| CWP Leith Limited | Construction | 2020 |
| D4CPROPERTYDEVELOPMENTS Ltd | Real estate | 2026 |
| D9 Scaffolding Ltd | Construction | 2018 |
| Dalgolly Ltd | Extraterritorial | 2012 |
| Dalvista Ltd | Hospitality | 2019 |
| Danny's A7 Car Wash Limited | Administrative & support | 2016 |
| Darzi Roots PVT Ltd | Wholesale & retail | 2025 |
| David Runciman (Poultry) Limited | Agriculture & fishing | 2019 |
| Davidson Baxter Partnership Limited | Professional & scientific | 1999 |
| Decades Gallery Ltd | Health & social care | 2024 |
| Dere Street Property Limited | Construction | 2014 |
| Deviant Solutions Ltd | Information & communication | 2018 |
| Dewar & Partners Scotland Limited | Financial services | 2011 |
| DFH Developments Ltd | Construction | 2024 |
| DHU Brew Limited | Wholesale & retail | 2015 |
| Diamond Cycle Centre Limited | Wholesale & retail | 2014 |
| Dilk Associates Limited | Professional & scientific | 2019 |
| Doogan Services Limited | Construction | 2011 |
| Down to Earth Foods Limited | Wholesale & retail | 2012 |
| Drew Graham Plant Services Limited | Construction | 2015 |
| Drummonds Mill Limited | Transport & logistics | 2020 |
| Dtcg Cleaning Limited | Administrative & support | 2023 |
| Duncan Mackinnon Music and Arts Trust Limited | Arts & recreation | 2011 |
| Durcol Property & Investments Limited | Construction | 2006 |
| DVE Flowers Limited | Wholesale & retail | 2019 |
| DW Mortgages Limited | Financial services | 2023 |
| E B Interiors Limited | Construction | 2023 |
| EAI Estates Ltd | Real estate | 2026 |
| Easy Asset Finance Ltd | Financial services | 2022 |
| Easyfix Group (Borders) Ltd | Construction | 2026 |
| Ebrhfitness Ltd | Other services | 2025 |
| ED Robertson Property Limited | Real estate | 2016 |
| Eddies Gardening Ltd | Administrative & support | 2025 |
| Edinburgh Diesel Ltd | Wholesale & retail | 2018 |
| Edinburgh Grab Hire Ltd | Professional & scientific | 2025 |
| Eildon Health and Fitness Ltd | Arts & recreation | 2024 |
| Eildon Projects Ltd | Construction | 2026 |
| Eildon Refractories Limited | Wholesale & retail | 1995 |
| ELC Electrical Ltd | Construction | 2025 |
| Element Vans Ltd | Manufacturing | 2024 |
| Elevatech Systems Limited | Information & communication | 2026 |
| Elliot Hook Limited | Manufacturing | 2006 |
| Elliot Page IT Consultancy Limited | Information & communication | 2022 |
| Elliotts Edinburgh Ltd | Hospitality | 2018 |
| Energy Systems Scotland Ltd | Construction | 2023 |
| Enteitcg Ltd | Wholesale & retail | 2025 |
| Environmental Surface Technology Limited | Manufacturing | 2022 |
| Enviroshift Limited | Water & waste | 2026 |
| Eventit Exhibitions Limited | Professional & scientific | 2015 |
| Eventsbase Limited | Professional & scientific | 2015 |
| Evercart Deals Ltd | Wholesale & retail | 2025 |
| Evinsiv Ltd | Information & communication | 2025 |
| Fadfinder Ltd | Wholesale & retail | 2024 |
| Fastmove Property Ltd | Real estate | 2025 |
| FBW Retail Group Limited | Wholesale & retail | 2024 |
| Ferguson Planning Ltd | Professional & scientific | 2018 |
| Fillmybus.Com Ltd | Wholesale & retail | 2017 |
| Finlaysons Contracts Limited | Construction | 2016 |
| Finrhu Consultancy Ltd | Professional & scientific | 2024 |
| Five Star Taxis (Scotland) Limited | Transport & logistics | 2009 |
| Fjordhus Construction Limited | Construction | 2006 |
| Fjordhus Limited | Construction | 1999 |
| Flexible Respite Ltd | Health & social care | 2015 |
| Flockoffitness Ltd | Other services | 2025 |
| Flow and Glow Pilates Ltd | Health & social care | 2025 |
| Flower Expressions Limited | Wholesale & retail | 2011 |
| Floyd and Benji Ltd | Wholesale & retail | 2024 |
| FLP Consultancy Limited | Administrative & support | 2004 |
| Forest Direct Limited | Agriculture & fishing | 2003 |
| Forth Computer Services Ltd | Information & communication | 2013 |
| Foster Care Connect Ltd. | Health & social care | 2004 |
| Fragrance Republic Limited | Wholesale & retail | 2023 |
| Freelance Solutions Limited | Transport & logistics | 2014 |
| Funky Frog Tours Limited | Administrative & support | 2019 |
| G & D Barrett Ltd | Hospitality | 2018 |
| G & G'S Takeaway Limited | Administrative & support | 2017 |
| G Aitchison Plumbing & Heating Ltd | Construction | 2021 |
| Gala 1960 Limited | Extraterritorial | 2013 |
| Gala Fairydean Rovers Football Club Limited | Hospitality | 2018 |
| Gala Takeaway Limited | Hospitality | 2019 |
| Galapate Limited | Hospitality | 2005 |
| Galashiels Doggy Daycare Ltd | Other services | 2020 |
| Galashiels Emporium Ltd | Wholesale & retail | 2026 |
| Galashiels Glass & Glazing Limited | Construction | 2020 |
| Galashiels Newsagent Limited | Wholesale & retail | 2025 |
| Galashiels Restaurants Limited | Hospitality | 2018 |
| Galashiels Storage Ltd | Administrative & support | 2017 |
| Galashiels Taxation Services Limited | Professional & scientific | 2019 |
| Gall Robertson Ltd | Professional & scientific | 2012 |
| Ganesh's Physiotherapy Limited | Health & social care | 2025 |
| GB Employment Law Ltd | Professional & scientific | 2023 |
| GC Clinical Limited | Professional & scientific | 2023 |
| GET Imagination Ltd | Professional & scientific | 2024 |
| GKM Property Maintenance Limited | Construction | 2015 |
| Glen Contracting Limited | Agriculture & fishing | 2024 |
| Glendearg Storage Ltd | Administrative & support | 2024 |
| Gleneagles Lets Ltd | Hospitality | 2017 |
| Glenfield Whisky Limited | Wholesale & retail | 2019 |
| Global Sports Innovation Ltd | Professional & scientific | 2011 |
| Goat Willow Pottery CIC | Manufacturing | 2024 |