | 2015 |
| Forth Building and Development Ltd | Construction | 2018 |
| Forum Interactive Consultants Limited | Professional & scientific | 2016 |
| FOX & Grace Limited | Arts & recreation | 2021 |
| Francis Campbell Limited | Wholesale & retail | 2007 |
| Fraser Mckinlay Consulting Limited | Professional & scientific | 2021 |
| Fred's Backyard Barbecue Limited | Hospitality | 2024 |
| FSA Electrical Services Limited | Construction | 2026 |
| Full Shandy Ltd | Wholesale & retail | 2025 |
| Future Sales Factory Limited | Professional & scientific | 2011 |
| G Black Builder Ltd | Construction | 2022 |
| Gallo Property Holdings Ltd | Real estate | 2026 |
| Garden Stirling Burnet Solicitors Limited | Professional & scientific | 2015 |