| DOM Talbot Limited | Information & communication | 2018 |
| Down to Earth Agriculture Limited | Administrative & support | 2021 |
| Down to Earth Recycling Holdings Limited | Financial services | 2020 |
| Down to Earth Recycling Ltd. | Water & waste | 2006 |
| DR Jacques & SON Limited | Agriculture & fishing | 2020 |
| DRB Brickwork Limited | Construction | 2011 |
| Drewery & Wheeldon Estate Agents Ltd | Real estate | 2009 |
| Drewery and Wheeldon Auctioneers Ltd | Other services | 2015 |
| Drop&Go DRY Cleaners and Laundrette Ltd | Other services | 2018 |
| Drumbeat Collective Ltd | Other services | 2018 |
| Drumbeat Solutions Ltd | Professional & scientific | 2025 |
| Drury Building Services Limited | Construction | 2024 |
| Dsmerchants Ltd | Wholesale & retail | 2017 |
| Dtec Electrical Services Ltd | Construction | 2026 |
| Durastone Resin Ltd | Administrative & support | 2025 |
| Dusty Enterprises Ltd | Wholesale & retail | 2024 |
| DV Residential Ltd | Real estate | 2024 |
| Dwell Property Lettings Limited | Real estate | 2023 |
| E a Hemingway Developments Ltd | Construction | 2026 |
| E Hendrie Ltd | Other services | 2022 |
| E J Electricians Limited | Other services | 2019 |
| E P Court Limited | Financial services | 2018 |
| E.H. Advertising Limited | Professional & scientific | 1983 |
| EA Gusto Ltd | Hospitality | 2025 |
| Eagre Coaches Limited | Transport & logistics | 2002 |
| East England Co Limited | Construction | 2017 |
| East Ferry Timber Limited | Wholesale & retail | 2006 |
| East Hudson Developments Limited | Construction | 2001 |
| East Midlands Association of Motor Clubs Limited | Arts & recreation | 1998 |
| East Midlands Holdings Limited | Professional & scientific | 1996 |
| East Midlands Instrument Company Limited | Construction | 1996 |
| Eastern Air Executive Limited | Transport & logistics | 1980 |
| Easters & SON Commercial Services Limited | Wholesale & retail | 2016 |
| EASY-V0YAGE Limited | Administrative & support | 2025 |
| Ebim Ltd | Professional & scientific | 2021 |
| Ebony Jayne Properties Ltd | Real estate | 2025 |
| Ecbss Limited | Construction | 2013 |
| ECC Services (Lincs) Ltd | Administrative & support | 2025 |
| Echo Skincare Ltd | Wholesale & retail | 2015 |
| Ecoframe Installations Ltd | Construction | 2026 |
| Ecopro Engines Ltd | Wholesale & retail | 2026 |
| Ecoscheme Limited | Extraterritorial | 2011 |
| ED Morris Polo Ltd | Education | 2023 |
| EDB Electrical Services Ltd | Construction | 2026 |
| EFW Commercials Ltd | Transport & logistics | 2020 |
| EFW Transport Ltd | Transport & logistics | 2020 |
| Eighty Five Holdings Limited | Financial services | 2023 |
| EJP Consultants Ltd | Professional & scientific | 2022 |
| EKB Hairdressing Soul Limited | Other services | 2020 |
| EL Logistic Services Ltd | Transport & logistics | 2021 |
| Elach Indian Ltd | Hospitality | 2024 |
| Elachi Indian Ltd | Hospitality | 2026 |
| Elective Education Ltd | Education | 2025 |
| Electric Motor Sales and Service Limited | Manufacturing | 2023 |
| Electric Motor Site Services Limited | Manufacturing | 2015 |
| Electro Design LAB Ltd | Professional & scientific | 2025 |
| Elemental Landscapes Ltd | Administrative & support | 2022 |
| Elevate+ Ltd | Health & social care | 2025 |
| Elevation Health Limited | Health & social care | 2026 |
| Elilocks Hair Extensions Limited | Other services | 2026 |
| Elite Linings Limited | Construction | 2024 |
| Elite Pest Management Limited | Administrative & support | 2009 |
| Elite Renovations & Developments Ltd | Construction | 2025 |
| Elite Signs Limited | Manufacturing | 2003 |
| Elkins Electrical Services Ltd | Construction | 2014 |
| Ellers Farm Limited | Agriculture & fishing | 2023 |
| Elliott & Co. Joinery Ltd | Construction | 2016 |
| ELM Cottage (Gainsborough) Limited | Hospitality | 2013 |
| Elmfield Landscapes Limited | Administrative & support | 2014 |
| Elois Fine Food Ltd | Manufacturing | 2026 |
| Elwess Limited | Agriculture & fishing | 2025 |
| Elysian & Caim Ltd | Manufacturing | 2020 |
| Elysian Energy Group Ltd | Construction | 2024 |
| Elysian Healthcare and Aesthetics Ltd | Health & social care | 2023 |
| EM Dash CAM & Trackers Ltd | Wholesale & retail | 2025 |
| Embrace Inspire Support & Care Services Ltd | Health & social care | 2025 |
| Emic Group Limited | Professional & scientific | 2015 |
| Emily Cooper Racing Ltd | Other services | 2022 |
| Emily L Jones Limited | Professional & scientific | 2024 |
| Eminox Limited | Manufacturing | 1978 |
| Eminox New Ventures Limited | Manufacturing | 2002 |
| Emmeline Complex Care Limited | Health & social care | 2025 |
| Emmerald Polymers Limited | Extraterritorial | 2017 |
| Emmerson Pool Hire Ltd | Arts & recreation | 2024 |
| Emmerson Property Ltd | Real estate | 2024 |
| Emmerson Swim School Limited | Education | 2021 |
| Emmtech Electrical Ltd | Construction | 2026 |
| Emzies Shop Limited | Wholesale & retail | 2026 |
| Encore Beats Ltd | Information & communication | 2020 |
| Endopat Limited | Health & social care | 2020 |
| Endurance Trustee Limited | Extraterritorial | 2024 |
| Enertrustassociates Ltd | Professional & scientific | 2025 |
| ENRICH4 Ltd | Professional & scientific | 2016 |
| Entelechy Music Ltd | Information & communication | 2024 |
| Equestris Consultancy Ltd | Professional & scientific | 2026 |
| Erfurt UK Limited | Wholesale & retail | 2004 |
| Eric Food Express Ltd | Wholesale & retail | 2016 |
| Eryuz Limited | Hospitality | 2025 |
| Etak Steel Limited | Wholesale & retail | 2008 |
| Eukca Ltd | Real estate | 2014 |