| FIRST4COMS Ltd | Information & communication | 2003 |
| FIRST4HOMECARE Ltd | Health & social care | 2016 |
| Firsteng Ltd | Professional & scientific | 2018 |
| Fish & Seafood Limited | Wholesale & retail | 1975 |
| Fish Master 2026 Ltd | Hospitality | 2026 |
| Fishers Place Management Ltd | Households | 2000 |
| Fishpond World Limited | Wholesale & retail | 2011 |
| Fit for Life Gloucestershire Ltd | Arts & recreation | 2026 |
| Fit to Explore Limited | Education | 2020 |
| Fitness Automations Ltd | Information & communication | 2025 |
| Fitness Megastore Ltd | Wholesale & retail | 2021 |
| Fitrovert Limited | Information & communication | 2023 |
| Fitss Limited | Information & communication | 2017 |
| Five Star (Gloucestershire) Ltd | Transport & logistics | 2022 |
| Five Star / First Associated Transport Limited | Transport & logistics | 2013 |
| Five Valleys Removals Limited | Administrative & support | 2002 |
| Five Valleys Systems Ltd | Information & communication | 2017 |
| Fives & 20'S Limited | Wholesale & retail | 2026 |
| Fix-It Property Care Gloucester Ltd | Construction | 2026 |
| Fixat 4 U Ltd | Transport & logistics | 2022 |
| Fixing Point Limited | Wholesale & retail | 1986 |
| Fixing Point Properties Limited | Real estate | 2023 |
| FJP Solutions Ltd | Construction | 2026 |
| Flamefighter Fire Protection Ltd | Public administration | 2011 |
| Flat CAP Roasters Limited | Wholesale & retail | 2017 |
| Flavashack Ltd | Wholesale & retail | 2023 |
| Fleck Ltd | Arts & recreation | 2024 |
| Fleet Electric Leasing Limited | Administrative & support | 2020 |
| Fleet Gloucester Limited | Professional & scientific | 2006 |
| Fleet Law Limited | Professional & scientific | 2026 |
| Fleet Solicitors LLP | | 2004 |
| Fleetwood FOX Limited | Manufacturing | 2003 |
| Fleming Properties (Gloucester) Limited | Financial services | 2025 |
| Flexicare Homeservices Stroud Ltd | Health & social care | 2017 |
| Flexicare Homeservices UK Limited | Health & social care | 2007 |
| Flexicare Newent and Ledbury Ltd | Health & social care | 2023 |
| Flexton Digital Ltd | Information & communication | 2016 |
| FLH Waste Solutions Ltd | Water & waste | 2022 |
| Flickr & Foam Ltd | Wholesale & retail | 2026 |
| Flight Efficiency Limited | Information & communication | 2013 |
| Flightgate Ltd | Construction | 2020 |
| Flipick Limited | Information & communication | 2014 |
| FLO Services Ltd | Transport & logistics | 2025 |
| Flo-Vent Limited | Construction | 2018 |
| Flolec Electrical Limited | Construction | 2019 |
| Florence Maple Ltd | Real estate | 2023 |
| Florentia Flowers Limited | Arts & recreation | 2017 |
| Florenus Limited | Wholesale & retail | 2025 |
| Florenze Properties Ltd | Construction | 2026 |
| Flotein Ltd | Wholesale & retail | 2025 |
| Flow Motion Pictures Ltd | Information & communication | 2015 |
| Flow State Jiu-Jitsu Ltd | Education | 2025 |
| Flowtech Water Solutions Holdings Limited | Financial services | 2017 |
| Flowtech Water Solutions Limited | Manufacturing | 2004 |
| Fluffy Projects Ltd | Professional & scientific | 2024 |
| Fluid Mechanics Ltd | Professional & scientific | 1997 |
| Fluma Studio Ltd. | Wholesale & retail | 2025 |
| Fluxcast Ltd | Information & communication | 2013 |
| Fluxvending Ltd | Wholesale & retail | 2025 |
| FLY Move Transport Ltd | Wholesale & retail | 2021 |
| Flyup Ltd | Transport & logistics | 2010 |
| FM Tree Services Ltd | Agriculture & fishing | 2026 |
| FNL Architectural Consultancy Ltd | Professional & scientific | 2012 |
| Foliation Limited | Administrative & support | 2002 |
| Folley Electrical Contractors Ltd | Construction | 2014 |
| Folley Property Ltd | Real estate | 2020 |
| Follis Healthcare Limited | Health & social care | 2015 |
| Fondtron Limited | Extraterritorial | 2024 |
| Fone Square Limited | Wholesale & retail | 2021 |
| Fonesmart Limited | Wholesale & retail | 2017 |
| Food Equipment Europe Limited | Wholesale & retail | 1999 |
| Foodshire Ltd | Hospitality | 2025 |
| Football Away Days Ltd | Administrative & support | 2025 |
| Football Heritage Tours Ltd | Administrative & support | 2024 |
| Footybet Limited | Arts & recreation | 2026 |
| FOR the Child Ltd | Education | 2021 |
| FOR Wills Limited | Professional & scientific | 2016 |
| Fords Garden Buildings Ltd | Construction | 2021 |
| Forefront Consultancy Limited | Professional & scientific | 2013 |
| Forefront IT Security Services Ltd | Information & communication | 2021 |
| Foreign Brothers Ltd | Hospitality | 2023 |
| Forest Carpentry & Construction Limited | Construction | 2022 |
| Forest Complete Carpentry Limited | Construction | 2017 |
| Forest Complete Properties Limited | Real estate | 2020 |
| Forest Drainage Products Limited | Manufacturing | 2004 |
| Forest Economic Partnership CIC | Other services | 2019 |
| Forest Gems Ltd | Manufacturing | 2015 |
| Forest of Dean & WYE Valley Tourism Ltd | Other services | 2012 |
| Forest of Dean Fabrication Limited | Manufacturing | 2020 |
| Forest Products Limited | Manufacturing | 1927 |
| Forest Rise Management Company Limited | Households | 2005 |
| Forest Safety Products Limited | Manufacturing | 2000 |
| Forest Shift Limited | Transport & logistics | 2025 |
| Forester Holdings Limited | Administrative & support | 1922 |
| Forever Farm Ltd | Agriculture & fishing | 2026 |
| Forever Funny Limited | Manufacturing | 2016 |
| Forever Interiors Ltd | Construction | 2023 |
| Forge Engineering Solutions Ltd | Manufacturing | 2021 |
| Fork and Spoon Ltd | Hospitality | 2019 |
| Forksy Limited | Hospitality | 2025 |