| Coolforce Limited | Manufacturing | 2000 |
| Corps Properties 1664 Limited | Real estate | 2017 |
| Covemanor Limited | Professional & scientific | 2019 |
| Cowdenbeath Football Club, Ltd. (the) | Arts & recreation | 1905 |
| CR Smith Home Improvements Limited | Extraterritorial | 1993 |
| Craigsanquhar House & Country Estate Limited | Hospitality | 1996 |
| Creos Limited | Manufacturing | 1988 |
| Crown Associates GB Limited | Hospitality | 2000 |
| Cults Farm Partnerships | | 1987 |
| Cupar Bearings & Transmissions Limited | Wholesale & retail | 1986 |
| Cupar Motorcycles Limited | Wholesale & retail | 1992 |
| Cusick Motors Limited | Wholesale & retail | 2003 |
| D & D Mathie Limited | Agriculture & fishing | 2006 |
| D & L Electrical Services Ltd. | Professional & scientific | 2007 |
| DAC Security Limited | Administrative & support | 2019 |
| Dalgety Joiners Limited | Construction | 2003 |
| Dalrymple (Construction) Limited | Construction | 1973 |
| Dancehammer Group Limited | Professional & scientific | 2002 |
| David Beat Plumbing and Heating Limited | Construction | 2004 |
| Drumgolf Tours Limited | Arts & recreation | 2002 |
| Dunvegan Hotel Ltd. | Hospitality | 2003 |
| Dynamic Woods - Scottish Wood Limited | Manufacturing | 2000 |
| EAP Financial Ltd | Professional & scientific | 2019 |
| East Newton Farm | | 1994 |
| East Pier Smokehouse Ltd. | Hospitality | 2017 |
| Easter Ross Vets Limited | Extraterritorial | 2014 |
| Easyreach Access Hire Limited | Extraterritorial | 2007 |
| Ecodyn Limited | Professional & scientific | 2000 |
| Ecom Scotland Limited | Information & communication | 1999 |
| Edenwood Farms Limited | Agriculture & fishing | 1998 |
| Electrical and Energy Management Solutions Ltd | Administrative & support | 2007 |
| Elie Retailing Limited | Wholesale & retail | 2003 |
| Elliot Clays Ltd | Arts & recreation | 2023 |
| Elysium Homes (Scotland) Limited | Construction | 2021 |
| Engineering Specialised Support Services Ltd | Professional & scientific | 2020 |
| Eurojet (Scotland) Ltd. | Water & waste | 1999 |
| F.E.S. (Distribution) Limited | Transport & logistics | 1986 |
| Fairfield Care Services Ltd | Health & social care | 2023 |
| Fairfield Farms (1995) | | 1997 |
| Ferguson Manpower Limited | Professional & scientific | 2001 |
| Fife Construction Ltd | Construction | 2016 |
| Fife Fabrications Limited | Manufacturing | 1972 |
| Fife Flatterers Limited | Real estate | 1998 |
| Fife Inns (Cupar) Limited | Hospitality | 1989 |
| Fife Tractors Limited | Wholesale & retail | 1971 |
| Fife Traders Ltd | Hospitality | 2023 |
| Filmgold Limited | Information & communication | 2003 |
| Firesafe Consultancy Limited | Public administration | 2003 |
| Firth Buildings (Scotland) Limited | Manufacturing | 1977 |
| Flemings Furniture & Antiques Centre Limited | Wholesale & retail | 2002 |
| Flemings of Rosyth Limited | Transport & logistics | 2002 |
| Fondescence Ltd | Information & communication | 2026 |
| Foodie Farm Holdings Limited | Agriculture & fishing | 2020 |
| Forth Pilgrim! Ltd | Education | 2007 |
| Forth Valley Vets Limited | Extraterritorial | 2013 |
| Forthbay Commerce Limited | Information & communication | 2003 |
| Forthside Construction Limited | Construction | 1985 |
| Frank Munro & Sons Limited | Wholesale & retail | 2002 |
| Fraser Figures Limited | Administrative & support | 2002 |
| Fraser Kotlewski ( Joinery ) Limited | Construction | 2004 |
| Freshfocal Limited | Extraterritorial | 2002 |
| FTV Proclad (U.K.) Limited | Manufacturing | 2004 |
| Furniture Plus Limited | Water & waste | 2001 |
| Fusion Polymers Ltd | Manufacturing | 2023 |
| G G Graphics Limited | Information & communication | 2000 |
| G Henderson & Sons | | 2006 |
| G.S. Brown (Precision Engineers) Limited | Manufacturing | 1992 |
| G.S.C.C. Limited | Other services | 2002 |
| Gemcycle Limited | Construction | 2006 |
| Geodoor Limited | Other services | 2001 |
| George Laing (Fencing) Limited | Manufacturing | 1981 |
| Geyer Plant Services Limited | Manufacturing | 1978 |
| Glenburnie Care Ltd. | Health & social care | 2001 |
| Glenrothes Fabrications (Holdings) Limited | Professional & scientific | 2005 |
| Glenrothes Fabrications Ltd. | Manufacturing | 1999 |
| Glentarkie Farm | | 1994 |
| Goatmilk Farm | | 1994 |
| Goatmilk Farm | | 1998 |
| Golf Resorts International Limited | Administrative & support | 1989 |
| Goliath Strong Ltd | Arts & recreation | 2024 |
| Good News (Cowdenbeath) Ltd. | Wholesale & retail | 2004 |
| Good News (Dunfermline) Limited | Real estate | 1996 |
| Grange Garage (Fife) Limited | Wholesale & retail | 2001 |
| Graphic Clinic Ltd | Information & communication | 2005 |
| Greatmaker Ltd | Professional & scientific | 2023 |
| Greenmyre Mushroom Farm Limited | Real estate | 1978 |
| Greenside Agricultural Services Limited | Manufacturing | 1989 |
| Greer Holdings Limited | Financial services | 2021 |
| GS Landscapes (Fife) Ltd | Administrative & support | 2016 |
| Gudrun Sjoden Limited | Wholesale & retail | 2006 |
| H.P.G Properties Limited | Real estate | 2005 |
| HAK Properties Limited | Real estate | 2000 |
| Halbeath Farm (Holdings) Limited | Financial services | 1996 |
| Hamilton Computer Services Limited | Other services | 2005 |
| Hamiltoons Ltd | Arts & recreation | 2020 |
| Hampad Limited | Construction | 1981 |
| Harvester Homes (Fife) Limited | Construction | 2000 |
| Hatton (1988) Partnership | | 1989 |
| Heartland Veterinary Services Limited | Professional & scientific | 2010 |
| Heatherinch Farms | | 1992 |