| "ALL Wrapped UP" Events Management Ltd | Administrative & support | 2007 |
| 0LIYA Ltd | Wholesale & retail | 2023 |
| 13NINE6 Stays Ltd | Real estate | 2024 |
| 1932 Taxi Ltd | Transport & logistics | 2014 |
| 2 BUX Limited | Real estate | 2020 |
| 2010 Distribution Ltd | Transport & logistics | 2010 |
| 2020 Recruitment Limited | Professional & scientific | 2025 |
| 24 Electrics Limited | Construction | 2021 |
| 323 Welding Ltd | Manufacturing | 2024 |
| 443 AI Ltd | Information & communication | 2018 |
| 56.4 North Property Development and Maintenance Limited | Real estate | 2022 |
| A G Brown Fabrications Limited | Manufacturing | 2012 |
| A N E Electrical Ltd | Construction | 2023 |
| A&M Properties Dundee Ltd | Hospitality | 2023 |
| A. Aslam Ventures Ltd | Real estate | 2025 |
| A. S. Sidhu Ltd | Health & social care | 2019 |
| A.Mckenzie Logistics Ltd | Transport & logistics | 2025 |
| A10 Architects Limited | Professional & scientific | 2010 |
| A2B Rentals Limited | Real estate | 2023 |
| AAA Guesthouse Ltd | Hospitality | 2016 |
| ABH (Fife) Limited | Hospitality | 2013 |
| Abodo Contractors Ltd | Construction | 2022 |
| ABS Interiors Ltd | Real estate | 2022 |
| Accurex Accounting Solutions Ltd | Professional & scientific | 2025 |
| Active House Alliance (UK Chapter) Ltd | Other services | 2022 |
| Active Treescape Ltd | Agriculture & fishing | 2008 |
| AD Multiply Ltd | Professional & scientific | 2014 |
| Adampackaging Ltd | Wholesale & retail | 2025 |
| Adamson Homes Limited | Real estate | 2019 |
| Adamson Sliding Doors Ltd | Construction | 2013 |
| Adcoles Electrical Ltd | Construction | 2025 |
| Adeel Enterprises Limited | Wholesale & retail | 2025 |
| Aden Contractors Ltd. | Construction | 2007 |
| Adita Cosmetics Ltd | Wholesale & retail | 2025 |
| Advanced Solutions Hub Ltd | Manufacturing | 2025 |
| Advancement of Recreational Therapy UK CIC | Health & social care | 2026 |
| AEH Inspection Ltd | Other services | 2021 |
| Afaans Limited | Wholesale & retail | 2026 |
| Afrobeat Entertainment Ltd | Administrative & support | 2022 |
| AG Finance Systems Ltd | Information & communication | 2025 |
| Agora Architecture and Design Limited | Professional & scientific | 2018 |
| AI Workspace Ltd | Information & communication | 2011 |
| Aiden Moffat Racing Ltd | Administrative & support | 2013 |
| AJ Property (Fife) Ltd | Real estate | 2025 |
| AJ Technical Ltd | Professional & scientific | 2026 |
| Ajmj Ltd | Hospitality | 2022 |
| Ajst Ltd | Hospitality | 2024 |
| AJT Properties Larkhall Ltd | Real estate | 2022 |
| AK B2L Ltd | Real estate | 2025 |
| AK Commercial Property Ltd | Real estate | 2025 |
| AK Retail Online Ltd | Wholesale & retail | 2025 |
| AL & ROO Events Ltd | Administrative & support | 2025 |
| Al's Motor Spares Ltd | Wholesale & retail | 2022 |
| Alan Cuts Barber Ltd | Other services | 2024 |
| Alan Knox Holdings Limited | Financial services | 2024 |
| Alan Sneddon Motors Ltd | Wholesale & retail | 2025 |
| Alex Mitchell Butchers Ltd | Wholesale & retail | 2012 |
| Alex Mitchell Limited | Wholesale & retail | 2013 |
| Alexa IT Limited | Wholesale & retail | 2026 |
| Alexander Smith JNR Recycling Ltd | Administrative & support | 2017 |
| ALG Inspection Limited | Professional & scientific | 2013 |
| Allson Sparkle Limited | Manufacturing | 1984 |
| Alltec Construction Ltd | Construction | 2003 |
| Alltec Energy Ltd | Construction | 2023 |
| Alltec Group Ltd | Construction | 2025 |
| Alltec Logistics Ltd | Transport & logistics | 2023 |
| ALS Civils Ltd | Construction | 2019 |
| Amber House 5588 Ltd | Hospitality | 2024 |
| Amber Tide Ltd | Administrative & support | 2025 |
| Ambition Centre for Training Limited | Education | 2005 |
| Ammo & I Ltd | Hospitality | 2018 |
| Anchor Stores Glasgow Ltd | Wholesale & retail | 2025 |
| Anderson Hall Leslie Ltd | Hospitality | 2025 |
| Anderson's Garage Fife Limited | Wholesale & retail | 2022 |
| Andor Cards Ltd | Information & communication | 2023 |
| Andor Holding Group Ltd | Financial services | 2018 |
| Andor Wills and Estates Ltd | Professional & scientific | 2024 |
| Andy Noble Electrical Ltd | Construction | 2019 |
| ANH Welding Services Limited | Manufacturing | 2013 |
| Antpa Properties Ltd | Real estate | 2025 |
| Apex Motors Specialists Limited | Wholesale & retail | 2023 |
| Apis Developments Limited | Construction | 1986 |
| Aquila Partners (Scotland) Ltd | Professional & scientific | 2024 |
| AR Easy Buy Ltd | Wholesale & retail | 2024 |
| Arbuckle Joinery Ltd | Construction | 2015 |
| ARC33 Investments Ltd | Real estate | 2026 |
| Arcstaw Scotland Limited | Construction | 2018 |
| Ardbeg Modular Limited | Construction | 2023 |
| Ardcanny Farm | | 1988 |
| Ardmel Automation Limited | Manufacturing | 1980 |
| Ardmel Group Limited | Professional & scientific | 1988 |
| Ardmel Precision Engineering Limited | Manufacturing | 2015 |
| Arnot Tower Holdings Limited | Financial services | 2025 |
| Arran Sidhu Portfolio Ltd | Real estate | 2020 |
| Artech Products Ltd | Construction | 2021 |
| Artistic Solutions (Scotland) Ltd | Arts & recreation | 2025 |
| Aryan Q Ltd | Wholesale & retail | 2023 |
| AS Cartify Limited | Wholesale & retail | 2025 |
| ASD Welding Services Limited | Manufacturing | 2011 |
| ASR Contractors Limited | Professional & scientific | 2021 |