| Hamilton Air Conditioning Services Ltd | Construction | 2022 |
| Hamilton Mcilroy Smith JNR Recycling Limited | Water & waste | 2015 |
| Hamilton-Green HR Services Ltd | Administrative & support | 2024 |
| Hamish Gordon Limited | Transport & logistics | 2012 |
| Handi at Glen Limited | Hospitality | 2025 |
| Hardie Developments Ltd | Construction | 2017 |
| Haus of Bronze Ltd | Other services | 2026 |
| Haustec UK Ltd. | Wholesale & retail | 2017 |
| HCS Aberdeen Limited | Mining & quarrying | 2018 |
| HCS Control Systems (Holdings) Limited | Professional & scientific | 2009 |
| HCS Control Systems Group Limited | Financial services | 2012 |
| HCS Control Systems Limited | Manufacturing | 1998 |
| Hedgehogs Nursery Limited | Wholesale & retail | 2005 |
| Henderson Heating Services Ltd | Construction | 2025 |
| Hhjr Consulting Limited | Professional & scientific | 2013 |
| Higgins Auto Shop Ltd | Wholesale & retail | 2024 |
| Highfield Hounds & Horses Limited | Agriculture & fishing | 2017 |
| Highland Halal Shop Ltd | Wholesale & retail | 2025 |
| Highlander Pest Management Ltd | Administrative & support | 2022 |
| Hochy Homes Limited | Real estate | 2017 |
| Hold Fast Promotions Limited | Wholesale & retail | 2025 |
| Holland and Pirrie Bespoke Interiors Ltd | Construction | 2025 |
| Holly Blue Consulting Limited | Information & communication | 2024 |
| Holo Trading Co Ltd | Wholesale & retail | 2026 |
| Homes 2 LET (Fife) Limited | Real estate | 2023 |
| HOP to Forage Ltd | Wholesale & retail | 2025 |
| Hotel IT Systems Consultancy Ltd | Information & communication | 2016 |
| House of GC Ltd | Other services | 2023 |
| House of Shaw Limited | Manufacturing | 2022 |
| HOW Human Limited | Professional & scientific | 2018 |
| Hshf Ltd | Real estate | 2020 |
| Hspcb Limited | Professional & scientific | 2025 |
| Hsva Ltd | Professional & scientific | 2026 |
| Hub 71 Ltd. | Arts & recreation | 2022 |
| Hunter Jack Ltd | Manufacturing | 2012 |
| Hunter NDT and Inspection Ltd | Professional & scientific | 2013 |
| Huspharma Ltd | Wholesale & retail | 2023 |
| Hutchison Investment Limited | Real estate | 2024 |
| Hutchison Trading Fife Limited | Real estate | 2024 |
| Hydrobotics Ltd | Professional & scientific | 2025 |
| HÁR Barber Limited | Other services | 2018 |
| I-Scaff Access Solutions Ltd | Construction | 2016 |
| I-Scaff Holdings Limited | Financial services | 2023 |
| I-Scaff Projects Ltd | Real estate | 2023 |
| IAN Gordon Financial Services (Scotland) Ltd | Financial services | 2019 |
| IAN Noble Training & Consultancy Ltd | Administrative & support | 2025 |
| IBRAHEEMASGHAR0077 Limited | Wholesale & retail | 2026 |
| Ibrahim X UN Limited | Professional & scientific | 2023 |
| ID Diesel Engine Specialists Limited | Professional & scientific | 2025 |
| ID Engineering Services Ltd | Manufacturing | 2025 |
| IHB Music Ltd. | Arts & recreation | 2011 |
| II Kolev Limited | Professional & scientific | 2024 |
| Iiem Electrical Limited | Construction | 2015 |
| Ikcloudsolutions Ltd | Professional & scientific | 2026 |
| Ilasco (Medical) Limited | Manufacturing | 2021 |
| Ilasco Limited | Wholesale & retail | 1980 |
| Immortalfabrication Ltd. | Manufacturing | 2014 |
| Impressive Memories Ltd | Manufacturing | 2022 |
| Inchdairnie Whisky Limited | Manufacturing | 2011 |
| Individual Cash Management Solutions Ltd. | Administrative & support | 2009 |
| Industrial Cladding and Roofing Solutions Limited | Construction | 2021 |
| Industrial Group International Ltd | Construction | 2021 |
| Industrial Roofing Scotland Ltd | Construction | 2014 |
| Industrial Safety Systems Ltd | Construction | 2024 |
| Innovative Tooling Solutions (I.T.S.) Limited | Manufacturing | 2005 |
| Insight Coaching & Consultancy Ltd | Professional & scientific | 2018 |
| Inspire Beauty Ltd | Other services | 2025 |
| Insurance Services (Scotland) Limited | Financial services | 1987 |
| International Magazine Centre Ltd | Information & communication | 2019 |
| Intrinsecus Holdings Limited | Real estate | 2020 |
| Inver Property (West Granton Road) Limited | Real estate | 2018 |
| Inver Property Group Limited | Real estate | 2016 |
| Iota Panel Systems Limited | Construction | 2004 |
| Iotl Ltd | Hospitality | 2018 |
| Iqbal 2016 Limited | Wholesale & retail | 2016 |
| Iqra Luqman Properties Ltd | Real estate | 2019 |
| Iraw Properties Limited | Real estate | 2025 |
| Irvine Meres Ltd | Professional & scientific | 2012 |
| Ises Properties Limited | Real estate | 2012 |
| Itek Systems Management Ltd | Information & communication | 2015 |
| Itken Limited | Administrative & support | 1982 |
| Iunctio Ltd | Information & communication | 2019 |
| Ivanhoe 1971 Limited | Extraterritorial | 2014 |
| J & K Financial Solutions Limited | Financial services | 2015 |
| J a Electrical Services Limited | Construction | 2022 |
| J Armstrong Building Contractors Ltd | Construction | 2024 |
| J Dingri Limited | Health & social care | 2024 |
| J E a Engineering (Fife) Limited | Manufacturing | 2014 |
| J Mackie Science Editing and Consulting Ltd | Professional & scientific | 2025 |
| J Mclure Inspection Limited | Construction | 2022 |
| J R F Chimney Specialists & Heating Distributors Limited | Construction | 1988 |
| J&W Murphy Limited | Transport & logistics | 2018 |
| J.B.S Inspection Limited | Manufacturing | 2016 |
| J10 Performance Football Ltd | Education | 2026 |
| JAA Solutions Ltd | Professional & scientific | 2024 |
| JAB DT Limited | Information & communication | 2024 |
| Jack Vettriano Limited | Wholesale & retail | 2002 |
| Jack Vettriano Publishing Limited | Information & communication | 2014 |
| Jackson & Co. (Fife) Limited | Professional & scientific | 2007 |
| Jafe Electrical Services Ltd | Manufacturing | 2025 |