| London Infusions Co Limited | Manufacturing | 2023 |
| London Road Store 1 Ltd | Wholesale & retail | 2026 |
| Lone Elephant Limited | Wholesale & retail | 2020 |
| Lone Star Surveyors Ltd | Professional & scientific | 2026 |
| Lonedesigns London Ltd | Administrative & support | 2019 |
| Long Promise Catering Ltd | Manufacturing | 2024 |
| Longbrick Ltd | Real estate | 2018 |
| Longbrooke House Limited | Construction | 2020 |
| Longford Alpha Bidco 1 Ltd | Professional & scientific | 2026 |
| Longford Alpha Midco Ltd | Professional & scientific | 2026 |
| Longford Beta Ltd | Professional & scientific | 2026 |
| Longford Equity Group Ltd | Professional & scientific | 2024 |
| Longford Garage Group Ltd | Professional & scientific | 2024 |
| Longford Gate Ltd | Households | 2010 |
| Longford Plastering Limited | Construction | 2004 |
| Longford Property and Lettings Ltd | Real estate | 2023 |
| Longhope Welding Company Limited | Manufacturing | 2006 |
| Longlevens AFC Limited | Arts & recreation | 2023 |
| Longlevens Building & Roofing (Holdings) Limited | Construction | 2014 |
| Longlevens Building & Roofing Limited | Construction | 2002 |
| Longlevens Chiropractic & Sports Injury Clinic Ltd | Health & social care | 2005 |
| Longlevens Rugby Football Club Limited | Arts & recreation | 2011 |
| Longney Manor Equestrian Ltd | Agriculture & fishing | 2026 |
| Longstone Cleaning & Co. Ltd | Administrative & support | 2024 |
| Look! Lets Ltd | Real estate | 2012 |
| Looli Enterprises Limited | Real estate | 2025 |
| Loop Electrical Limited | Construction | 2016 |
| Loop Enterprise Software Limited | Information & communication | 2024 |
| Lopwert Ltd | Other services | 2022 |
| Loran (Investments) Limited | Professional & scientific | 1999 |
| Loran Services Limited | Real estate | 2000 |
| Lorford's Holdings Limited | Financial services | 2019 |
| Lorford's Limited | Manufacturing | 2019 |
| Lotus Living Spaces Ltd | Real estate | 2022 |
| Lotusv Food Ltd | Wholesale & retail | 2023 |
| Love + Play Ltd | Wholesale & retail | 2023 |
| Love and Purpose Ltd | Other services | 2015 |
| Love Film Productions Limited | Information & communication | 2008 |
| LOVE2PETS Ltd | Wholesale & retail | 2020 |
| Lovefun Ltd | Extraterritorial | 2025 |
| Lovelock Family Ltd | Education | 2021 |
| LOW Carbon Energy Developments Limited | Construction | 2023 |
| Loweconex Group Limited | Information & communication | 2017 |
| LPI Facades Ltd | Construction | 2023 |
| Lpnmedia Limited | Administrative & support | 2024 |
| LSD Transport Ltd | Transport & logistics | 2023 |
| Lucamar Ltd | Administrative & support | 2020 |
| Lucky XI Ltd | Administrative & support | 2025 |
| Lucy's Dog Walking Service Ltd | Other services | 2024 |
| Ludobloom Limited | Wholesale & retail | 2023 |
| Ludonautics Limited | Professional & scientific | 2023 |
| Luigi’S Baps Ltd | Hospitality | 2025 |
| Luke Crunden Fitness Ltd | Education | 2023 |
| Lulworthco Prop 1 Limited | Real estate | 2022 |
| Lumiere Architects Ltd | Professional & scientific | 2024 |
| Lumiparent Ltd | Wholesale & retail | 2020 |
| Lumo Lenses Ltd | Wholesale & retail | 2025 |
| Lunartec Ltd | Information & communication | 2026 |
| Lusanda Wasa Ltd | Health & social care | 2025 |
| Luscher UK Limited | Information & communication | 1995 |
| Lutra Housing Ltd | Households | 2024 |
| Luxe Laundry Rooms Ltd | Other services | 2026 |
| Luxe Property Stays Ltd | Hospitality | 2025 |
| Luxe Tones Ltd | Wholesale & retail | 2020 |
| Luxury Boutique Lettings Limited | Hospitality | 2020 |
| Luxury Homes Gloucester Ltd | Real estate | 2019 |
| Luxury Lawns (Gloucester) Ltd. | Administrative & support | 2018 |
| Luxury Outdoor Getaways Ltd | Agriculture & fishing | 2020 |
| Luxury Pavilions Marquee Hire Limited | Arts & recreation | 2005 |
| Luxx PR Limited | Professional & scientific | 2014 |
| Lvia Ltd | Professional & scientific | 2018 |
| LWC Precision Engineering Ltd | Professional & scientific | 2018 |
| LXF Nation Ltd | Other services | 2021 |
| Lycett, Browne-Swinburne & Douglass Limited | Financial services | 1961 |
| Lycetts Financial Services Limited | Financial services | 1986 |
| Lycetts Holdings Limited | Professional & scientific | 2006 |
| Lycetts Risk Management Services Limited | Financial services | 2017 |
| Lydbrook & District Community Social Club Limited | Hospitality | 2004 |
| Lyle Consultancy Services Limited | Professional & scientific | 2009 |
| Lyme Regis Stays Limited | Real estate | 2022 |
| Lyndon Creswick Heating & Plumbing Limited | Construction | 2012 |
| M & D Group (South West) Ltd | Extraterritorial | 2014 |
| M & D Welding (Gloucester) Limited | Manufacturing | 2005 |
| M & G Properties (Holdings) Limited | Financial services | 2006 |
| M & K Rees Limited | Professional & scientific | 2010 |
| M & MG Properties Limited | Real estate | 2026 |
| M & N Accountancy Ltd | Professional & scientific | 2017 |
| M & N Haulage Limited | Transport & logistics | 2014 |
| M & V Prokopiou Limited | Hospitality | 2009 |
| M B Barber Shop Ltd | Other services | 2026 |
| M B Electrical Contracts Ltd | Construction | 2015 |
| M B Holder and Sons Limited | Wholesale & retail | 2003 |
| M Bourne Holdings Ltd | Professional & scientific | 2018 |
| M C Travel Management Limited | Administrative & support | 2002 |
| M Coates Industrial Engineers Limited | Manufacturing | 2003 |
| M Dean Building Ltd | Construction | 2025 |
| M Dyer Services Limited | Wholesale & retail | 2017 |
| M F Ducting Ltd | Energy | 2015 |
| M F Management Services Ltd | Administrative & support | 2025 |
| M Giles 365 Secure Ltd | Construction | 2021 |