| Flightplan Aviation Limited | Professional & scientific | 2016 |
| Float Fire Ice Ltd | Other services | 2025 |
| Flying Colours (Enterprises) Limited | Arts & recreation | 1994 |
| FMY Property Ltd | Real estate | 2016 |
| Focus Instrumentation Ltd | Professional & scientific | 2016 |
| Food Lounge GL Limited | Hospitality | 2022 |
| Forcell Surveys Ltd | Construction | 2016 |
| Foreversake Ltd | Manufacturing | 2021 |
| Forth and Thistle Limited | Wholesale & retail | 2025 |
| Forth Plasterers Ltd | Construction | 2015 |
| Forth Windows & Doors Ltd | Construction | 2012 |
| FOUNDRY4 Development Limited | Information & communication | 2026 |
| Four Oaks Investment Properties Limited | Real estate | 2025 |
| FOX Rental Properties Ltd | Real estate | 2024 |
| Fraser Gray Contracts Limited | Wholesale & retail | 1972 |
| Frederick Wilson (Properties) Limited | Construction | 2001 |
| Fudog Martial Arts Limited | Arts & recreation | 2017 |
| Fuel Drain Scotland Ltd | Wholesale & retail | 2024 |
| FUTURE2022 Ltd | Administrative & support | 2022 |
| FYI Driving School Ltd | Education | 2023 |
| G & G Skips and Refurbishment (Fife) Ltd | Other services | 2017 |
| G & I Groundworks Ltd | Construction | 2013 |
| G a Duncan Ltd | Construction | 2017 |
| G Tech Inspection Services Ltd | Construction | 2013 |
| G&J Lambert Properties Limited | Real estate | 2026 |
| G.K. Plumbing and Heating (Fife) Limited | Construction | 2025 |
| G2D Installations Limited | Construction | 2026 |
| G3 Flat Roofing Supplies and Installations Ltd | Construction | 2024 |
| Gadgetrepairstirling Ltd | Wholesale & retail | 2020 |
| Gair Properties Ltd | Real estate | 2025 |
| Gammies Country Clothing Limited | Wholesale & retail | 2009 |
| Gandaki Hospitality Limited | Hospitality | 2023 |
| Garage Design Ltd | Construction | 2023 |
| Garden Buildings Fife Ltd | Wholesale & retail | 2020 |
| Garrion Bridge Developments Ltd | Construction | 2021 |
| Gary Brown Services Ltd | Manufacturing | 2018 |
| GDS Slab Warehouse Limited | Wholesale & retail | 2022 |
| Gemmell Group Holdings Limited | Financial services | 2026 |
| Genzell Ltd | Agriculture & fishing | 2025 |
| George Dumitru Ltd | Transport & logistics | 2025 |
| George Fair Joiners & Contractors Ltd | Construction | 2015 |
| GGS Fife Ltd | Wholesale & retail | 2022 |
| GH Inspection Services Ltd | Professional & scientific | 2018 |
| Gheorghes Materials Quality Limited | Other services | 2026 |
| Gibson Rigging Limited | Administrative & support | 2018 |
| Gies a Hand Ltd | Administrative & support | 2023 |
| Gigaloch Limited | Information & communication | 2020 |
| Gillies Investments Limited | Construction | 2018 |
| Gillytree Studio Limited | Wholesale & retail | 2018 |
| GKC Pipeline Engineering Ltd | Manufacturing | 2024 |
| Glass Bullet Productions Limited | Information & communication | 2007 |
| Glen Foods Limited | Hospitality | 2020 |
| Glenbrae Trading Ltd | Construction | 2015 |
| Glendale Mouldings Limited | Manufacturing | 1981 |
| Glendale Plastics Limited | Manufacturing | 1979 |
| Glenfarg Developments Ltd | Construction | 2018 |
| Glenmuick Limited | Real estate | 2024 |
| Glenrothes Adam Ltd | Administrative & support | 2024 |
| Glenrothes Baptist Church Limited | Other services | 2021 |
| Glenrothes Dental House Ltd | Health & social care | 2025 |
| Glenrothes Football Club Community Interest Company | Arts & recreation | 2019 |
| Glenrothes Maintenance Services Ltd | Administrative & support | 2025 |
| Glenrothes Martial Arts Centre | Arts & recreation | 2006 |
| Glenrothes Motor Sport Club Limited | Arts & recreation | 1982 |
| Glenrothes Nails Ltd | Other services | 2023 |
| Glenrothes Phone Repair Limited | Manufacturing | 2020 |
| Glenspark Electrics Ltd | Construction | 2025 |
| Glenwood Take-Away Ltd | Hospitality | 2025 |
| Global Foods (Scotia) Ltd | Manufacturing | 2018 |
| Glovart Investments Limited | Real estate | 1976 |
| Go Metal Limited | Professional & scientific | 2012 |
| Goldcrest 48 Limited | Wholesale & retail | 2019 |
| Golo Golf Limited | Wholesale & retail | 2026 |
| GR8 Developments Limited | Administrative & support | 2001 |
| Gracemount Pavilions Ltd | Manufacturing | 2025 |
| Graham Bell Timber Harvesting Limited | Agriculture & fishing | 2009 |
| Grande Development Limited | Households | 2023 |
| Grange ST Andrews LLP | | 2021 |
| Great Scots Brewing Company Ltd | Manufacturing | 2024 |
| Greavo's BAT Repairs Ltd | Arts & recreation | 2024 |
| Green Shed Iona Limited | Real estate | 2026 |
| Greenbee Developments Limited | Administrative & support | 2021 |
| Greener Heating Solutions Ltd | Construction | 2022 |
| Griffiths Inspection and Training Services Ltd | Professional & scientific | 2017 |
| Grill 48 Ltd | Administrative & support | 2011 |
| Groundworx Outdoor Services Ltd | Administrative & support | 2026 |
| Grubb Inns Ltd | Other services | 2021 |
| Grupo Montaña Roja Limited | Financial services | 2023 |
| Gsangha Properties Limited | Real estate | 2026 |
| GSM Hospitality Limited | Professional & scientific | 2021 |
| GSR Properties Fife Ltd | Real estate | 2025 |
| GT Fincon Limited | Professional & scientific | 2025 |
| Gulkaari Ltd | Wholesale & retail | 2025 |
| GVM Ltd | Wholesale & retail | 2026 |
| GWM Associates Ltd | Professional & scientific | 2025 |
| H L M Cattle Services Ltd | Agriculture & fishing | 2018 |
| H3UNI | Education | 2015 |
| Haldane (UK) Limited | Manufacturing | 1975 |
| Haldane Limited | Manufacturing | 1998 |
| Halogen Communications Limited | Administrative & support | 2002 |