| THE Leigh Flood Bund Management Company Limited | Households | 2015 |
| THE Life BUS CIC | Education | 2021 |
| THE Lifestyle Agency Limited | Professional & scientific | 2012 |
| THE Limes (Tetbury) Management Company Limited | Extraterritorial | 2025 |
| THE Limes Dental Practice Limited | Health & social care | 2012 |
| THE Lines Group Limited | Professional & scientific | 1978 |
| THE Lines Holdings Limited | Professional & scientific | 2002 |
| THE Literature Laboratory Limited | Wholesale & retail | 2023 |
| THE Little Building and Restoration Company Ltd | Construction | 2023 |
| THE Little Music Company Limited | Information & communication | 2007 |
| THE Makers Collection Limited | Other services | 2023 |
| THE Maker’S Loft Ltd | Arts & recreation | 2025 |
| THE Marketing Farm Limited | Administrative & support | 2007 |
| THE Marlborough Arms (Cirencester) Ltd | Hospitality | 2021 |
| THE Massage Company - Ealing Ltd | Health & social care | 2021 |
| THE Massage Company - Putney Ltd | Health & social care | 2021 |
| THE Mastro Bros Ltd | Hospitality | 2025 |
| THE Mcquater Partnership Ltd | Professional & scientific | 2020 |
| THE Mears Foundation | Administrative & support | 2009 |
| THE Meat Locker GL Ltd | Wholesale & retail | 2026 |
| THE Melted Cheese Co Ltd | Hospitality | 2024 |
| THE Mens Groom by Ashley Paul Limited | Other services | 2007 |
| THE Mercers Hall Gloucester Event Company Limited | Other services | 2023 |
| THE Money Coach Ltd | Education | 2026 |
| THE Mortgage and Protection Partnership Ltd | Financial services | 2013 |
| THE Moving Group Ltd | Transport & logistics | 2019 |
| THE Mukherjees PVT Limited | Health & social care | 2021 |
| THE Music Works (Charity) | Education | 2004 |
| THE National Center for Accounts & Training Limited | Wholesale & retail | 2024 |
| THE Native Hotel Company Limited | Hospitality | 2019 |
| THE NEGOTI8ORS Ltd | Extraterritorial | 2017 |
| THE Nelson Gloucester Limited | Hospitality | 2023 |
| THE Neo's Wholesale Ltd | Wholesale & retail | 2019 |
| THE Nest in Gloucestershire Community Interest Company | Manufacturing | 2023 |
| THE New Gloucester Antiques Centre Limited | Wholesale & retail | 2015 |
| THE New PUB Ltd | Hospitality | 2025 |
| THE Night Angels CIC | Wholesale & retail | 2024 |
| THE Old Chapel Trading Co Limited | Wholesale & retail | 2023 |
| THE Old Custom House Management Limited | Households | 2003 |
| THE Old DUN Horse Management Company Limited | Households | 2019 |
| THE Old Forge Inn Whitminster Ltd | Hospitality | 2025 |
| THE Old Joinery Company Limited | Manufacturing | 2010 |
| THE Old Market Place Management Company Limited | Real estate | 2015 |
| THE Online College Ltd | Education | 2020 |
| THE Openside Ltd | Hospitality | 2020 |
| THE Optimum Training Company Ltd | Wholesale & retail | 2024 |
| THE Organic Smoked Salmon Company Limited | Extraterritorial | 2025 |
| THE Oxbridge Admissions Group Limited | Education | 2024 |
| THE P & M Group Management Services Limited | Manufacturing | 2026 |
| THE Packet Company Limited | Manufacturing | 2015 |
| THE Paddocks Cottages Limited | Hospitality | 2012 |
| THE Paintworks (Gloucestershire) Ltd | Wholesale & retail | 2010 |
| THE Part and Parcel PUB Limited | Hospitality | 2021 |
| THE Patchwork Basket Limited | Wholesale & retail | 2017 |
| THE Perfectly Picked and Packed Company Limited | Extraterritorial | 2005 |
| THE Philipians Limited | Real estate | 2005 |
| THE Phones Club Ltd | Wholesale & retail | 2025 |
| THE Point Blue Network Limited | Administrative & support | 2003 |
| THE Property Plaice Ltd | Real estate | 2026 |
| THE Property Wizards Group Ltd | Real estate | 2021 |
| THE Prototype Works Limited | Professional & scientific | 2016 |
| THE Pullman Centre (Gloucester) Limited | Households | 1989 |
| THE Quality Assurance Agency for Higher Education | Education | 1997 |
| THE Radio Broadcast Co Ltd | Information & communication | 2023 |
| THE Radley Forensic Document Laboratory Limited | Professional & scientific | 2011 |
| THE Raikes Journal CIC | Information & communication | 2024 |
| THE RAW Dog Food Shop Limited | Wholesale & retail | 2023 |
| THE Rayer Group Limited | Extraterritorial | 2000 |
| THE Really Useful People Ltd | Administrative & support | 2020 |
| THE Red Hart Inn Ltd | Hospitality | 2015 |
| THE Red Lion (Arlingham) Limited | Hospitality | 2013 |
| THE Red Lion Inn at Huntley Ltd | Hospitality | 2019 |
| THE Redeemed Christian Church of GOD Christ's Love Assembly Swindon | Other services | 2019 |
| THE Regency Kitchen & Bathroom Company Limited | Construction | 2018 |
| THE Regulated Parent CIC | Other services | 2025 |
| THE Residential Surveyors Cheltenham Limited | Real estate | 2019 |
| THE Rickety Press Limited | Hospitality | 2011 |
| THE Rollright Trust Limited | Education | 1997 |
| THE RUH Collective CIC | Public administration | 2025 |
| THE Rusk Co (SA) Ltd | Manufacturing | 2021 |
| THE Rusty Bicycle Limited | Hospitality | 2011 |
| THE Safety Knife Co. Ltd. | Manufacturing | 1993 |
| THE Salon Look Limited | Wholesale & retail | 2009 |
| THE Sauna at the Cottage Ltd | Other services | 2024 |
| THE School of AI Automation Ltd | Information & communication | 2025 |
| THE Sentinel Portfolio Limited | Information & communication | 2017 |
| THE Service Centre ( Gloucester ) Limited | Wholesale & retail | 2008 |
| THE Shed Cafe Kingsholm Ltd | Hospitality | 2025 |
| THE Shutter Market Ltd | Construction | 2024 |
| THE Silk Petal Ltd | Wholesale & retail | 2025 |
| THE Sisters PUB Co Ltd | Hospitality | 2026 |
| THE Skin Clinic With Katie Ltd | Other services | 2022 |
| THE Slow & Steady PUB Limited | Hospitality | 2023 |
| THE Soldiers of Gloucestershire Museum | Arts & recreation | 2002 |
| THE Soldiers of Gloucestershire Museum Trading Limited | Wholesale & retail | 2020 |
| THE Somatic School Limited | Education | 2018 |
| THE South Gloucester Learning Trust | Education | 2010 |
| THE Southern Football League Limited | Other services | 1999 |
| THE Specialist External Cleaning Company Limited | Administrative & support | 2023 |
| THE Spring Centre Trust Fund | Health & social care | 1994 |