| Rush Hour Care Limited | Health & social care | 2025 |
| Russell Grab Hire Limited | Transport & logistics | 2019 |
| Russell Investment Group Limited | Financial services | 2020 |
| Rusty - Oak Limited | Real estate | 2013 |
| Rusty Shilling Developments Limited | Construction | 2006 |
| Ruthajwilson Ltd | Information & communication | 2014 |
| Rutherfords Skip Hire Limited | Water & waste | 2002 |
| RUX Legatum Limited | Real estate | 2024 |
| RVA Synergies Ltd | Wholesale & retail | 2010 |
| RW Consort Ltd | Professional & scientific | 2022 |
| Rxlocums Limited | Wholesale & retail | 2021 |
| Ry's FRY Ltd | Hospitality | 2019 |
| Ryan Fortey Hair Limited | Administrative & support | 2017 |
| Ryan Hogarty Ltd | Professional & scientific | 2022 |
| Ryan-Mar Limited | Real estate | 2013 |
| Ryandeepak Ltd | Health & social care | 2016 |
| Ryestreet Limited | Financial services | 2011 |
| Rykool Air Conditioning Ltd | Energy | 2021 |
| Ryman Construction Limited | Construction | 2019 |
| Ryman Holdings Limited | Financial services | 2023 |
| Ryman Property Limited | Real estate | 2023 |
| Rypple Consulting Ltd | Professional & scientific | 2024 |
| Rypro Accountants Ltd | Professional & scientific | 2023 |
| Rypro Ltd | Real estate | 2018 |
| Ryptx Ltd | Professional & scientific | 2022 |
| Röchling Fibracon Limited | Manufacturing | 1979 |
| Röchling Insoll Limited | Manufacturing | 1998 |
| S & K Car Repairs Ltd | Wholesale & retail | 2019 |
| S & M Tapers Ltd | Construction | 2022 |
| S & P Spaces Limited | Real estate | 2019 |
| S & Z Thomas Ltd | Construction | 2024 |
| S and R Partners Consulting Ltd | Professional & scientific | 2010 |
| S B Electrical Contractors Ltd | Construction | 2014 |
| S Burford Properties Ltd | Real estate | 2022 |
| S D F Limited | Wholesale & retail | 1995 |
| S Desai Consulting Limited | Administrative & support | 2012 |
| S Dooply Electrical Ltd | Construction | 2021 |
| S J Publishing Ltd | Information & communication | 2024 |
| S K MED Limited | Health & social care | 2018 |
| S M Nicklin Limited | Administrative & support | 2023 |
| S Merrett Services Limited | Arts & recreation | 2015 |
| S N Engineering Ltd | Wholesale & retail | 2007 |
| S P Ganesh Realtors Ltd | Real estate | 2025 |
| S R Electrical Contractors Ltd | Construction | 2005 |
| S Robbins Limited | Health & social care | 2019 |
| S Rosato and Sons Limited | Construction | 1993 |
| S S Mobile Mechanic Ltd | Wholesale & retail | 2025 |
| S W L Inns Ltd | Hospitality | 2012 |
| S W Tyres ( Gloucester ) Ltd | Wholesale & retail | 2026 |
| S&G 101 Ltd | Other services | 1999 |
| S&J Moulinie Landscapes Ltd | Administrative & support | 2012 |
| S&M Solutions Glos Ltd | Transport & logistics | 2016 |
| S&S Lifestyle Properties Ltd | Real estate | 2020 |
| S-Alive Limited | Wholesale & retail | 2020 |
| S.A. Gardening & Landscaping Ltd | Administrative & support | 2022 |
| S.A.F.E Plumbing & Heating Ltd | Construction | 2018 |
| S.A.H.Electrical Ltd | Construction | 2011 |
| S.C.F. Plumbers & Builders Limited | Energy | 2003 |
| S.P.A. Holdings (Gloucester) Limited | Professional & scientific | 2020 |
| S.P.A. Veterinary Services Limited | Professional & scientific | 2007 |
| S.Reeves Building & Maintenance Ltd | Construction | 2021 |
| S.S. White Manufacturing | Manufacturing | 1987 |
| S.T.A.T.T Roses Limited | Health & social care | 2013 |
| S.Ward Builders Limited | Construction | 2002 |
| S.Woodford Ltd | Real estate | 2024 |
| S.Woolacott Ltd | Construction | 2022 |
| S3D Housing Solutions Limited | Real estate | 2026 |
| S4TEL Limited | Information & communication | 2015 |
| SA Commissioning Ltd | Construction | 2025 |
| SA Glos Ltd | Hospitality | 2020 |
| SAB Chippy Ltd | Hospitality | 2026 |
| Sabholdinguk Ltd | Real estate | 2023 |
| Sablik Building Service Ltd | Construction | 2016 |
| Sabmm Limited | Wholesale & retail | 2025 |
| Sacklyn Roofing Limited | Construction | 2004 |
| Saddle Smith Ltd | Manufacturing | 2020 |
| Sade Properties Ltd | Real estate | 2014 |
| Sadra Carpentry Ltd | Construction | 2026 |
| Safar Media Ltd | Manufacturing | 2019 |
| Safe & Secure Windows & Doors Ltd | Manufacturing | 2023 |
| Safe Air Solutions Ltd | Administrative & support | 2022 |
| Safe From Risk Limited | Administrative & support | 2018 |
| Safe Hands Medical Services Ltd | Health & social care | 2022 |
| Safe Hands Multiconcept Ltd | Other services | 2023 |
| Safe Haven Support Services Limited | Transport & logistics | 2026 |
| Safe House Rental Limited | Real estate | 2024 |
| Safeguard Professional Services Ltd | Professional & scientific | 2023 |
| Safeguard Security Services (Glos.) Limited | Arts & recreation | 1989 |
| Safehands Independent Financial Advisers Ltd | Financial services | 2011 |
| Safepeople Ltd | Professional & scientific | 2025 |
| Safepreneur Ltd | Wholesale & retail | 2025 |
| Safety Check Ltd | Construction | 2021 |
| Safety Training and Assessment Services Limited | Professional & scientific | 2003 |
| Safety Works Group Limited | Extraterritorial | 2015 |
| Safetyplan Limited | Information & communication | 2019 |
| Safire Enterprises Ltd | Transport & logistics | 2025 |
| Safosk Limited | Health & social care | 2022 |
| Safpro Limited | Administrative & support | 2013 |
| Safran Electronics & Defense UK Ltd | Wholesale & retail | 2012 |
| Safran Engineering Services UK Limited | Professional & scientific | 2003 |