| El-Perazim Limited | Wholesale & retail | 2025 |
| Elav Investments Ltd | Information & communication | 2024 |
| ELB Building Restorations Ltd | Construction | 2023 |
| ELB Electrical (Gloucester) Ltd | Construction | 2014 |
| ELB Ventures Ltd | Financial services | 2026 |
| Eldradesk Limited | Information & communication | 2025 |
| Electric Vehicle Training Ltd | Education | 2026 |
| Electric, GAS & Compliance Services Ltd | Construction | 2025 |
| Elegant Impressions Limited | Wholesale & retail | 2023 |
| Elemental Construction & Cabins Ltd | Construction | 2022 |
| Elevate FBE Ltd | Construction | 2021 |
| Elevate Talent Limited | Professional & scientific | 2016 |
| Eleven Builders Ltd | Construction | 2019 |
| Eleven Studio Ltd | Other services | 2024 |
| Elexar Ltd | Financial services | 2024 |
| Elichem Resins U.K. Limited | Construction | 2001 |
| Elite Electrical Installations (Glos) Ltd | Construction | 2026 |
| Elite Extrusion DIE Limited | Manufacturing | 1986 |
| Elite Facilities Management Ltd | Administrative & support | 2021 |
| Elite Feeds Limited | Wholesale & retail | 1983 |
| Elite Global Events Ltd | Administrative & support | 2025 |
| Elite Heritage Assets Ltd | Wholesale & retail | 2026 |
| Elite Home Care Solutions (UK) Ltd | Health & social care | 2012 |
| Elite Software Technologies Limited | Information & communication | 2017 |
| Elite Transfers Gloucestershire Ltd | Transport & logistics | 2023 |
| ELITE1BUILD Ltd | Construction | 2021 |
| Eliteclean BL Ltd | Administrative & support | 2025 |
| Eliteride Taxis Ltd | Transport & logistics | 2025 |
| Elizabeth Morris Film Limited | Information & communication | 2023 |
| Elkins Building Services Ltd | Construction | 2004 |
| Ella and Fleur Hot Yoga Limited | Arts & recreation | 2012 |
| Ellins Property Ltd | Real estate | 2026 |
| Elliot Entreprise Ltd | Wholesale & retail | 2025 |
| Elliott & Morgan Ltd | Administrative & support | 2024 |
| Elliott for Business Ltd | Administrative & support | 2026 |
| Ellis Accountants Ltd | Professional & scientific | 2013 |
| Ellis Electrical Contractors Ltd | Construction | 2014 |
| Ellis Group Holdings Ltd | Real estate | 2023 |
| Ellis Holiday Properties Limited | Real estate | 2024 |
| Ellis Property Limited | Real estate | 2017 |
| Ellis Property Solutions Ltd | Construction | 2016 |
| Ellisshop Ltd | Wholesale & retail | 2024 |
| Ellivron Investments Limited | Financial services | 1989 |
| Ellivron Optical Limited | Manufacturing | 2021 |
| Elmar Risk Management Limited | Professional & scientific | 2024 |
| Elmbridge Childcare Club Ltd | Other services | 2018 |
| Elmbridge Homes Limited | Real estate | 2004 |
| Elmbridge Pump Company Ltd | Manufacturing | 2013 |
| Elmbridge Supplies Company UK Ltd | Manufacturing | 2011 |
| Elmbury Properties Limited | Real estate | 2016 |
| Elmco Limited | Administrative & support | 2003 |
| Elmor Templar Limited | Real estate | 2005 |
| Elmore Court Limited | Extraterritorial | 2012 |
| Elmrep Office Solutions Limited | Administrative & support | 1998 |
| Elmscroft Community Centre CIC | Other services | 2021 |
| Elmstone Management Company Limited | Households | 2015 |
| Elolina Limited | Health & social care | 2024 |
| Eloquex Ltd | Information & communication | 2005 |
| Elote Ltd | Hospitality | 2023 |
| ELP Gloucestershire Ltd | Education | 2023 |
| Elpis Recruitment Ltd | Administrative & support | 2023 |
| Elroi Foods Ltd | Wholesale & retail | 2024 |
| Elseifwayes UK Ltd | Professional & scientific | 2024 |
| Elsha Property Rental Management Ltd | Real estate | 2020 |
| Emailogic Holdings Limited | Financial services | 2023 |
| Emailogic Ltd | Information & communication | 1998 |
| Embody & Express Ltd | Health & social care | 2025 |
| Emdc Contracting Ltd | Other services | 2021 |
| Emdr Equipment 2022 Ltd | Manufacturing | 2022 |
| Emenda Therapeutics Ltd | Professional & scientific | 2018 |
| Emerald 13 Limited | Wholesale & retail | 2024 |
| Emergingstars Ltd | Wholesale & retail | 2025 |
| Emery Builders Limited | Construction | 2025 |
| Emeson Care Services Limited | Administrative & support | 2025 |
| EMF Renewables Ltd | Construction | 2025 |
| EMG Civil Engineering Limited | Construction | 2019 |
| EMG Group Limited | Extraterritorial | 2015 |
| Emhl (Propco) Limited | Financial services | 2020 |
| Emil Z Trans Ltd | Transport & logistics | 2024 |
| Emita Polo (UK) Ltd | Wholesale & retail | 2023 |
| Emiwex Projects Limited | Information & communication | 2025 |
| Emma Priestley Sales & Marketing Ltd | Wholesale & retail | 2022 |
| Emma Warren Ltd | Wholesale & retail | 2018 |
| Emmalex Ltd | Information & communication | 2012 |
| Emmanuel U. Chukwuonye Ltd | Wholesale & retail | 2026 |
| Emmaus Gloucestershire | Wholesale & retail | 1998 |
| Emperia Structural Engineering Ltd | Professional & scientific | 2025 |
| Emperor Motors Ltd | Wholesale & retail | 2023 |
| Employee Stamp Tech Ltd | Information & communication | 2024 |
| Emporium Retailers Ltd | Wholesale & retail | 2024 |
| Empova Consulting Ltd. | Professional & scientific | 2017 |
| Empowered-Edu Foundation C.I.C. | Education | 2024 |
| EMR Trading Ltd | Hospitality | 2025 |
| EMS Equine Sport Therapy Ltd | Agriculture & fishing | 2024 |
| Enact IT Ltd | Information & communication | 2019 |
| Enchanting Childcare (Quedgeley) Ltd | Education | 2025 |
| Enchanting Childcare Ltd | Education | 2019 |
| Encompass Construction Services Limited | Construction | 2015 |
| END2END Management Limited | Professional & scientific | 2011 |
| Endure Performance Ltd | Other services | 2025 |