| "Luxura Homes Ltd | Real estate | 2021 |
| &Go Limited | Manufacturing | 2022 |
| (A) Property Sales Limited | Real estate | 1978 |
| 1 & 1A Lynmouth Road Management Company Limited | Households | 2011 |
| 1 - 2 - 1 Project Management Limited | Professional & scientific | 2004 |
| 1 ARM Ltd | Real estate | 1999 |
| 1 BAM Limited | Financial services | 2015 |
| 1 EFM Limited | Financial services | 2014 |
| 1 J Homes Limited | Real estate | 2025 |
| 1 Sunderland Terrace Limited | Real estate | 1996 |
| 100 Bath Road Management Company Limited | Real estate | 2016 |
| 101 Environmental Ltd | Information & communication | 2021 |
| 11 Kingsholm Road Management Limited | Households | 1983 |
| 11 North Quay Ltd | Real estate | 2021 |
| 11 Plus Leap Ltd | Education | 2018 |
| 112 London Road Limited | Extraterritorial | 2019 |
| 12 ST Catherine Street (Gloucester) Limited | Households | 2002 |
| 123 Car Commercial Limited | Wholesale & retail | 2008 |
| 123 Car Commercial Workshop Ltd | Wholesale & retail | 2022 |
| 12K Padel Ltd | Manufacturing | 2025 |
| 13 Brunswick Square (Gloucester) Limited | Professional & scientific | 1982 |
| 13 Heathville Road Management Company (1993) Limited | Households | 1993 |
| 1327 Records Ltd | Arts & recreation | 2025 |
| 13446309 Ltd | Administrative & support | 2021 |
| 14 Brunswick Square Management Company Limited | Households | 1985 |
| 14 Parkend Road Limited | Hospitality | 2007 |
| 14 Stanwick Road Limited | Households | 1997 |
| 140 Hempsted Lane (Gloucester) Management Limited | Real estate | 2014 |
| 155 Leighton Road Limited | Households | 1998 |
| 16 Newland Street Management Company Ltd | Households | 2018 |
| 16 Plus Care Support Solutions Ltd | Administrative & support | 2017 |
| 16 Sydenham Villas Road, Cheltenham Management Company Limited | Households | 1982 |
| 1875COMMS Limited | Information & communication | 2025 |
| 19 Ennismore Gardens Limited | Professional & scientific | 2001 |
| 1979 Limited | Other services | 2018 |
| 1ST Call Grab Hire Limited | Extraterritorial | 2017 |
| 1ST Call Hire Limited | Extraterritorial | 2016 |
| 1ST Call Plant Group Limited | Financial services | 2017 |
| 1ST Call Plant Hire Ltd | Administrative & support | 2020 |
| 1ST Call Plant Limited | Administrative & support | 2017 |
| 1ST Call Plant Rental Limited | Extraterritorial | 2017 |
| 1ST Call Stone Supplies Limited | Extraterritorial | 2017 |
| 1ST CDR Limited | Manufacturing | 2006 |
| 1ST Estate Service Ltd | Real estate | 2020 |
| 1STSECONDS Limited | Households | 2011 |
| 1UP Collective CIC | Education | 2016 |
| 2 Clarence Square (Cheltenham) Limited | Households | 1990 |
| 2 TEM Limited | Professional & scientific | 2015 |
| 2&6 Ltd | Real estate | 2013 |
| 20 Brunswick Square Limited | Households | 2016 |
| 20 Great Stanhope Street Limited | Administrative & support | 2002 |
| 20 London Road Management Company Limited | Households | 1982 |
| 20 NS Management Limited | Real estate | 2018 |
| 20 Parkside Close Management Company Limited | Households | 2013 |
| 20/20 - Vision Deliveries Ltd | Other services | 2019 |
| 2013 Solutions Ltd | Health & social care | 2013 |
| 21 Union Fitness Ltd | Arts & recreation | 2019 |
| 211 Productions Ltd | Administrative & support | 2026 |
| 23 - 28 Beamont Walk Residents RTM Company Limited | Households | 2021 |
| 24/7 South Gate Ltd | Wholesale & retail | 2026 |
| 2401 Visuals Ltd | Arts & recreation | 2023 |
| 247 Defender Limited | Financial services | 2020 |
| 247 UK Distribution Ltd | Transport & logistics | 2010 |
| 24HR Mobile Tyres Gloucestershire Ltd | Wholesale & retail | 2024 |
| 24KK Ltd | Wholesale & retail | 2026 |
| 25 Ingsholm Road Limited | Households | 2021 |
| 26 Evesham Road Management Company Limited | Administrative & support | 2004 |
| 26 Midland Road Management Limited | Households | 2005 |
| 270 Adventure Limited | Arts & recreation | 2016 |
| 2971 Ltd | Manufacturing | 2018 |
| 2B Aesthetic Ltd | Other services | 2024 |
| 2GC Electrical Contractors Ltd | Construction | 2019 |
| 2GR Trustees Limited | Real estate | 2013 |
| 2JAH Holdings Ltd | Wholesale & retail | 2022 |
| 2JIMS Limited | Construction | 2002 |
| 2MM Construction Ltd | Construction | 2022 |
| 2ND Chance (Gloucester) Ltd | Transport & logistics | 2021 |
| 2ND Chance First AID Limited | Education | 2026 |
| 2ND Chance Maintenance Ltd | Administrative & support | 2022 |
| 3 Ball Holdings Ltd | Financial services | 2021 |
| 3 Counties Enrg Ltd | Construction | 2025 |
| 3 Foot Tall Ltd | Wholesale & retail | 2023 |
| 3 Sisters Trading Ltd | Wholesale & retail | 2023 |
| 30ONE-GROUP Ltd | Construction | 2018 |
| 32 & 34 Shergar Close Management Company Limited | Real estate | 2009 |
| 32 Midland Road Gloucester Residents Management Company Limited | Real estate | 2003 |
| 32 Wellington Street, Gloucester Limited | Households | 2018 |
| 321 Three 2 One Limited | Transport & logistics | 2008 |
| 33 Lansdown Crescent RTM Company Limited | Households | 2012 |
| 35 Park Road (Management) Limited | Real estate | 1991 |
| 35/35A Aldridge Road Villas (Residents) Limited | Real estate | 1998 |
| 36 Brook Street Limited | Real estate | 2024 |
| 360 Automotive Gloucester Ltd | Wholesale & retail | 2014 |
| 360 Contract Services Ltd | Other services | 2009 |
| 365 Care Gloucester Limited | Administrative & support | 2026 |
| 365 Care Gloucestershire Ltd | Administrative & support | 2026 |
| 365 Care Needs Limited | Administrative & support | 2025 |
| 365 Care Worcester Limited | Administrative & support | 2026 |
| 37 Selkirk Street and 21 Glenfall Street Residents Company Limited | Households | 2000 |
| 39 Montpellier Terrace Management Company Limited | Households | 1993 |