| Satya Infotech Limited | Information & communication | 2006 |
| SBA Creations Ltd | Arts & recreation | 2021 |
| Scss Investments Limited | Real estate | 2019 |
| Sealey Holdings Ltd | Real estate | 2025 |
| Sebastien Braun Illustration Limited | Arts & recreation | 2014 |
| Sectorscope Limited | Information & communication | 1993 |
| Segrave Developments Limited | Construction | 2020 |
| Serpdrift Limited | Wholesale & retail | 2022 |
| Severn & WYE Smokery Limited | Manufacturing | 1989 |
| Sewell Mullings Limited | Professional & scientific | 1994 |
| Shane Robbins Limited | Transport & logistics | 2003 |
| Shaun Miles Roofing Limited | Construction | 2002 |
| Shiftbond Limited | Wholesale & retail | 2001 |
| Shipman Mcbryde & Co (UK) Limited | Professional & scientific | 2007 |
| Shiptinvest Limited | Professional & scientific | 1993 |
| Shooty Limited | Administrative & support | 2018 |
| Shotesham Farms Limited | Professional & scientific | 1989 |
| Siendia Ltd | Other services | 2022 |
| Silver Street Mews (Tetbury) Limited | Households | 2005 |
| Silvercrest Offshore Limited | Administrative & support | 1991 |
| Silvey Engineering Ltd | Manufacturing | 2003 |
| Silwood (Cheltenham) Management Co. Ltd. | Households | 1993 |
| Sixty Eight Hales Ltd | Real estate | 2001 |
| SJN Commercials Limited | Transport & logistics | 1994 |
| SKI Savoie Fayre Limited | Administrative & support | 1998 |
| Skogvenns Technical Consulting Ltd | Professional & scientific | 2014 |
| Slightly Foxed Limited | Information & communication | 2003 |
| Solar Information Systems Limited | Real estate | 2007 |
| Solutions & Design (Systems) Limited | Manufacturing | 2004 |
| South Gloucestershire and Stroud Academy Trust | Education | 2014 |
| Spaniel in the Works Theatre Company Limited | Arts & recreation | 2006 |
| Special Integrated Design Limited | Manufacturing | 2000 |
| Specialec Limited | Energy | 1992 |
| Springboard Graphics Limited | Professional & scientific | 2003 |
| Spurr Holdings Limited | Health & social care | 2024 |
| Square Seven Limited | Information & communication | 2003 |
| SRI Laxmi Narasimha Private Limited | Wholesale & retail | 2019 |
| ST Giles Traditional Weathervanes Limited | Extraterritorial | 2007 |
| ST Michael's Cornerstone Trust | Arts & recreation | 2000 |
| ST. Michael’S Convenience Stores Ltd | Wholesale & retail | 2024 |
| Stan Gough Electrical Contractors Limited | Construction | 2002 |
| Stanford Hall Farm (1994) Limited | Agriculture & fishing | 1993 |
| Stanhope House Management Limited | Households | 1999 |
| Station Storage (Charfield) Limited | Transport & logistics | 2025 |
| Staycation Holidays Limited | Administrative & support | 2015 |
| Steppes East Limited | Administrative & support | 1989 |
| Steppes Travel Ltd | Administrative & support | 1997 |
| Steve Ault Limited | Construction | 2003 |
| Steve Carpenter Limited | Construction | 2004 |
| Stonehouse Management Company NO 1 Limited | Hospitality | 1991 |
| Storage Tank Supplies Limited | Construction | 2003 |
| STORE4CARAVANS Limited | Other services | 2006 |
| Stroud Valleys Artspace Limited | Arts & recreation | 1997 |
| Stroud Valleys Project Limited | Health & social care | 1988 |
| Stuart Park Painting & Decorating Limited | Construction | 2007 |
| Studio 50 Limited | Administrative & support | 2007 |
| Supara Limited | Real estate | 1996 |
| Swallow Park Management (NO.2) Limited | Households | 1987 |
| Sydenham Villas Management Company Limited | Households | 1992 |
| Sysums for Hair Limited | Other services | 2003 |
| T T Tendayi Limited | Information & communication | 2024 |
| T W (1998) Limited | Wholesale & retail | 1998 |
| T.C.I. & Co UK Limited | Real estate | 1997 |
| T4 Property Maintenance Limited | Construction | 1995 |
| Taste of Thai Ltd | Hospitality | 2005 |
| Team Products UK Ltd | Administrative & support | 2007 |
| Teampursuits Limited | Arts & recreation | 2006 |
| Templeflight Limited | Construction | 1998 |
| THE Almanzora Group Limited | Other services | 1984 |
| THE Appliance Brokers Ltd | Wholesale & retail | 2018 |
| THE Campden and District Peelers Trust | Real estate | 2001 |
| THE Child Protection Development Trust Limited | Professional & scientific | 2006 |
| THE Cotswold Edit Limited | Wholesale & retail | 2025 |
| THE Danish Wardrobe Company Limited | Wholesale & retail | 2005 |
| THE Dean Close Foundation | Education | 2001 |
| THE Eurostore Group Limited | Professional & scientific | 2000 |
| THE Family Haven | Health & social care | 2001 |
| THE Flour Mill Ltd | Professional & scientific | 2000 |
| THE HAM and Cheese Company Limited | Wholesale & retail | 2005 |
| THE Hive Wotton Ltd | Education | 2025 |
| THE Holfords of Westonbirt Trust | Administrative & support | 2006 |
| THE King's School, Gloucester | Education | 1999 |
| THE Lansdown Villas Management Company Limited | Other services | 1993 |
| THE Locksmiths and Security Centre Limited | Construction | 2002 |
| THE Natural Grocery Store Limited | Wholesale & retail | 1997 |
| THE Orchard Burdett Close Management Company Limited | Households | 1990 |
| THE Original Reclamation Trading Co. Ltd | Wholesale & retail | 1999 |
| THE Paint IT Yourself Pottery Company (2002) Limited | Wholesale & retail | 2002 |
| THE Photo Studio (Tewkesbury) Limited | Professional & scientific | 2003 |
| THE Pixie Edit Limited | Other services | 2025 |
| THE Pony Racing Authority Limited | Arts & recreation | 2007 |
| THE Private Travel Company (UK) Limited | Extraterritorial | 2005 |
| THE Ratcliffe Foundation | Other services | 2007 |
| THE Small PUB Company Limited | Real estate | 2000 |
| THE Stanley Mill Industrial Heritage Preservation Trust | Professional & scientific | 1997 |
| THE Swan Hotel | | 1991 |
| THE Tailor of Gloucester Company Limited | Wholesale & retail | 2006 |
| THE White Hart Court Management Company Limited | Households | 1989 |
| Three Choirs Vineyards Limited | Manufacturing | 1984 |
| Three Owls Property Group Ltd | Financial services | 2023 |