| A Plus Brophy Construction Ltd | Construction | 2021 |
| A. Nicol & Company (Holdings) Limited | Professional & scientific | 1963 |
| A.& J.Nelson(Haulage Contractors)Limited | Transport & logistics | 1975 |
| A1 Minibus & Coach Services Ltd. | Transport & logistics | 1995 |
| A2 Carwash and Valeting Services Fife Ltd | Administrative & support | 2021 |
| AB Management Services Limited | Transport & logistics | 1998 |
| Abacus Financial Planning Consultants Limited | Financial services | 2003 |
| Abbeyford Caravan Company (Scotland) Limited | Hospitality | 1984 |
| Aberdeen Veterinary Referrals Limited | Extraterritorial | 2015 |
| Accounting Services Fife Ltd | Professional & scientific | 2004 |
| Adam Neilson Limited | Construction | 1976 |
| AI 3 Ltd | Wholesale & retail | 2022 |
| AIM Energy Limited | Professional & scientific | 2008 |
| Akre Limited | Agriculture & fishing | 2020 |
| Akre Systems Limited | Agriculture & fishing | 2017 |
| Alba Entertainments Limited | Arts & recreation | 2007 |
| Alexander & Ward Roofing Limited | Wholesale & retail | 1973 |
| Alexander Thomson & Company (Plumbers) Limited | Wholesale & retail | 1943 |
| Alistair Buchan Ltd. | Wholesale & retail | 2003 |
| Allan Farms | | 1996 |
| Allanhill Farming Co. Limited | Administrative & support | 1995 |
| Alttitude (2005) Limited | Professional & scientific | 2005 |
| AMI Lets Limited | Real estate | 2025 |
| Andrew Cook (Containers) Limited | Water & waste | 1990 |
| Andrew Cook (Contractors) Ltd. | Construction | 2006 |
| Andrew Elliot Cars Ltd | Wholesale & retail | 2026 |
| Andrew Hutt Limited | Wholesale & retail | 1974 |
| Andrew K. Price Limited | Professional & scientific | 2007 |
| Angle Park Sand and Gravel Company Limited | Mining & quarrying | 1978 |
| Apiu Global Ltd | Education | 2005 |
| Arcra Services Limited | Information & communication | 2016 |
| Ardene House VET Practice Ltd | Extraterritorial | 2011 |
| Ardessie Salmon Limited | Agriculture & fishing | 1986 |
| Arid Systems Limited | Information & communication | 2007 |
| Ashbank Holdings Ltd | Extraterritorial | 2017 |
| Ashbank VET Centre Ltd | Professional & scientific | 2014 |
| Ashford Farms and Estates Limited | Agriculture & fishing | 2015 |
| Ashford Renewables Limited | Construction | 2015 |
| Ashnor Investments Ltd | Transport & logistics | 2020 |
| Auld Kirk Charitable Trust | Arts & recreation | 2006 |
| Autopaint Fife Limited | Wholesale & retail | 2005 |
| Autoweld Limited | Wholesale & retail | 2002 |
| Avondale Veterinary Services Limited | Extraterritorial | 2012 |
| AX Ambassador Ltd | Professional & scientific | 2016 |
| Axyz Engineering Limited | Manufacturing | 2001 |
| B3 Environmental Limited | Agriculture & fishing | 2005 |
| Banchory Farms | | 1996 |
| Bargo Ltd | Hospitality | 2026 |
| Barkeldy Equestrian Limited | Other services | 2017 |
| Barroway Poultry | | 1994 |
| Bear Design (Scotland) Limited | Real estate | 2005 |
| Beard Property Limited | Real estate | 2025 |
| BEI XIN Limited | Hospitality | 2019 |
| Beinn Ratha Engineering Limited | Manufacturing | 2002 |
| Bell Surveys Limited | Construction | 2002 |
| Bennett Carpets Limited | Wholesale & retail | 2000 |
| Best Tanks Limited | Manufacturing | 2009 |
| Birch Leaf (Europe) Limited | Households | 2003 |
| Bitwise Group Limited | Wholesale & retail | 1996 |
| Bitwise Limited | Information & communication | 1987 |
| BJD (Fife) Ltd. | Professional & scientific | 2007 |
| Blackrock Roofing Ltd | Administrative & support | 2016 |
| Bliss (Scotland) Limited | Other services | 1999 |
| Bm&Pv Ltd | Transport & logistics | 2023 |
| Bouprie Banks Farm Limited | Agriculture & fishing | 2009 |
| Braefield Limited | Real estate | 1995 |
| Bridge Systems Limited | Information & communication | 1996 |
| Broomhead Garage Limited | Wholesale & retail | 2004 |
| Brucespeed Limited | Manufacturing | 2003 |
| Bszk Investment Ltd | Real estate | 2024 |
| Buckhaven Carpet Warehouse Limited | Wholesale & retail | 1973 |
| C & F Electrical Services Limited | Construction | 1998 |
| C Cunningham Inspections Ltd | Professional & scientific | 2020 |
| C R Smith Conservatories Limited | Extraterritorial | 1984 |
| Cadenergy Property Ltd. | Construction | 1990 |
| Cadscot Limited | Information & communication | 1999 |
| Caledonian Agricultural Management Services Limited | Administrative & support | 1979 |
| Calsay Poultry | | 1997 |
| Campbell & GAY (Builders) Limited | Construction | 2001 |
| Campion Homes Holdings Limited | Extraterritorial | 1989 |
| Campion Homes Limited | Construction | 2003 |
| Canmore Business Services Limited | Professional & scientific | 2007 |
| Canopus Media Limited | Information & communication | 2019 |
| Carnbo Clinical Research Limited | Professional & scientific | 2002 |
| CDL Vehicle Information Services Limited | Information & communication | 2004 |
| Central Belt Properties Ltd. | Construction | 2001 |
| Central Tail Lift Services Limited | Other services | 2004 |
| Centre Line Solutions Ltd. | Professional & scientific | 1998 |
| Ceres Fruits | | 2001 |
| CHA Fang Ltd | Hospitality | 2024 |
| Clan Lands of Scotland Limited | Information & communication | 1997 |
| Classic Autosports Limited | Wholesale & retail | 2000 |
| Clatto Barns Farmers L.P. | | 2002 |
| Clickstock Limited | Real estate | 2002 |
| Cloud 9 Accounting Experts Ltd | Professional & scientific | 2017 |
| Cloud 9 Property Solutions Ltd | Wholesale & retail | 2022 |
| Clushgreen Farms | | 1987 |
| Colin Thomson & Co. Limited | Professional & scientific | 1997 |
| Connect Adapt Thrive Ltd | Administrative & support | 2022 |
| Control Driving.Com Limited | Other services | 2003 |