| Heating & Detection Solutions Limited | Professional & scientific | 2006 |
| Henry Sheach Lawnmower Services Limited | Wholesale & retail | 2003 |
| Hilleraye Poultry | | 1999 |
| HMR Stores Ltd. | Wholesale & retail | 2008 |
| HN Methil Limited | Wholesale & retail | 2013 |
| Hoas Limited | Manufacturing | 2004 |
| Hogg Property Company Limited | Real estate | 1989 |
| Hoggs of Fife Limited | Wholesale & retail | 1928 |
| Hogs Haven Golf Club Limited | Financial services | 2023 |
| Homarna Ecostore Limited | Real estate | 2003 |
| Howe of Fife Medical Practice LLP | | 2021 |
| Hqnq Limited | Hospitality | 2007 |
| Hugh Farquhar Limited | Arts & recreation | 2002 |
| Hush Skin Limited | Other services | 2025 |
| I Q Interiors (Scotland) Limited | Wholesale & retail | 2008 |
| ICR Veterinary Group Ltd | Extraterritorial | 2008 |
| IN Balance Book-Keeping Limited | Professional & scientific | 2007 |
| India Lloyd Limited | Real estate | 1999 |
| Inglis Veterinary Centres Limited | Extraterritorial | 2008 |
| Inputduel Limited | Professional & scientific | 2003 |
| Intu IT (SCO) Ltd | Administrative & support | 2014 |
| J G Mccaw | | 1988 |
| J Y Thomson Limited | Manufacturing | 2003 |
| J. & J. Harley Ltd. | Agriculture & fishing | 1997 |
| J. G. ORR Limited | Agriculture & fishing | 1999 |
| J.G. Builders (Braidleys) Limited | Construction | 2002 |
| Jackson Accident Repair Centre Limited | Wholesale & retail | 2002 |
| James a. Scott (Cupar) Limited | Wholesale & retail | 1950 |
| James Hair Investments Limited | Other services | 1986 |
| James Mackie (Dundee) Limited | Wholesale & retail | 2003 |
| James Mcintyre & SON Limited | Construction | 2003 |
| James Pirie of ST Andrews Limited | Wholesale & retail | 2012 |
| Jamies Deliverys Ltd | Transport & logistics | 2025 |
| JBB Project Services Limited | Administrative & support | 2007 |
| Jd's Entertainments Ltd. | Arts & recreation | 2004 |
| JDP Construction Services Ltd | Administrative & support | 2007 |
| Jean Ferguson Ltd | Professional & scientific | 2002 |
| Jlaw Consultancy Limited | Information & communication | 2009 |
| JMR IT Solutions Limited | Manufacturing | 2006 |
| John Gordon Associates Limited | Professional & scientific | 2006 |
| John R. Graham Limited | Agriculture & fishing | 1960 |
| John White & SON (Weighing Machines) Limited | Manufacturing | 1972 |
| Johnstons of Wellfield | | 1977 |
| K2 Marketing Solutions Limited | Professional & scientific | 2002 |
| Kada Developments Ltd | Real estate | 2025 |
| Karnot Limited | Manufacturing | 2006 |
| Keirsbeath Farm Limited | Agriculture & fishing | 1953 |
| Kelly Contracts (Fife) Ltd. | Construction | 1997 |
| Kettle Produce Limited | Manufacturing | 1986 |
| Kilbrackmont Farms (1995) | | 1997 |
| Kilburns Farming Company Limited | Agriculture & fishing | 1975 |
| Kilrie Limited | Professional & scientific | 2018 |
| Kinaldy Farmers 2000 | | 2000 |
| Kincaple Farm Limited | Agriculture & fishing | 1974 |
| Kingdom CAD Management (Scotland) Limited | Professional & scientific | 2007 |
| Kingdom Care | | 1991 |
| Kingdom Hotels Limited | Hospitality | 2008 |
| Kingdom Taverns Group Limited | Professional & scientific | 2008 |
| Kingdom Taverns Limited | Hospitality | 2002 |
| Kingdom Technical Services Limited | Professional & scientific | 1982 |
| Kinghorn Fortune House Limited | Hospitality | 2018 |
| Kinneston Bloodstock Limited | Agriculture & fishing | 2006 |
| Kinniker Ltd. | Professional & scientific | 2005 |
| Kirkcaldy and District Motor Club Limited (the) | Arts & recreation | 1953 |
| Kirkcaldy Garden Centre Limited | Wholesale & retail | 1999 |
| Knockhill Holdings Limited | Arts & recreation | 2019 |
| Kohler Scotland Limited | Hospitality | 2004 |
| Kopalacouriers Ltd | Transport & logistics | 2023 |
| L.D. & J.L.C. Drysdale Limited | Real estate | 1949 |
| Ladybank Golf Club Limited | Arts & recreation | 2022 |
| Lamond & Murray Limited | Manufacturing | 1981 |
| Landdale Limited | Construction | 2005 |
| Lathockar Farmers | | 1987 |
| Law & Hamilton Plant & Equipment Limited | Manufacturing | 1996 |
| LD Ovenstone Ltd. | Construction | 2007 |
| Leggett CDT Ltd | Health & social care | 2026 |
| Lewis Commercials Ltd. | Wholesale & retail | 2005 |
| Lindsey Adam Limited | Wholesale & retail | 1989 |
| Links Golf Tours ST. Andrews Limited | Administrative & support | 1997 |
| Links Golf Travel Limited | Administrative & support | 1997 |
| Links Licensed Grocers Ltd. | Wholesale & retail | 2007 |
| Liquid GAS Equipment Limited | Extraterritorial | 2012 |
| Lisle Design Limited | Manufacturing | 2006 |
| Little Raith Farms | | 1990 |
| Living Water Ecosystems Ltd. | Water & waste | 1997 |
| Lomond Electrical Limited | Professional & scientific | 1997 |
| Longloch Farm Ltd | Agriculture & fishing | 2005 |
| Longloch Farming Ltd | Agriculture & fishing | 1992 |
| Looppack Limited | Real estate | 2002 |
| Lordscairnie Stud | | 1995 |
| Lundin Links Pharmacy Limited | Health & social care | 2003 |
| M.A. Hoggan (Joiners) Limited | Real estate | 2002 |
| Mac's Jewellers Ltd | Wholesale & retail | 2024 |
| Macgregor Limited | Professional & scientific | 2006 |
| Mackinnon Stonemasons Ltd | Other services | 2019 |
| Macmerry Pet and Horse Centre Limited | Extraterritorial | 2011 |
| Magma Fabrication (Glenrothes) Limited | Manufacturing | 1979 |
| Mainbrace Marine Limited | Manufacturing | 2001 |
| Mark SEY Enterprises Limited | Administrative & support | 1997 |
| MBM Vets Limited | Extraterritorial | 2014 |