| ELY Specsavers Hearcare Limited | Wholesale & retail | 2017 |
| ELY Specsavers Limited | Wholesale & retail | 2002 |
| Emac Limited | Other services | 1996 |
| Embedded IT Limited | Information & communication | 2014 |
| Embrace CX Ltd | Information & communication | 2018 |
| Emerald Automotive Ltd | Wholesale & retail | 2026 |
| Emerge Development Consultancy Group Limited | Financial services | 2024 |
| Emerge Development Consultancy Limited | Administrative & support | 1997 |
| Emersons Green Specsavers Limited | Wholesale & retail | 2018 |
| EMF Neif I (a) L.P. | | 2012 |
| EMF Neif I (B) L.P. | | 2012 |
| EMG Analytics Ltd | Information & communication | 2018 |
| Emir Stubington Ltd | Hospitality | 2025 |
| EMJ Electrical Contractors Limited | Construction | 2012 |
| Emlea Cleaning Services Ltd | Administrative & support | 2026 |
| Empire 13 Ltd | Hospitality | 2022 |
| Employment Advice Line Ltd | Administrative & support | 2024 |
| Empower ME Training Ltd | Education | 2020 |
| Empower New Energy Limited | Administrative & support | 2022 |
| Empoweru Solutions Ltd | Information & communication | 2025 |
| Empress Property Investment (Southern) Ltd | Real estate | 2024 |
| Empressive Ltd | Administrative & support | 2023 |
| EMS Friendly Farm Limited | Other services | 2022 |
| Emsa Training Ltd | Education | 2023 |
| EMZ Events Ltd | Professional & scientific | 2017 |
| Emzi Brand Limited | Administrative & support | 2012 |
| Emzo Marketing Limited | Professional & scientific | 2015 |
| Em’S Dog Grooming Ltd | Other services | 2024 |
| ENA Behaviour Consultancy Limited | Health & social care | 2025 |
| Enchanted Trees Limited | Administrative & support | 2012 |
| Encompass Geospatial Ltd | Professional & scientific | 2009 |
| Encompass Surveys Limited | Professional & scientific | 2024 |
| Encouraged Construction Ltd | Construction | 2019 |
| END Tackle Limited | Wholesale & retail | 2023 |
| Endcroft Limited | Professional & scientific | 2010 |
| Endeavour Business Park Management Company Limited | Real estate | 2004 |
| Endraw Limited | Professional & scientific | 2008 |
| Energenix Engineering Ltd | Construction | 2024 |
| Energy Fitness Professionals Limited | Arts & recreation | 2001 |
| Energy Report Limited | Professional & scientific | 2007 |
| Energy Systems Management Ltd | Other services | 2018 |
| Energy Your Way Ltd | Construction | 2023 |
| Enerveo Limited | Construction | 1988 |
| Enfield Crown Road Specsavers Limited | Wholesale & retail | 2018 |
| Enfield Specsavers Hearcare Limited | Wholesale & retail | 2006 |
| Enfield Specsavers Limited | Wholesale & retail | 1995 |
| Enfield Visionplus Limited | Wholesale & retail | 1995 |
| Enfields Franchising LLP | | 2006 |
| Engine Mode Ltd | Professional & scientific | 2022 |
| Engineered Applications Limited | Information & communication | 2015 |
| Enginuiti Ltd | Information & communication | 2024 |
| England Associates HR Consultancy Limited | Administrative & support | 2011 |
| Enhanced Home Property Ltd | Real estate | 2026 |
| Enhanced Power Services Limited | Information & communication | 2012 |
| Enix Limited | Information & communication | 2006 |
| Enliven Developments Ltd | Real estate | 2022 |
| Ensco NS Limited | Professional & scientific | 2004 |
| Ensign Estates (London) Limited | Real estate | 2015 |
| Enspire Group Ltd | Financial services | 2020 |
| Enspire Solutions Limited | Manufacturing | 2014 |
| Enviro Clean (Portsmouth) Limited | Administrative & support | 2023 |
| Enviro-Heat South Ltd | Construction | 2024 |
| Envirochem Analytical Laboratories Limited | Professional & scientific | 1989 |
| Envirochem Group Limited | Professional & scientific | 2025 |
| Environmental Sealand Services Limited | Extraterritorial | 1991 |
| Envirotecnic Limited | Wholesale & retail | 2011 |
| Envirowaste (Southern) Ltd | Extraterritorial | 2006 |
| Envolt Consulting Ltd | Professional & scientific | 2026 |
| Envy Hair Fareham Ltd | Other services | 2004 |
| Epaa Realty Ltd | Real estate | 2017 |
| EPF Newco 1 Limited | Administrative & support | 2016 |
| EPF Newco 2 Limited | Financial services | 2017 |
| Epping Specsavers Limited | Wholesale & retail | 2008 |
| Epping Visionplus Limited | Wholesale & retail | 2008 |
| EPR Limited | Professional & scientific | 2015 |
| Epsom Specsavers Hearcare Limited | Wholesale & retail | 2014 |
| Epsom Specsavers Limited | Wholesale & retail | 1986 |
| Epsom Visionplus Limited | Wholesale & retail | 1993 |
| Erikom Building Solutions Limited | Construction | 2014 |
| Erikom Scaffolding Solutions Limited | Construction | 2025 |
| Erith Specsavers Limited | Wholesale & retail | 2005 |
| Erith Visionplus Limited | Wholesale & retail | 2005 |
| ESI Marketing Ltd | Other services | 2019 |
| Eskimo 11 Limited | Arts & recreation | 2008 |
| Eskom Cars Limited | Construction | 2025 |
| Esla Feeder Fund | | 2003 |
| Esroe Limited | Information & communication | 2009 |
| Essex 2 Domiciliary Specsavers Limited | Wholesale & retail | 2014 |
| Estate Agency Franchise Group Ltd | Real estate | 2017 |
| Estates Consulting Hubb Ltd | Administrative & support | 2024 |
| Estatesco Derby Iron Gate Ltd | Real estate | 2019 |
| Estatesco Ltd | Financial services | 2014 |
| Estatesco Pontypridd Ltd | Real estate | 2019 |
| Estelle's Beauty and Asthetics Ltd | Other services | 2023 |
| Etali Limited | Information & communication | 2003 |
| Etherington's Estates Ltd | Construction | 2016 |
| Ethiceyes Limited | Administrative & support | 2018 |
| Ethnic Diamondz Ltd | Education | 2012 |
| Europa Furnishing Limited | Manufacturing | 1996 |
| European Fuel Solutions Limited | Wholesale & retail | 2007 |