| Farr Physiotherapy Ltd | Other services | 2025 |
| Farrant Consulting Ltd | Information & communication | 2011 |
| Farriers Court Gloucester Limited | Households | 1992 |
| Farthinghall Developments Limited | Real estate | 2011 |
| Farthinghall Hanworth Limited | Extraterritorial | 2015 |
| Fascia Focus Limited | Wholesale & retail | 2003 |
| Fast Fit Garage Doors Limited | Construction | 2018 |
| Fast FIX (Gloucester) Limited | Wholesale & retail | 1992 |
| Fast Scrap Cars Limited | Wholesale & retail | 2021 |
| Fast Solutions Limited | Professional & scientific | 2002 |
| Fast Tools (2015) Limited | Wholesale & retail | 1966 |
| Fast Tools (South Wales) Ltd | Wholesale & retail | 2018 |
| Fasthosts Internet Limited | Information & communication | 1998 |
| Fastoncall Ltd | Other services | 2018 |
| Fastplant Ltd | Administrative & support | 2020 |
| FAT LIP Films Limited | Information & communication | 2018 |
| FAT Toni's Franchise Management Limited | Hospitality | 2020 |
| Fatal-Push Ltd | Wholesale & retail | 2025 |
| Father Foundry Ltd | Education | 2018 |
| Fatica Ltd | Real estate | 2017 |
| Favour A/C Foods Ltd | Wholesale & retail | 2020 |
| Favourite Beers Limited | Wholesale & retail | 2010 |
| Fayes Consulting Limited | Professional & scientific | 2010 |
| FB Facilitates Ltd | Administrative & support | 2025 |
| Fbus Logistic Limited | Transport & logistics | 2025 |
| FD9 Ltd | Professional & scientific | 2026 |
| Fdph. Limited | Health & social care | 2022 |
| Fears Property Services Limited | Construction | 2022 |
| Fedin's Ltd | Transport & logistics | 2023 |
| Feeding Tiny Minds CIC | Education | 2024 |
| Felce Foods Limited | Wholesale & retail | 2014 |
| Felice Holdings Ltd | Financial services | 2025 |
| Felicity & Co Hair Studio Ltd | Other services | 2025 |
| Fellowssurance Limited | Professional & scientific | 2024 |
| Feltham Tech Ltd | Information & communication | 2023 |
| Female Beauty Kingdom Ltd | Transport & logistics | 2021 |
| Fendwrap Global Ltd | Wholesale & retail | 2023 |
| Fenice IT Ltd | Administrative & support | 2016 |
| Fenn Collection UK Limited | Wholesale & retail | 2018 |
| Fentec Solutions Limited | Professional & scientific | 2020 |
| FEP PAY Limited | Information & communication | 2009 |
| Fern Lodgings Limited | Real estate | 2002 |
| Ferris Electrical Services Ltd | Construction | 2022 |
| Fertilityhr Technologies Limited | Information & communication | 2025 |
| Fewrooms.Com Limited | Administrative & support | 2017 |
| Feztree Limited | Arts & recreation | 1980 |
| FG Immigration Ltd | Professional & scientific | 2017 |
| FHC Socials Limited | Information & communication | 2025 |
| FHE Technology Ltd | Information & communication | 2007 |
| FHN Limited | Administrative & support | 2025 |
| FHP Software Ltd | Information & communication | 2025 |
| Fickle Love Ltd | Wholesale & retail | 2026 |
| Fidus Build Ltd | Construction | 2025 |
| Field Court Junior School | Education | 2011 |
| Fielding Global Limited | Professional & scientific | 2018 |
| Fifth Element Systems Limited | Information & communication | 2000 |
| FIG Electrical Ltd | Construction | 2012 |
| Fijiiuk Limited | Administrative & support | 2010 |
| Filders Ltd | Health & social care | 2023 |
| Filiz Ltd | Wholesale & retail | 2012 |
| Filter Engineering Limited | Manufacturing | 1992 |
| Fin-Q-Tel Ltd | Information & communication | 2023 |
| Finance Drive Gloucester Ltd | Professional & scientific | 2025 |
| Finance Efficiency Limited | Professional & scientific | 2020 |
| Financial Fitness Accounting Ltd | Professional & scientific | 2016 |
| Financial Fitness Group Ltd | Financial services | 2015 |
| Finch Inferno Ltd | Information & communication | 2025 |
| Finchcroft Court Management Company Limited | Households | 1996 |
| Finco Automotive Ltd | Professional & scientific | 2024 |
| Find Property Solutions Limited | Real estate | 2022 |
| Find Your Purpose Ltd | Professional & scientific | 2022 |
| Find Your Stride Coach Ltd | Arts & recreation | 2026 |
| Findout 2 Limited | Real estate | 2007 |
| Finer Finish Plastering Limited | Construction | 2019 |
| Finest Films Ltd | Information & communication | 2011 |
| Finesteps Ltd | Arts & recreation | 2014 |
| Finework Polishing Limited | Manufacturing | 2019 |
| Finishing Touches Property Maintenance Ltd | Construction | 2019 |
| Finkle Advisory Limited | Information & communication | 2025 |
| Finnegan SIX Ltd | Manufacturing | 2022 |
| Fins Global Limited | Real estate | 2023 |
| Fiona Leatt Ltd | Information & communication | 2016 |
| Fire and Ice Wellness Ltd | Wholesale & retail | 2024 |
| Fire Engineer Ltd | Professional & scientific | 2024 |
| Fire Phoenix Ltd | Manufacturing | 2026 |
| Firemad Ltd | Construction | 2024 |
| Firenze Gloucester Ltd | Hospitality | 2025 |
| First AID and Trauma Training Limited | Education | 2013 |
| First Call Grab Hire Limited | Extraterritorial | 2017 |
| First Call Pharmacy Ltd | Wholesale & retail | 2017 |
| First Call Plant Group Limited | Extraterritorial | 2017 |
| First Call Plant Hire Limited | Administrative & support | 2016 |
| First Call Plant Limited | Extraterritorial | 2017 |
| First Call Plant Rental Limited | Extraterritorial | 2017 |
| First Call Stone Supplies Limited | Extraterritorial | 2017 |
| First Choice Housing Limited | Hospitality | 2025 |
| First Class Furniture Group Limited | Wholesale & retail | 2022 |
| First Class Second Hand Ltd | Wholesale & retail | 2021 |
| First Thought Care Services Ltd | Health & social care | 2020 |
| First Thought Care Training Ltd | Education | 2024 |