| Gordon Brooke Farming Limited | Agriculture & fishing | 2015 |
| Gordon Community Woodland Trust | Other services | 2002 |
| Graham Mortgage Solutions Limited | Financial services | 2026 |
| Grange DXB Limited | Real estate | 2017 |
| Green Properties SL Ltd | Real estate | 2026 |
| Greenpower Electrical and Renewables Ltd | Construction | 2021 |
| Groundgear Ltd | Manufacturing | 2025 |
| Grow Forestry Ltd | Agriculture & fishing | 2020 |
| GUN Knowe Investments Ltd | Real estate | 2024 |
| Gwjm Golf Ltd | Arts & recreation | 2018 |
| GXE Telecom Ltd | Information & communication | 2018 |
| H + D Construction (Scotland) Ltd. | Construction | 2004 |
| H R Motors Limited | Manufacturing | 2015 |
| H. S. Riddell Timber Merchants Limited | Manufacturing | 2009 |
| Haircubez Limited | Other services | 2023 |
| Hall Opticians Limited | Wholesale & retail | 2015 |
| Hamilton Properties Ltd | Real estate | 2026 |
| Hank Marvin's Rolls Limited | Wholesale & retail | 2015 |
| Harkin Builders Limited | Construction | 2023 |
| Hawick & Border Car Club Limited | Arts & recreation | 1988 |
| Heera Food Galasiels Ltd | Hospitality | 2024 |
| Height Hub Limited | Professional & scientific | 2025 |
| Hendzel Craft Carpentry & Joinery Ltd | Administrative & support | 2017 |
| Hendzel Craft Partnership Ltd | Construction | 2022 |
| Herkes Funerals Limited | Other services | 2018 |
| Hijokoanu Global Ltd | Wholesale & retail | 2025 |
| Hillslap and Greenhill Limited | Agriculture & fishing | 1959 |
| Hilltop Maintenance Limited | Agriculture & fishing | 2014 |
| Hiyan Studio Ltd | Wholesale & retail | 2024 |
| HMD Contractors Limited | Construction | 2013 |
| Hogg & Mundell | | 1976 |
| Holequest Limited | Construction | 1974 |
| Home Energy Advancements Ltd | Construction | 2025 |
| Homephysio by Nicola Limited | Health & social care | 2021 |
| Honey BE Bright Ltd | Manufacturing | 2018 |
| Hour JAR Ltd | Administrative & support | 2018 |
| Howard's Paving & Surfacing Ltd | Administrative & support | 2023 |
| HRM Borders Limited | Real estate | 2025 |
| HSA Healthcare Limited | Professional & scientific | 2011 |
| HSW Assets Limited | Real estate | 2022 |
| Hunter Properties (Scotland) Limited | Real estate | 2018 |
| Hutchisons Carpets Limited | Wholesale & retail | 2013 |
| Hvac Ventilation Ltd | Construction | 2025 |
| Iain Marshall Limited | Manufacturing | 2007 |
| IAN Gajczak Farrier Limited | Professional & scientific | 2015 |
| IAN Mckelvie Bvms Mrcvs Ltd | Administrative & support | 2017 |
| Ice Equestrian Ltd | Agriculture & fishing | 2020 |
| Industry Skills Training Ltd | Education | 2023 |
| Infinite Power Solutions Ltd | Administrative & support | 2023 |
| Inis Online Ltd | Wholesale & retail | 2021 |
| INK Fever Ltd | Arts & recreation | 2023 |
| Interactx Ltd | Professional & scientific | 2023 |
| J & F,S Licensed Grocers Limited | Wholesale & retail | 1987 |
| J & R Dewar Ltd | Professional & scientific | 2025 |
| J D Smith Ltd | Professional & scientific | 2025 |
| J Stewart Plant & Commercial Ltd | Wholesale & retail | 2025 |
| J. Clyne Engineering Ltd | Manufacturing | 2025 |
| Jake's Courier Services Limited | Administrative & support | 2021 |
| James R Graham (Roofing) Ltd | Construction | 2009 |
| Jardine Electrification Limited | Transport & logistics | 2018 |
| JB Rentals Limited | Administrative & support | 2017 |
| JCD Training Limited | Professional & scientific | 2018 |
| JDC Property Management Ltd | Households | 2021 |
| Jeffrey Carpets and Flooring Limited | Wholesale & retail | 2019 |
| JF Bookkeeping Ltd | Professional & scientific | 2020 |
| JGM Blacksmiths Edinburgh Ltd | Manufacturing | 2025 |
| JIM Clark Memorial Motor Club Limited | Arts & recreation | 2006 |
| Jjrb Building Contractors Limited | Manufacturing | 2018 |
| Jjrb Properties Ltd | Real estate | 2024 |
| JL Consultants (Borders) Limited | Administrative & support | 2020 |
| John Graham Roofing (Selkirk) Limited | Construction | 2013 |
| Jordan Ferguson Engineering Limited | Construction | 2020 |
| JR Catering Limited | Hospitality | 2021 |
| Jscraft Ltd | Manufacturing | 2024 |
| Jubilee Travel Ltd | Transport & logistics | 2023 |
| Just Cycle Ltd | Education | 2015 |
| K&S Walker Approved Electricians Ltd | Construction | 2026 |
| Kailzie Equestrian Limited | Administrative & support | 2018 |
| Kazoffshore Ltd | Administrative & support | 2026 |
| Keith Braithwaite Limited | Construction | 2012 |
| Keith Mclean Carpets Limited | Wholesale & retail | 2005 |
| Kelly's Brow Studio Ltd | Other services | 2024 |
| Kemp Blair & Co Limited | Professional & scientific | 1991 |
| Kemp Blair Holdings Limited | Professional & scientific | 1897 |
| Kenkleen Limited | Administrative & support | 1993 |
| Kfdeane Limited | Health & social care | 2021 |
| Khadam Ltd | Hospitality | 2026 |
| Kidglover Limited | Hospitality | 2020 |
| Kinkell Finance Ltd | Financial services | 2013 |
| KJK Custom Fabrication Ltd | Manufacturing | 2025 |
| KRS Electrical Solutions Ltd | Construction | 2025 |
| Lammermoor Consulting Limited | Administrative & support | 2019 |
| Langlee Chinese Ltd | Hospitality | 2026 |
| Langlee Chipie Ltd | Hospitality | 2022 |
| Larch Property Developments Ltd | Real estate | 2017 |
| Lauderdale Preservation Group Ltd | Professional & scientific | 2025 |
| Lazy Evaluation Limited | Information & communication | 2012 |
| LBD Creative Ltd | Administrative & support | 2013 |
| Leaver Wellbeing Limited | Health & social care | 2017 |
| LEE Garden TD Ltd | Hospitality | 2023 |