| SLO Drinks (Asia Pacific) Limited | Manufacturing | 2026 |
| SM Electrical Solutions Ltd | Construction | 2015 |
| Smith's Bricks Limited | Real estate | 2025 |
| SMR Consultants Limited | Information & communication | 2024 |
| SMR Engineering Limited | Construction | 2009 |
| SMS Projects Limited | Real estate | 2025 |
| SN MCR Limited | Hospitality | 2022 |
| Solid Start 4D Ltd | Professional & scientific | 2012 |
| Solid Wood Products Ltd | Wholesale & retail | 2025 |
| Solidstart Ltd | Professional & scientific | 2020 |
| Soloc Limited | Manufacturing | 2019 |
| Solvemycoffee Ltd | Professional & scientific | 2024 |
| Soulful Therapy Limited | Other services | 2024 |
| South View Day Nursery Limited | Other services | 2001 |
| Southwest Fuel Recovery Ltd | Wholesale & retail | 2025 |
| Sowerbutts Greengrocers Ltd | Wholesale & retail | 2006 |
| SP Developments NW Ltd | Construction | 2025 |
| Sparked Homes Limited | Real estate | 2018 |
| Sparks Retail Advisors Ltd | Professional & scientific | 2023 |
| Spensley Consulting Ltd | Other services | 2013 |
| Spire Developments (NW) Limited | Real estate | 2020 |
| Spockles & Pops Holdings Ltd | Administrative & support | 2024 |
| Sponson Consulting Limited | Professional & scientific | 2018 |
| Spring Automotive Limited | Wholesale & retail | 2026 |
| Springheys Limited | Real estate | 2023 |
| Springwood Property Ltd | Real estate | 2020 |
| Ssmrv Limited | Wholesale & retail | 2018 |
| ST Marys RD (Glossop) Management Ltd | Real estate | 2024 |
| Stafford O'Neill Enterprises Ltd | Financial services | 2025 |
| Stag Compressors UK Limited | Manufacturing | 2024 |
| Steel FYI Ltd | Information & communication | 2022 |
| Stephen Cooke Limited | Wholesale & retail | 1978 |
| Stepping Stones Asset Management Limited | Real estate | 2016 |
| Stepping Stones Property Management Limited | Other services | 2011 |
| Steve Lonsdale Limited | Professional & scientific | 2024 |
| Straight UP Brickwork & Extensions Ltd | Construction | 2024 |
| Strategic Intelligence Limited | Information & communication | 2026 |
| Strawberry Shutters Limited | Wholesale & retail | 2017 |
| STS NW Limited | Construction | 2017 |
| Stylewise Developments Limited | Construction | 2024 |
| SUE Holt Limited | Financial services | 2017 |
| Sumner Property Developments Limited | Construction | 2023 |
| Sunningdale Commercial Properties Limited | Real estate | 2006 |
| Superbia Properties Ltd | Real estate | 2026 |
| Support Team Ltd | Administrative & support | 2017 |
| Surevest Capital Limited | Administrative & support | 2022 |
| Surevest Growth Limited | Professional & scientific | 2025 |
| Surevest Limited | Professional & scientific | 2024 |
| Surge Padel Limited | Arts & recreation | 2021 |
| Suzy's Sweet Memories Limited | Wholesale & retail | 2010 |
| Swallow Heritage Properties Limited | Real estate | 2025 |
| Swift Stitch Embroidery Ltd | Manufacturing | 2026 |
| Swiftcream Ltd | Wholesale & retail | 2021 |
| Sylvandale Property Limited | Real estate | 2005 |
| Synk Limited | Professional & scientific | 2008 |
| T & L High Peak Limited | Administrative & support | 2006 |
| T H Plastics Limited | Manufacturing | 2008 |
| T Hoyle Landscapes Limited | Administrative & support | 2026 |
| T&P Rental Limited | Real estate | 2019 |
| Tallow Bar and Grill Ltd | Hospitality | 2025 |
| Talnova Ltd | Education | 2025 |
| Tattum Ltd | Professional & scientific | 2020 |
| Tawny Barn Ltd | Manufacturing | 2022 |
| Taylor Made Designs (High Peak) Ltd | Other services | 2021 |
| Taylor Rose Developments Ltd | Construction | 2016 |
| TBS Engineering Southern Limited | Manufacturing | 2011 |
| Tea 2 Financial Limited | Financial services | 2024 |
| Team Vision Limited | Professional & scientific | 2003 |
| Tecexec Limited | Manufacturing | 1992 |
| Techo Interiors Limited | Construction | 2020 |
| TEL UK Ltd | Manufacturing | 2004 |
| Telcog Consultants Limited | Information & communication | 2024 |
| Telfer Capital Ltd | Real estate | 2025 |
| Temperature Electronics Limited | Manufacturing | 1969 |
| Teno Cutz Limited | Other services | 2025 |
| Terrain Ltd | Other services | 2023 |
| Testsonthenet Limited | Information & communication | 2014 |
| TEV NDT Ltd | Professional & scientific | 2022 |
| TH Fabrications (Glossop) Limited | Manufacturing | 2009 |
| TH Properties LLP | | 2014 |
| Thac-Cad Limited | Professional & scientific | 1996 |
| THE Alexander Group (NW) Ltd | Extraterritorial | 2017 |
| THE Architectural History Practice Limited | Professional & scientific | 1999 |
| THE Bakery (Hadfield) Limited | Manufacturing | 2009 |
| THE Bottle Store Ltd | Wholesale & retail | 2020 |
| THE Bulls Head Tintwistle Ltd | Hospitality | 2012 |
| THE Contrast Club Peak District Ltd | Other services | 2026 |
| THE Coppice (Glossop) Management Company Limited | Households | 2005 |
| THE Cove Properties NW Limited | Real estate | 2024 |
| THE Departure Board Ltd | Education | 2026 |
| THE Fighting Fit Shop Ltd | Other services | 2015 |
| THE Fountain Company Limited | Construction | 1998 |
| THE Friendship B & H Limited | Hospitality | 2017 |
| THE Gables NW Ltd | Hospitality | 2025 |
| THE Globe & Lift Limited | Hospitality | 2002 |
| THE Glossop Labour Club Limited | Hospitality | 2011 |
| THE Golf Hall Ltd | Wholesale & retail | 2026 |
| THE Good Gutter Guys Ltd | Construction | 2021 |
| THE Hairworx (Glossop) Limited | Professional & scientific | 2013 |
| THE Hayloft 2 Limited | Other services | 2003 |