| DNA Logistic South East Ltd | Transport & logistics | 2015 |
| DNN Property Limited | Real estate | 2018 |
| Dnymatch Limited | Information & communication | 2020 |
| Dobria Builders Ltd | Construction | 2023 |
| Doctorabc Ltd | Other services | 2017 |
| Documents UN Limited | Administrative & support | 2019 |
| Dodd's Brewery Co. Limited | Manufacturing | 1998 |
| Doddington Car Sales Limited | Wholesale & retail | 2023 |
| Dodge Easy MFG Co., Ltd | Wholesale & retail | 2024 |
| DOK Electrical Ltd | Construction | 2022 |
| Dolphin Mews Limited | Households | 2014 |
| Dolphin PUB Ltd | Hospitality | 2019 |
| Dolphins J T Cleaning Services Limited | Other services | 2019 |
| DOM Dominik Ltd | Transport & logistics | 2017 |
| Domco Ltd | Administrative & support | 2015 |
| Domoli Limited | Construction | 2024 |
| Dongsheng Commodity Co., Ltd | Manufacturing | 2022 |
| Doroo Ltd | Construction | 2018 |
| Dorset Acquisitions Ltd | Hospitality | 2016 |
| Dorset Catch Limited | Wholesale & retail | 2021 |
| Dorset Energy Solutions Ltd | Construction | 2010 |
| Dorset Motorcycles Ltd | Wholesale & retail | 2022 |
| Dorset Quality Services (DQS) Ltd | Professional & scientific | 2022 |
| Dorset RAW Pet Foods Ltd | Wholesale & retail | 2024 |
| Dotcom Analytics Ltd | Information & communication | 2024 |
| Double M Stud Limited | Agriculture & fishing | 2017 |
| Double M Studios Ltd | Information & communication | 2018 |
| Dovortex Ltd | Wholesale & retail | 2021 |
| Dowdstage Property & Development Limited | Real estate | 2020 |
| DP Flooring & Constructios Ltd | Construction | 2026 |
| DP Media Ltd | Arts & recreation | 2024 |
| Dpharmn Limited | Wholesale & retail | 2018 |
| DPR Consulting Services Limited | Other services | 2006 |
| DR Associates (UK) Limited | Construction | 2009 |
| DR Veneta Limited | Wholesale & retail | 2023 |
| Dr&R Construction Ltd | Construction | 2022 |
| Drainage Smart Ltd | Other services | 2019 |
| Dramatix Production Engineering Ltd | Manufacturing | 2022 |
| Dray Enterprise Limited | Administrative & support | 2020 |
| Dream Builder Partnership Ltd | Health & social care | 2024 |
| Dream Deals Holidays Ltd | Administrative & support | 2015 |
| Dream Team Production House Ltd | Professional & scientific | 2024 |
| Dreammy Faces Limited | Other services | 2025 |
| Dress Smart Initiative | Other services | 2025 |
| Dressing Room 5 Limited | Administrative & support | 2011 |
| Drift Coffee Company Ltd | Wholesale & retail | 2025 |
| Drivez Ltd | Transport & logistics | 2022 |
| Driving Plan Limited | Transport & logistics | 2015 |
| DRK Express Ltd | Financial services | 2025 |
| DRW Developments Ltd | Construction | 2018 |
| DS (30) HC Ltd | Arts & recreation | 2010 |
| Dstyles Ventures Ltd | Information & communication | 2016 |
| DSW2017 Ltd | Manufacturing | 2017 |
| DT Nova Ltd | Construction | 2026 |
| DT One Construction Ltd | Construction | 2017 |
| DTE Carpentry Ltd | Manufacturing | 2023 |
| DTK Solutions Limited | Professional & scientific | 2019 |
| DU International Limited | Administrative & support | 2003 |
| Dubai Shawarma Gillingham Ltd | Hospitality | 2025 |
| Dubai Shawarma Kent Ltd | Hospitality | 2026 |
| Dued App Ltd | Information & communication | 2023 |
| Duffy Controls and Designs Ltd | Construction | 2025 |
| Dunamis Beauty Limited | Other services | 2025 |
| Duncliffe Associates Ltd | Professional & scientific | 2013 |
| Duncliffe View Property Management Limited | Households | 1996 |
| Dunworth 100 Ltd | Administrative & support | 2012 |
| Duotech Electrical Services Ltd | Construction | 2026 |
| Dutra Motorcycles Ltd | Wholesale & retail | 2025 |
| DVM Catering Ltd | Hospitality | 2023 |
| DW Safeguarding Consultants Ltd | Professional & scientific | 2025 |
| DWC Home Improvements Ltd | Administrative & support | 2017 |
| Dwhr Solutions Ltd | Administrative & support | 2025 |
| DXB Management Services Ltd | Administrative & support | 2022 |
| DXJ Solutions Ltd | Information & communication | 2025 |
| DYE Bookkeeping Ltd | Professional & scientific | 2019 |
| Dynamic Plumbing Services Limited | Construction | 2021 |
| Dynanova Company Limited | Wholesale & retail | 2022 |
| E & E Foods Limited | Hospitality | 2014 |
| E G Properties ( Kent ) Ltd | Construction | 2021 |
| E K L Builders Limited | Construction | 2012 |
| E Stephens Consulting Limited | Real estate | 2016 |
| E&A Mixer Trucks Limited | Transport & logistics | 2025 |
| E&I Architects Ltd | Professional & scientific | 2023 |
| E&I Properties Ltd | Real estate | 2022 |
| E&I Residential Ltd | Real estate | 2025 |
| E.G.T Properties Ltd | Real estate | 2024 |
| E.Y.A.T Ltd | Hospitality | 2024 |
| Eanadpet Limited | Health & social care | 2022 |
| EAQ (Education and Qualifications) Ltd | Education | 2010 |
| Earnesta Trades & Services Ltd. | Education | 2015 |
| Earthwise Tree Care Kent Ltd | Agriculture & fishing | 2024 |
| Easom Plumbing and Heating Ltd | Construction | 2023 |
| Easonrise International Trade Limited | Extraterritorial | 2016 |
| East Mendip Developments Ltd | Construction | 2023 |
| East Surgery Ltd | Health & social care | 2025 |
| Easttai Industrial Limited | Extraterritorial | 2016 |
| Easypuck Ltd | Wholesale & retail | 2024 |
| Eazzy Healthcare Services Ltd | Health & social care | 2026 |
| Ebbsfleet Marine Limited | Other services | 1999 |
| Ebbsfleet Specialist Cars Limited | Wholesale & retail | 2014 |