| Asuk Removals Ltd | Water & waste | 2025 |
| Audio Emotion Limited | Wholesale & retail | 2008 |
| Ausmac Refrigeration Limited | Construction | 2026 |
| Automotive Automatics Ltd | Wholesale & retail | 2021 |
| Autumn Arcana Ltd | Wholesale & retail | 2025 |
| Avason Properties Limited | Real estate | 2016 |
| Aviation Academy Ltd | Education | 2024 |
| Aviation and Airfield Technical Services Ltd | Professional & scientific | 2026 |
| Ayaan Luqman Properties Ltd | Real estate | 2022 |
| B C B S R Developments Limited | Real estate | 2013 |
| B G Taylor Groundworks Limited | Construction | 2008 |
| B&B Blyth Property Limited | Real estate | 2025 |
| B&B Supplies Ltd | Wholesale & retail | 2025 |
| B&E Travel Limited | Administrative & support | 2012 |
| B2E Electrical & Renewables Ltd | Construction | 2025 |
| BAC Training Limited | Education | 2026 |
| Bags Boutique Ltd | Wholesale & retail | 2025 |
| Bakolina Limited | Health & social care | 2024 |
| Balanced and Thriving Ltd | Public administration | 2026 |
| Balanced Beauty Studio Ltd | Other services | 2026 |
| Balbirnie Fitness Centre Ltd. | Education | 2002 |
| Balbirnie House Hotel Limited | Hospitality | 1988 |
| Balgonie Embroidery and Prints Limited Limited | Manufacturing | 2024 |
| Balgonie Property Services Ltd | Real estate | 2013 |
| Balgowan Fine Foods Ltd | Manufacturing | 2024 |
| Balgowan Hospitality Solutions Ltd | Hospitality | 2025 |
| Bar 24 (Livingston) Ltd | Hospitality | 2017 |
| Barbers Glenrothes Ltd | Other services | 2025 |
| Bare Baked Ltd | Manufacturing | 2022 |
| Bare Brae Ltd | Wholesale & retail | 2021 |
| Bareddy Ltd | Construction | 2026 |
| Bargain Gateway Ltd | Wholesale & retail | 2018 |
| Barrie Bookkeeping & Payroll Solutions Limited | Financial services | 2011 |
| Bartolo Bathrooms & Interiors Ltd | Construction | 2025 |
| Basildene Developments Ltd | Construction | 2018 |
| Bastion Renovate & Build Limited | Construction | 2025 |
| Bdar 2019 Limited | Other services | 2019 |
| BE Findable Ltd | Professional & scientific | 2026 |
| BE Kind Live C.I.C. | Arts & recreation | 2020 |
| BE the Bride Boutique Ltd | Extraterritorial | 2021 |
| Beactica Therapeutics UK Limited | Professional & scientific | 2022 |
| Bearing Station Ltd | Wholesale & retail | 2009 |
| Beauty Boutique Fife Ltd | Other services | 2016 |
| Beauty Naturelle (Fife) Ltd | Other services | 2015 |
| Beauty@Rare Ltd | Other services | 2019 |
| Beckett Property Investment Ltd | Real estate | 2020 |
| Bell & Buist Joiners Limited | Construction | 2002 |
| Bellevue Medical Devices Ltd | Manufacturing | 2024 |
| Bernhew Consultancy Limited | Professional & scientific | 2007 |
| Berts Logistics Ltd | Transport & logistics | 2025 |
| Besec Systems Ltd. | Administrative & support | 2021 |
| Bespoke Developments Fife Ltd | Construction | 2025 |
| BG Services (Scotland) Limited | Construction | 2013 |
| Big Bridge Marketing Ltd | Wholesale & retail | 2011 |
| Bikers Boutique Limited | Wholesale & retail | 2010 |
| Bikeworks Fife Ltd | Manufacturing | 2008 |
| BIL & Friends Ltd | Wholesale & retail | 2024 |
| Bisset Contracting Limited | Construction | 2011 |
| Blachere Illumination UK Limited | Manufacturing | 1994 |
| Black Ocean Group Ltd | Information & communication | 2022 |
| Black Road Developments Ltd | Construction | 2018 |
| Blackwater Manufacturing Limited | Manufacturing | 1987 |
| Blackwater Stewart Limited | Professional & scientific | 2013 |
| Blackwood Offshore Services Ltd | Professional & scientific | 2017 |
| Blair Smart Limited | Construction | 2016 |
| Blairs Quality Foods Ltd | Wholesale & retail | 2026 |
| Bloomdawg Ltd | Wholesale & retail | 2025 |
| Bluebell Mobility Ltd | Wholesale & retail | 2026 |
| Blueprint Recruitment Limited | Administrative & support | 2007 |
| BMD Group Ltd | Wholesale & retail | 2026 |
| Boardwalk Bars & Bistros Limited | Hospitality | 2021 |
| Bomad Holdings Limited | Construction | 2016 |
| Boneyard Studios Ltd | Information & communication | 2025 |
| Bonnie Estates Limited | Construction | 2025 |
| Boreland Farm Limited | Hospitality | 2014 |
| Brianforbes Limited | Construction | 2025 |
| Bright Flamingo Ltd | Professional & scientific | 2021 |
| Bright Inspection Limited | Construction | 2011 |
| Bright Light Traffic Management Ltd | Other services | 2023 |
| Brinnor Limited | Wholesale & retail | 2009 |
| Broadwood Cars Limited | Wholesale & retail | 2015 |
| Broomfield Farm Cottage Limited | Real estate | 2026 |
| Brotherzz Properties Ltd | Real estate | 2023 |
| Brubare Ltd | Wholesale & retail | 2023 |
| Buaic ARD Ltd | Financial services | 2021 |
| Burmah (Holdings) Limited | Financial services | 2016 |
| Burmah Properties Limited | Real estate | 2016 |
| Burmah Self Drive & Lifting Services Ltd. | Transport & logistics | 2006 |
| Burmah Vehicle Sales Ltd | Wholesale & retail | 2016 |
| Burnett Group International Ltd | Construction | 2025 |
| Burns Installations Limited | Construction | 2015 |
| Business Gateway Fife | Information & communication | 2001 |
| BWM Systems Limited | Professional & scientific | 2016 |
| C & C Road Safety Consultants Limited | Construction | 2024 |
| C & F Securicom Limited | Administrative & support | 2012 |
| C Nelson Engineering Ltd | Wholesale & retail | 2023 |
| C.M.G.A. Limited | Professional & scientific | 2007 |
| Cable Road Containers Ltd | Transport & logistics | 2013 |
| Cabled IT Limited | Professional & scientific | 2016 |
| Cadham Car Wash Ltd | Administrative & support | 2026 |