| Enduro Sciences Limited | Manufacturing | 2020 |
| Energy Investment Projects Ltd | Wholesale & retail | 2010 |
| Energy Monkey Ltd | Wholesale & retail | 2019 |
| Energy Store (Solar) Ltd | Wholesale & retail | 2016 |
| Engineering Design & Staffing Solutions Limited | Professional & scientific | 2002 |
| Engineering Integration Consulting Ltd | Extraterritorial | 2021 |
| Engineering Integration Investments Ltd | Financial services | 2016 |
| Engineering Integration Ltd | Professional & scientific | 2014 |
| Enginehawk Limited | Wholesale & retail | 2020 |
| Engines Plus Limited | Wholesale & retail | 2009 |
| Enginia Limited | Professional & scientific | 2013 |
| Englewood Commercial Ltd | Professional & scientific | 2012 |
| Enhance Payroll Solutions Limited | Extraterritorial | 1989 |
| Enigma Smiles Ltd | Health & social care | 2023 |
| Enjosi Services Limited | Transport & logistics | 2017 |
| Enjoy Maths Limited | Education | 2024 |
| Enlyte Ltd | Information & communication | 2011 |
| Enodo AI Ltd | Manufacturing | 2026 |
| Enough to Feed Limited | Education | 2018 |
| Enrich Properties Ltd | Real estate | 2020 |
| Entansa Ltd | Information & communication | 2025 |
| Entrada Ltd | Professional & scientific | 2010 |
| Entrance Supplies Direct Ltd | Construction | 2021 |
| Entrepreneur Consulting Ltd | Financial services | 2024 |
| Entropic Ltd | Professional & scientific | 2025 |
| Envesca Ltd | Administrative & support | 2003 |
| Enviroguard (Holdings) Limited | Financial services | 2025 |
| Enviroguard (Northwest) Limited | Water & waste | 2006 |
| Environmental and Building Services Limited | Administrative & support | 2001 |
| EO Management Limited | Real estate | 2025 |
| EPC Dynamix Ltd | Professional & scientific | 2022 |
| Epic Adventures Travel Ltd | Administrative & support | 2025 |
| Epicus Data Limited | Information & communication | 2014 |
| Epicus Property Limited | Real estate | 2019 |
| Epochrebel Ltd | Professional & scientific | 2024 |
| EPP Holdings Ltd | Financial services | 2019 |
| EPR Surfacing Ltd | Construction | 2026 |
| Epthree Consultancy Limited | Administrative & support | 2010 |
| EQD Limited | Wholesale & retail | 1991 |
| Equal Electrical Services Limited | Construction | 2019 |
| Equila Consultancy Ltd | Professional & scientific | 2018 |
| Equilaw Limited | Professional & scientific | 2010 |
| Equipment Support Partners Limited | Professional & scientific | 2026 |
| Equisera Ltd | Manufacturing | 2022 |
| Equivet Therapies Ltd | Professional & scientific | 2017 |
| Erect a Scaffold Ltd | Construction | 2012 |
| Erhan Ogruc Bookkeeping & Financial Services Ltd | Financial services | 2018 |
| Eric Grabham Landscaping Ltd | Administrative & support | 2019 |
| Erimus Automobiles Limited | Wholesale & retail | 2015 |
| Erising C Limited | Information & communication | 2023 |
| Ermin Drive Management Company Limited | Households | 2020 |
| Ermin Park Limited | Construction | 2021 |
| Ermin Plant (Hire & Services) Limited | Administrative & support | 1965 |
| Eroga DIE Company Limited | Manufacturing | 1967 |
| Eroticats Ltd | Extraterritorial | 2011 |
| Errol Williams Limited | Wholesale & retail | 2015 |
| ESC Spinnaker Ltd | Professional & scientific | 2011 |
| Escape Kampers Limited | Wholesale & retail | 2025 |
| Escape Time (ST Ives) Limited | Arts & recreation | 2017 |
| Escend Ltd | Information & communication | 2009 |
| Esda Limited | Wholesale & retail | 2010 |
| Eshopnow.Co.Uk Limited | Wholesale & retail | 2021 |
| Esile Limited | Real estate | 2016 |
| Esmes Bar & Resturant Ltd | Hospitality | 2025 |
| Esprada Ltd | Wholesale & retail | 2024 |
| Espsp Ltd | Professional & scientific | 2020 |
| ESS Advisory Ltd | Professional & scientific | 2026 |
| Essential Care Solutions Ltd | Administrative & support | 2021 |
| Essential Washrooms Ltd | Manufacturing | 2025 |
| EST Terra Holdings Limited | Professional & scientific | 2016 |
| Esta Property Management Ltd | Real estate | 2025 |
| Estrella Wedding Shows Limited | Administrative & support | 2009 |
| ETA Nomad Auto Ltd | Administrative & support | 2026 |
| Eternal Book Publishers Ltd | Information & communication | 2025 |
| Eternity Support Limited | Administrative & support | 2023 |
| ETF 11 Limited | Professional & scientific | 2011 |
| Etiquette Hire Ltd | Hospitality | 2026 |
| Eudaimonia IT Services Ltd | Information & communication | 2025 |
| Eumart Ltd | Wholesale & retail | 2021 |
| Europa Tyres Limited | Professional & scientific | 1984 |
| European Alcohol Gloucester Ltd | Administrative & support | 2018 |
| European Christian Mission (International) | Other services | 1997 |
| European Union Shop Ltd | Wholesale & retail | 2025 |
| Euroseal Trade Sales Limited | Manufacturing | 2004 |
| Euroutes Ltd | Wholesale & retail | 2024 |
| EV Future Solutions Ltd | Energy | 2020 |
| Evangelism Explosion (Great Britain) Limited | Education | 1975 |
| Evans Electrical Services Limited | Construction | 2003 |
| Evans GBR Ltd | Construction | 2017 |
| Evans Mechanical Services Limited | Construction | 2017 |
| Evans Property Contractors Ltd | Construction | 2024 |
| Evansworth Holdings Limited | Financial services | 2025 |
| Evansworth Property Limited | Hospitality | 2025 |
| Evbay Ltd | Wholesale & retail | 2025 |
| Event Coffee Limited | Wholesale & retail | 2023 |
| Event Trader Limited | Wholesale & retail | 2022 |
| Event Water Ltd | Water & waste | 2017 |
| Eventageous PR Limited | Administrative & support | 2005 |
| Eventing GIN Limited | Wholesale & retail | 2019 |
| Ever Isabella Ltd | Manufacturing | 2025 |