| Eurolingua Ltd | Wholesale & retail | 2006 |
| Evabode Developments Limited | Construction | 2022 |
| Evabode Group Limited | Financial services | 2022 |
| Evabode Lettings Ltd | Real estate | 2019 |
| Evabode Ltd | Real estate | 2016 |
| Evabode Property Ltd | Real estate | 2009 |
| Evets Education Consultancy Ltd | Education | 2025 |
| EVO EV Limited | Manufacturing | 2019 |
| Evolution Gym Ltd | Arts & recreation | 2012 |
| Exitvalue Limited | Information & communication | 1986 |
| Extra Mile Logistics Ltd | Transport & logistics | 2025 |
| Extruder Services Ltd. | Professional & scientific | 2014 |
| Fabhairco Essentials Ltd | Wholesale & retail | 2017 |
| Fancy Candy Ltd | Wholesale & retail | 2024 |
| FDN Property Limited | Real estate | 2020 |
| Fdscl Ltd | Professional & scientific | 2026 |
| Feather and Field Ltd | Hospitality | 2026 |
| Fedoraparty Ltd | Wholesale & retail | 2022 |
| FEE Solutions Limited | Financial services | 2011 |
| Feeding Gainsborough CIC | Hospitality | 2019 |
| Felix Business Ltd | Wholesale & retail | 2020 |
| Fenix Enterprises Ltd | Information & communication | 2022 |
| Ferry Farm Ltd | Wholesale & retail | 2023 |
| Fiducia Physiotherapy and Aesthetics Ltd | Health & social care | 2018 |
| Fina Engineering Limited | Manufacturing | 2025 |
| Fina Welding Fabrications Ltd | Construction | 2015 |
| Firecycle Fire Safety Training Ltd | Professional & scientific | 2022 |
| First Choice Glazing Scunthorpe Ltd | Construction | 2025 |
| First Coho Limited | Financial services | 2025 |
| First Cone (Traffic Management) Ltd | Administrative & support | 2019 |
| Fisher Commercial Management Limited | Professional & scientific | 2014 |
| Fitness Consultancy Limited | Professional & scientific | 2025 |
| Five Star Crew Co. Ltd | Administrative & support | 2015 |
| Five Star Enterprise Group Ltd | Professional & scientific | 2018 |
| Five Star Phoenix Limited | Real estate | 2019 |
| Flat Floor Consulting Limited | Professional & scientific | 2012 |
| Flatdog UK Limited | Manufacturing | 2008 |
| Flexi PAK Limited | Manufacturing | 2009 |
| Flexipod Ltd | Manufacturing | 2026 |
| Flickity Limited | Health & social care | 2012 |
| Flip and Shine Ltd | Administrative & support | 2024 |
| Flood Management Support Services Limited | Professional & scientific | 2007 |
| FMB Fitness for ALL Limited | Other services | 2019 |
| Foma Real Estate Limited | Financial services | 2023 |
| Foolproof Construction Ltd | Construction | 2013 |
| FOR Giants Ltd | Professional & scientific | 2020 |
| Fordbridge MOT & Service Centre Ltd | Wholesale & retail | 2025 |
| Foreman & Co. Limited | Professional & scientific | 2008 |
| Forestry and Landbased Training Limited | Education | 2012 |
| Fortm Developments Limited | Construction | 2025 |
| Forty Winks of Lincoln Limited | Wholesale & retail | 2006 |
| Foster Construction Design Ltd | Construction | 2025 |
| Fourjays Property Ltd | Real estate | 2023 |
| FOX & Hounds Country Inn Limited | Hospitality | 2008 |
| FOX Investments Willingham Limited | Other services | 2021 |
| Foxbox Bar Ltd | Hospitality | 2023 |
| Franco Boxing CIC | Education | 2025 |
| Franco's Boxing Club Ltd | Arts & recreation | 2022 |
| Francos Properties Limited | Real estate | 2026 |
| Frankish Psychological Services Limited | Health & social care | 2008 |
| Frankish Training Limited | Health & social care | 2008 |
| Franklin Haber Ltd | Professional & scientific | 2005 |
| Freightmaster Blending & Warehousing Limited | Transport & logistics | 2015 |
| Freightmaster Limited | Transport & logistics | 2001 |
| Frost Joinery Ltd | Construction | 2026 |
| Frosty Properties Limited | Real estate | 2021 |
| Frozen Dream Holdings Ltd | Financial services | 2024 |
| FSC Training Ltd | Administrative & support | 2018 |
| FSG Security Ltd | Administrative & support | 2019 |
| Fsmc Ltd | Administrative & support | 2017 |
| FU11 Send Ltd | Arts & recreation | 2025 |
| FUN Printz Limited | Wholesale & retail | 2017 |
| Future Comms Ltd | Information & communication | 2009 |
| Future Homecare Yorkshire Ltd | Construction | 2024 |
| FW Music Limited | Arts & recreation | 2019 |
| G & F Robinson Ltd | Transport & logistics | 2015 |
| G Access Solutions Limited | Administrative & support | 2015 |
| G Burnett Limited | Construction | 2016 |
| G W Lord (Gainsborough) Ltd | Other services | 2025 |
| G W W Cook Ltd | Manufacturing | 2025 |
| G&A Property Investment & Design Ltd | Real estate | 2024 |
| G.E.B. North East Limited | Real estate | 2023 |
| G.Payne(Chemists)Limited | Health & social care | 1959 |
| G.R.Beeby & SON Limited | Agriculture & fishing | 1950 |
| G.S. Godson Limited | Construction | 1998 |
| G1PPP Limited | Transport & logistics | 2014 |
| G2 Structural Limited | Construction | 2013 |
| GA Hygiene Services Ltd | Health & social care | 2022 |
| Gaelenix Solutions Ltd | Information & communication | 2024 |
| Gains Church Street Stores Limited | Wholesale & retail | 2026 |
| Gainsborough Athletics Club Community Interest Company | Arts & recreation | 2024 |
| Gainsborough Building Services Limited | Construction | 2011 |
| Gainsborough Care Ltd | Health & social care | 2013 |
| Gainsborough Carpets (2013) Limited | Wholesale & retail | 2013 |
| Gainsborough Electrical Contractors Ltd | Construction | 2015 |
| Gainsborough Feral Rescue CIC | Agriculture & fishing | 2026 |
| Gainsborough Furniture & Resource Centre Community Interest Company | Water & waste | 2012 |
| Gainsborough Giftware Limited | Wholesale & retail | 2010 |
| Gainsborough Housing Ltd | Real estate | 2025 |
| Gainsborough Industrial Controls Limited | Manufacturing | 1984 |