| Liberas Solutions Ltd | Information & communication | 2014 |
| Liberty Athletic Limited | Wholesale & retail | 2019 |
| Libretto Ltd | Professional & scientific | 2023 |
| Libuc Ltd | Professional & scientific | 2023 |
| Life After Life Ltd | Other services | 2022 |
| Life Secure Limited | Administrative & support | 2025 |
| Lifely Surveyors Limited | Other services | 2007 |
| Lift AOG Aeroparts Ltd | Transport & logistics | 2025 |
| Light in the Dark CIC | Health & social care | 2025 |
| Light Realm Ltd | Manufacturing | 2022 |
| Lightcross Property Management Company Limited | Households | 1991 |
| Lighthouse Building Projects Ltd | Construction | 2026 |
| Lightning Mcclean Limited | Administrative & support | 2025 |
| Like Holdings Ltd | Information & communication | 2018 |
| Like Radio Ltd | Information & communication | 2015 |
| Like Team Limited | Professional & scientific | 2020 |
| Lillybear Limited | Real estate | 2026 |
| Lillywhite Facility Solutions Ltd | Administrative & support | 2025 |
| Lillywhite Limited | Professional & scientific | 2003 |
| Lily P’S Beauty Boutique Ltd | Other services | 2023 |
| Lily's Restaurant & Tearooms Limited | Hospitality | 2020 |
| Limbird Limited | Construction | 1974 |
| Lime Court (Churchdown) Management Limited | Households | 2011 |
| Lime Masonry Ltd | Construction | 2015 |
| Limetastic Ltd | Wholesale & retail | 2025 |
| Linden Barber Shop Ltd | Other services | 2025 |
| Linden Carpentry Limited | Manufacturing | 2021 |
| Line Contracts Ltd | Information & communication | 1997 |
| Linear Fencing Ltd | Construction | 2023 |
| Lingua (UK) Limited | Education | 2002 |
| Linguafog Ltd | Information & communication | 2025 |
| Link Ecology Limited | Professional & scientific | 2014 |
| Link Self Build Limited | Construction | 2013 |
| Lion Contracting Services Ltd. | Information & communication | 2022 |
| Lionbooks Ltd | Professional & scientific | 2019 |
| Liquid Control Systems Limited | Manufacturing | 2017 |
| Lisa Burdus Limited | Administrative & support | 2020 |
| Listening Post Counselling Service Limited | Health & social care | 1991 |
| Listory Ltd | Wholesale & retail | 2026 |
| Litchfield Car Sales Limited | Extraterritorial | 2019 |
| Litchfield Electric Limited | Extraterritorial | 2019 |
| Litchfield EV Limited | Extraterritorial | 2019 |
| Litchfield Imports Limited | Extraterritorial | 2019 |
| Litchfield Motors Limited | Wholesale & retail | 2002 |
| Litchfield Motorsport Limited | Extraterritorial | 2019 |
| Litchfield Paint Protection Limited | Extraterritorial | 2019 |
| Litchfield Performance Limited | Extraterritorial | 2019 |
| Litchfield Storage Limited | Real estate | 2024 |
| Little B Services Limited | Administrative & support | 2015 |
| Little Monkeys Childcare (Gloucester) Limited | Health & social care | 2021 |
| Little Monsters Services Ltd | Administrative & support | 2024 |
| Little Tiddlywinks Ltd | Wholesale & retail | 2012 |
| Little White Van MAN Ltd | Extraterritorial | 2017 |
| Littlebird Driving School Ltd | Education | 2025 |
| Littlepawspetcremationgloucestershire Ltd | Other services | 2025 |
| Livall Logistics Limited | Transport & logistics | 2014 |
| Livanova UK Limited | Manufacturing | 2004 |
| Live 9 Lives Limited | Wholesale & retail | 2025 |
| Livelane Products Ltd | Wholesale & retail | 2016 |
| Livewire Electrical Design and Installation Ltd | Construction | 2009 |
| Living Stone Property Solutions Ltd | Real estate | 2020 |
| Living With the Lions Sports Travel Limited | Administrative & support | 2000 |
| Livingvibe Limited | Wholesale & retail | 2025 |
| LIZ Wright Ministries Ltd | Information & communication | 2016 |
| LJ Concepts Limited | Professional & scientific | 2003 |
| LJB Motors Ltd | Wholesale & retail | 2023 |
| LJD Music Services Limited | Professional & scientific | 2025 |
| LJP Global Ltd | Wholesale & retail | 2026 |
| Lj’S Residential Ltd | Real estate | 2023 |
| LLA Body Modification Ltd | Arts & recreation | 2025 |
| Lloyd Sharp Restaurants Limited | Hospitality | 2017 |
| Lloyds Luxury Living Ltd | Real estate | 2024 |
| LLR Roofing Ltd | Construction | 2023 |
| LM A&R Ltd | Construction | 2016 |
| LM Recruitment Solutions Ltd | Administrative & support | 2023 |
| LMC Bookkeeping Ltd | Professional & scientific | 2021 |
| LMS Freshwater Limited | Information & communication | 2019 |
| LNH Management Solutions Limited | Professional & scientific | 2012 |
| Loam Productions Ltd | Information & communication | 2022 |
| Loba Enterprises Limited | Wholesale & retail | 2023 |
| Lock Warehouse Management Company Ltd | Real estate | 2024 |
| Lock'In Gym Ltd | Arts & recreation | 2025 |
| Lock-Down Construction & Safety Services Ltd | Construction | 2020 |
| Lockdown Bakers Ltd | Hospitality | 2020 |
| Lockedinnelite Limited | Wholesale & retail | 2025 |
| Lockelec Ltd | Construction | 2021 |
| Locksmiths Gloucester Limited | Administrative & support | 2022 |
| Loco Motives Limited | Manufacturing | 2015 |
| Locutus EST Terra Limited | Real estate | 2016 |
| LOG Ltd | Transport & logistics | 2020 |
| Logical Endeavour Solutions Limited | Information & communication | 2016 |
| Logik Recruitment Limited | Agriculture & fishing | 2009 |
| Logikal TF Ltd | Wholesale & retail | 2021 |
| Logistics Options Ltd | Transport & logistics | 2023 |
| Loladex 3P Limited | Manufacturing | 2024 |
| Loladex P3 Limited | Information & communication | 2023 |
| Lolus Couture African Crafts Ltd | Manufacturing | 2025 |
| London Construction Alliance Limited | Construction | 2018 |
| London Construction Group Limited | Construction | 2017 |
| London Construction Logistics Limited | Extraterritorial | 2016 |