| 3AMP Electrical Ltd. | Construction | 2025 |
| 3AP Homecare Limited | Health & social care | 2026 |
| 3D Interior Specialist Ltd | Construction | 2009 |
| 3J Transport Ltd | Transport & logistics | 2018 |
| 3KITES Property Ltd | Real estate | 2023 |
| 3V Natural Foods Limited | Professional & scientific | 2006 |
| 4 Pillars Construction Ltd | Construction | 2014 |
| 4 X Forge Limited | Extraterritorial | 2016 |
| 4/6 York Avenue Management Company Limited | Real estate | 2004 |
| 41 Moor Street Gloucester Management Company Limited | Real estate | 2012 |
| 41 Park Road Management Company Limited | Households | 2017 |
| 44 Black Diamonds Ltd | Financial services | 2019 |
| 44 Music Brands Ltd | Information & communication | 2026 |
| 44 Music Ltd | Information & communication | 2021 |
| 44/46 Worcester Street (Gloucester) Management Company Limited | Households | 1996 |
| 45 Pittville Lawn (Cheltenham) Limited | Households | 1984 |
| 47 Denmark Road Gloucester Residents Association Limited | Real estate | 1981 |
| 47 Park Road Management Company Limited | Households | 2020 |
| 4D Energy Ltd | Professional & scientific | 2010 |
| 4D Shopfitting Ltd | Manufacturing | 2016 |
| 4M Jewellers Limited | Professional & scientific | 2008 |
| 4M Jewellers Management Ltd | Financial services | 2018 |
| 4ORTY2 Ltd | Other services | 2007 |
| 4PW Ltd | Professional & scientific | 2018 |
| 4RANABROTHERS Ltd | Wholesale & retail | 2025 |
| 4RIIS Ltd | Administrative & support | 2025 |
| 5 Safety Limited | Administrative & support | 2014 |
| 5 ST George`S Square Management Company Limited | Households | 2007 |
| 51 Westgate Street Limited | Real estate | 2024 |
| 53 London Road Limited | Real estate | 2026 |
| 6 Brunswick Square Management Company Ltd | Households | 2025 |
| 6 Royal Parade (Cheltenham) Management Limited | Real estate | 1985 |
| 60-64 Eastgate Street Management Company Ltd | Households | 2016 |
| 7 Investors Limited | Financial services | 2021 |
| 7 Pittville Lawn Maintenance Limited | Households | 1996 |
| 71A Ltd | Professional & scientific | 2019 |
| 720 Group Limited | Professional & scientific | 2021 |
| 73 London Road Property Management | Households | 2016 |
| 781DGB Consultants Ltd | Professional & scientific | 2022 |
| 786 Furniture Warehouse Limited | Wholesale & retail | 2020 |
| 8 Construction Management Ltd | Construction | 2014 |
| 8 Lansdown Place Limited | Real estate | 2016 |
| 83 ST. Georges Road Management Company Limited | Households | 1996 |
| 9 Alexandra Road (Gloucester) Management Company Limited | Households | 2015 |
| 9 Dart DEN Limited | Wholesale & retail | 2025 |
| 9 Drummers Drumming Ltd | Education | 2025 |
| 95 Marylands Road Limited | Real estate | 2008 |
| 96RES Limited | Professional & scientific | 2020 |
| 97/99 Elmbridge Road Management Limited | Extraterritorial | 1999 |
| 99 Event Bars Ltd | Hospitality | 2025 |
| 99PINES Community Interest Company | Agriculture & fishing | 2025 |
| A & a Bird Holdings Ltd | Professional & scientific | 2020 |
| A & a Installations Ltd | Construction | 2024 |
| A & P Healthcare Ltd | Health & social care | 2022 |
| A & R Light Haulage Ltd | Transport & logistics | 2014 |
| A and T A2B Ltd | Transport & logistics | 2025 |
| A Bragg Transport Limited | Transport & logistics | 2016 |
| A C Avensis Limited | Real estate | 2002 |
| A C Design (UK) Limited | Professional & scientific | 2002 |
| A C Precision Limited | Manufacturing | 2007 |
| A D Burs Limited | Manufacturing | 1995 |
| A D Taylor Limited | Professional & scientific | 2002 |
| A E Heating (Glos) Ltd | Construction | 2024 |
| A G Burge Ltd | Professional & scientific | 2014 |
| A G Feakins Ltd | Construction | 2018 |
| A Girls Diary Limited | Information & communication | 2025 |
| A Human Business Limited | Professional & scientific | 2018 |
| A J & O G Associates Limited | Real estate | 2023 |
| A J Allen Carpentry Services Ltd. | Construction | 2014 |
| A J Edwards (Oakleigh) Ltd | Agriculture & fishing | 2014 |
| A L Markey Investment Co Ltd | Financial services | 2020 |
| A M Beaton Haulage Ltd | Transport & logistics | 2014 |
| A M Beaton Limited | Transport & logistics | 2019 |
| A M Direct Holdings Ltd | Financial services | 2025 |
| A M FEN and Co Ltd | Hospitality | 2017 |
| A M Hedi Ltd | Hospitality | 2024 |
| A M Property Solutions Ltd | Real estate | 2011 |
| A M Roofing & Cladding Ltd | Construction | 2025 |
| A Parekh Ltd | Health & social care | 2022 |
| A Partridge Ltd | Administrative & support | 2003 |
| A R DES Limited | Construction | 2014 |
| A R DEV Limited | Construction | 2012 |
| A R Graham Consultancy Limited | Hospitality | 2013 |
| A Star Taxis Ltd | Transport & logistics | 2018 |
| A Star Transport (Gloucester) Ltd | Transport & logistics | 2020 |
| A&A Electrical & Renewables Ltd | Construction | 2025 |
| A&A Mobile Solutions Ltd | Wholesale & retail | 2026 |
| A&B Property Care Limited | Administrative & support | 2004 |
| A&M Energy Fires Ltd | Manufacturing | 2020 |
| A&R Caruana Properties Limited | Real estate | 2025 |
| A&R Fast Food Ltd | Hospitality | 2017 |
| A&S Management Limited | Administrative & support | 2015 |
| A-Tech Heating Services Ltd | Construction | 2025 |
| A.B Construction (Glos) Limited | Construction | 2008 |
| A.C. Axiom Ltd. | Real estate | 2003 |
| A.D.B. Electrical Services Limited | Construction | 2005 |
| A.Frankley Limited | Administrative & support | 2022 |
| A.M. Dawson Holdings Limited | Real estate | 2023 |
| A.M.S. Fleet Management Limited | Wholesale & retail | 1999 |
| A.R.D. Developments Limited | Construction | 2000 |