| Tibbs Electrical Limited | Construction | 2007 |
| TIM Smith Ltd | Construction | 2003 |
| Tivoli Place Management Company (Cheltenham) Limited | Households | 1993 |
| Tony Butler Limited | Agriculture & fishing | 2005 |
| Tootechnical Limited | Professional & scientific | 1999 |
| Top Banana Antiques Mall Limited | Transport & logistics | 1999 |
| Tortworth Estate Limited | Agriculture & fishing | 1933 |
| Total Properties Limited | Real estate | 1997 |
| Transtel Communications Limited | Information & communication | 1986 |
| Trase (UK) Limited | Information & communication | 1996 |
| Treasure Train Limited | Transport & logistics | 1993 |
| Tree Shop Limited | Agriculture & fishing | 2010 |
| Treehugger Consulting Limited | Professional & scientific | 2011 |
| Truhouse Ltd | Construction | 2021 |
| TT Electronics Fairford Limited | Manufacturing | 1978 |
| Turfit UK Limited | Administrative & support | 2003 |
| Tutex Limited | Construction | 2007 |
| Twenty Two Lansdown Crescent Management Limited | Households | 1987 |
| Two Berkeley Street Management Company Limited | Households | 1980 |
| Tyndale Vets Limited | Professional & scientific | 2010 |
| Tyten Limited | | 1980 |
| Unique Platforms Limited | Information & communication | 2022 |
| Universal Traders Gloucester Limited | Manufacturing | 2003 |
| Upton Security Limited | Information & communication | 2010 |
| V&S Healthcare Limited | Health & social care | 2015 |
| Vittoria Properties Limited | Financial services | 2002 |
| VJM Design House Limited | Professional & scientific | 2004 |
| VWS Lift Consultants Ltd | Other services | 2005 |
| Wallbridge House Management Company Limited | Households | 1986 |
| Warlord Contract Carpets Limited | Professional & scientific | 1990 |
| Warners of Gloucester Limited | Wholesale & retail | 1986 |
| Warners of Tewkesbury Limited | Real estate | 1983 |
| Warners Retail (South West) Limited | Wholesale & retail | 2018 |
| Warners Retail Group Limited | Professional & scientific | 2018 |
| Warners Retail Limited | Extraterritorial | 2018 |
| Warners Trust PLC | Professional & scientific | 1946 |
| Waterberg Welfare Society Trust | Health & social care | 2006 |
| Watmore Limited | Manufacturing | 2006 |
| Watts of Lydney Limited | Extraterritorial | 1959 |
| Watts Tyre & Rubber Company Limited | Extraterritorial | 1953 |
| Watts Urethane Products Limited | Manufacturing | 1955 |
| WE DO DO IT Limited | Information & communication | 2006 |
| Welland Camping & Fairs Limited | Arts & recreation | 2003 |
| Wellington Park Properties Limited | Real estate | 1984 |
| Wetland Environment Trust | Other services | 2007 |
| Wheelmasters (UK) Limited | Manufacturing | 1990 |
| Wild Garlic Limited | Hospitality | 2007 |
| Wildfowl & Wetlands Trust (Natural Capital) Limited | Extraterritorial | 2022 |
| Wildfowl and Wetlands Trust (Trading) Limited | Wholesale & retail | 1990 |
| William English Limited | Wholesale & retail | 2003 |
| William Mann Limited | Wholesale & retail | 1988 |
| William Morrison (Land & Property) Limited | Real estate | 2007 |
| William Welch Limited | Extraterritorial | 2005 |
| Willow Lettings Ltd | Real estate | 2021 |
| Wilmot & Co Solicitors LLP | | 2003 |
| Wilson Associates (Consulting) Limited | Professional & scientific | 2007 |
| Wiltshire Kitchens Ltd | Professional & scientific | 2003 |
| Winchcombe Scaffolding Ltd | Construction | 2003 |
| Windowcare UK Limited | Construction | 2002 |
| Winstones News Limited | Wholesale & retail | 2003 |
| Winterbotham, Strachan & Playne Limited | Extraterritorial | 1990 |
| Woodworx Joinery Limited | Manufacturing | 2004 |
| Worcester Surfacing Company Limited | Extraterritorial | 2002 |
| Workplace & Facilities Consultancy Limited | Professional & scientific | 2019 |
| Wotton Travel Limited | Administrative & support | 1985 |
| WSP Textiles Limited | Manufacturing | 2011 |
| XRE Limited | Administrative & support | 2005 |
| Yeoman Marketing Limited | Manufacturing | 1997 |
| Zeeon Ltd | Other services | 2018 |