| THE Square (Gloucester) Limited | Hospitality | 2021 |
| THE Strand Gloucester Management Company Limited | Households | 2002 |
| THE Success Script Ltd | Information & communication | 2025 |
| THE Sun Spa Glos Ltd | Other services | 2009 |
| THE Tea Room @ the Fairview Gardener Limited | Hospitality | 2026 |
| THE Tirion Retail Company Limited | Wholesale & retail | 2009 |
| THE Top Table Military Dinner Company Ltd | Hospitality | 2020 |
| THE Trailer Bar Ltd | Hospitality | 2024 |
| THE Trebles PUB Company Ltd | Hospitality | 2025 |
| THE Tudor Arms Limited | Hospitality | 2024 |
| THE Tutors Gloucester Limited | Education | 2023 |
| THE Tyre Group Holdings Limited | Extraterritorial | 2020 |
| THE Tyre Store Limited | Wholesale & retail | 1999 |
| THE Undercroft Winchcombe Limited | Wholesale & retail | 2024 |
| THE Uniquehorn Company Limited | Wholesale & retail | 2026 |
| THE UP in Arms PUB Limited | Hospitality | 2018 |
| THE Upton Property Group Ltd | Real estate | 2022 |
| THE Vault Gym (Gloucester) Ltd | Arts & recreation | 2020 |
| THE Vault Promotions Ltd | Construction | 2021 |
| THE Vault Wellness Hub Ltd | Other services | 2026 |
| THE Venture Community Hub Ltd | Health & social care | 2008 |
| THE Vineworks Partnership Limited | Agriculture & fishing | 2011 |
| THE Visit Gloucestershire Partnership CIC | Administrative & support | 2020 |
| THE Wafflers Gloucester Ltd | Hospitality | 2024 |
| THE Warehouse (Glos) Limited | Arts & recreation | 1993 |
| THE Watch Scholar Limited | Wholesale & retail | 2024 |
| THE Weavers Shop Limited | Wholesale & retail | 1991 |
| THE Wedding Box Shop Ltd | Wholesale & retail | 2014 |
| THE Wellness Bond Ltd | Other services | 2026 |
| THE Wharf Flood Bund Management Company Ltd | Other services | 2023 |
| THE Wharf House Company Limited | Real estate | 2003 |
| THE White Metal Bearing Company Ltd | Professional & scientific | 2026 |
| THE Whitehouse Principle Limited | Information & communication | 2025 |
| THE Widow Coach Ltd | Health & social care | 2022 |
| THE Wiggly Worm Limited | Administrative & support | 2007 |
| THE Wild Edit Ltd | Professional & scientific | 2025 |
| THE Wild Pizza Company Limited | Hospitality | 2019 |
| THE Wild Thyme Hotel Group Limited | Hospitality | 2024 |
| THE Willows Retirement Housing Company Limited | Households | 1970 |
| THE Wood Wall Panel Co. Ltd | Wholesale & retail | 2023 |
| THE Wool Loft (Nailsworth) Management Company Ltd. | Households | 1991 |
| THE Woozy PIG Limited | Hospitality | 2023 |
| The.Jigsaw CIC | Wholesale & retail | 2022 |
| Thea Solutions Ltd | Information & communication | 2014 |
| Theactiveunit Limited | Wholesale & retail | 2025 |
| Theajoapp Ltd | Information & communication | 2023 |
| Theatre DE Complicite Education Limited | Arts & recreation | 1991 |
| Thefifthcorner Limited | Wholesale & retail | 2015 |
| Themadmixers Ltd | Wholesale & retail | 2025 |
| Theoc Conservation Limited | Construction | 2024 |
| Theodore Montagu Ltd | Extraterritorial | 2023 |
| Theory Ltd | Construction | 2021 |
| Therapy Hive Ltd | Health & social care | 2023 |
| Thermal Sciences Limited | Information & communication | 1997 |
| Thesourcingsolutions Ltd | Professional & scientific | 2009 |
| Thetattoomachine Limited | Other services | 2024 |
| THE£500CARSHOP.COM Ltd | Wholesale & retail | 2019 |
| Thiltran Ltd | Transport & logistics | 2022 |
| Thinck Print Limited | Professional & scientific | 2011 |
| Thind & Thind Builders Limited | Construction | 2016 |
| Think IT OUT Ltd | Health & social care | 2019 |
| Think Lantern Limited | Professional & scientific | 2026 |
| Think Local SEO Ltd | Administrative & support | 2018 |
| Think Staffing Ltd | Administrative & support | 2011 |
| Thinxiha Ltd | Wholesale & retail | 2025 |
| Thirteenth and Seven Ltd | Professional & scientific | 2021 |
| This IS FES Limited | Education | 2026 |
| Thomas & Sons Limited | Real estate | 2021 |
| Thomas & Thomas Investments Ltd | Real estate | 2024 |
| Thomas & Thomas Property Ltd | Real estate | 2014 |
| Thomas Betts Financial Solutions Ltd | Financial services | 2022 |
| Thomas Flex Ltd | Households | 2025 |
| Thomas Home Developments Limited | Construction | 2007 |
| Thomas Tonks Installations Ltd | Construction | 2013 |
| Thomasjacob Ltd | Wholesale & retail | 2024 |
| Thompson & Thompson 1953 Ltd | Real estate | 2024 |
| Thompson & Thompson 1981 Ltd | Real estate | 2025 |
| Thompson & Thompson 1982 Ltd | Extraterritorial | 2026 |
| Thomridge Limited | Real estate | 2019 |
| Thornbrook Ventilation Ltd | Construction | 2007 |
| Thornewill Holdings Limited | Wholesale & retail | 2022 |
| Three Counties Fencing Limited | Construction | 2023 |
| Three Flowers Plus Ltd | Wholesale & retail | 2025 |
| Three Piece Joinery Limited | Construction | 2011 |
| Three R Corp UK Ltd | Wholesale & retail | 2025 |
| Three Seas Developments Limited | Real estate | 2025 |
| Three World Media Limited | Professional & scientific | 2014 |
| Threereasons.Co.Uk Marketing Limited | Professional & scientific | 2012 |
| Threeshiresroofing Ltd | Construction | 2026 |
| Threesixty Holdco 7 Limited | Financial services | 2020 |
| Threesixty Investco 7 Limited | Financial services | 2022 |
| Thrive Defence Limited | Manufacturing | 2024 |
| Thrive Elevate-Ed Limited | Education | 2025 |
| Thrive Event Solutions Limited | Construction | 2024 |
| Thrive International Construction Solutions Limited | Construction | 2024 |
| Thrive International Group Ltd | Construction | 2023 |
| Thrive Merch Limited | Wholesale & retail | 2024 |
| Thrive Military Limited | Manufacturing | 2024 |
| Thrive Modular Limited | Manufacturing | 2024 |
| Thrive Sport Solutions Limited | Manufacturing | 2024 |