| Rosehall Garage Limited | Wholesale & retail | 1982 |
| Ross Perry Ltd | Construction | 2016 |
| Rotech Motors & Pumps Ltd | Manufacturing | 2013 |
| Roubaix Developments Limited | Construction | 2020 |
| Rowan Beauty Studio Ltd | Other services | 2014 |
| RPM (Fife) Ltd. | Construction | 2008 |
| RPS Facilities Ltd | Administrative & support | 2016 |
| Rrrpropertygroup Ltd | Real estate | 2023 |
| RS Lubricants Ltd | Wholesale & retail | 2013 |
| RSH Holdings Ltd | Real estate | 2015 |
| RTB Construction Ltd | Construction | 2018 |
| RTS Glen Ltd | Wholesale & retail | 2018 |
| Ruban Rouge (Bofa) Limited | Wholesale & retail | 2011 |
| Ruedas Limited | Other services | 2025 |
| Rumford Car Wash Ltd | Administrative & support | 2018 |
| Rurali Limited | Other services | 2021 |
| Rush B Tech Limited | Information & communication | 2017 |
| Rush Beverages Ltd | Manufacturing | 2025 |
| Russel + Aitken (Falkirk + Alloa) Ltd | Professional & scientific | 2018 |
| Russel + Aitken LLP | | 2005 |
| Russel + Aitken Nominees Limited | Financial services | 1972 |
| Russell and Ralston Electrical Ltd | Construction | 2021 |
| RW Agencies Limited | Wholesale & retail | 2011 |
| RWY Automotive Ltd | Wholesale & retail | 2025 |
| Ryamlay Properties Ltd. | Real estate | 2021 |
| Ryan Offshore International Ltd | Mining & quarrying | 2023 |
| Ryler Automotive Ltd | Wholesale & retail | 2026 |
| S & C Joiners Limited | Construction | 2015 |
| S & I Builders Limited | Construction | 2017 |
| S & R Foods (Cumbernauld) Limited | Hospitality | 2020 |
| S & R Foods (Stirling) Limited | Hospitality | 2019 |
| S Mcmillan Plumbing and Heating Ltd | Construction | 2022 |
| S Young Properties Limited | Real estate | 2021 |
| S&J Vision Limited | Financial services | 2021 |
| S&R Foods (Cambuslang) Ltd | Hospitality | 2022 |
| S&R Foods (Coatbridge) Limited | Hospitality | 2024 |
| S&R Foods (Scotland) Limited | Hospitality | 2016 |
| S&S Partners Ltd | Wholesale & retail | 2025 |
| S.A.Y. Contracting Ltd. | Administrative & support | 2018 |
| Sabr Facilities Management Ltd | Administrative & support | 2024 |
| Safe Flight Solutions Ltd | Professional & scientific | 2024 |
| Safe Hands First AID Training Ltd | Education | 2015 |
| Safe Hands Health Ltd | Health & social care | 2023 |
| Safe Stay Registered Ltd | Financial services | 2024 |
| Safetech Quality and Inspection Services Limited | Professional & scientific | 2025 |
| Sailingfast Limited | Arts & recreation | 2017 |
| Saint Haven Group Ltd | Hospitality | 2026 |
| Salon Marshall Limited | Other services | 2023 |
| Saltire Project Solutions Ltd | Professional & scientific | 2025 |
| Salvage Operations Limited | Water & waste | 2024 |
| SAM K Logistics Ltd | Transport & logistics | 2025 |
| SAM Smith NDT Ltd | Professional & scientific | 2014 |
| Same ODD Thing Ltd | Arts & recreation | 2025 |
| Sammax Ground Works Limited | Construction | 2005 |
| Sandra Patterson Limited | Professional & scientific | 2025 |
| Sandy Law & SON Ltd. | Construction | 2024 |
| Sanguan Thai Therapy Ltd | Health & social care | 2024 |
| Sano Aspirations Ltd | Arts & recreation | 2023 |
| Sanq Energy & Services Ltd | Professional & scientific | 2019 |
| Sapphire Cheer and Dance CIC | Arts & recreation | 2024 |
| Saradale Consult Limited | Professional & scientific | 2023 |
| Saradale Limited | Professional & scientific | 2023 |
| Saraswoti Foods Limited | Hospitality | 2020 |
| Sardars Cellar Ltd | Wholesale & retail | 2024 |
| Sardars Properties Ltd | Households | 2024 |
| Satalite 2 Ltd | Other services | 2024 |
| Satori Mind Limited | Education | 2022 |
| Saville Contractors Limited | Construction | 2009 |
| Savory Sprint Ltd | Hospitality | 2024 |
| SBG Joinery and Building Ltd | Construction | 2013 |
| SBS Accountancy Ltd | Professional & scientific | 2015 |
| SC Automotive Ltd | Manufacturing | 2025 |
| SC Digital Media Limited | Information & communication | 2026 |
| SC Subsea Ltd | Professional & scientific | 2017 |
| Sc&D (Scotland) Ltd. | Arts & recreation | 2025 |
| Scarlet Ribbons (Falkirk) Ltd | Wholesale & retail | 2013 |
| SCC Automotive Ltd | Professional & scientific | 2025 |
| SCE Property Limited | Real estate | 2024 |
| SCF Scot Ltd | Hospitality | 2020 |
| Scival Ltd. | Information & communication | 2005 |
| Scot Properties Ltd | Real estate | 2024 |
| Scotia Contracts and Plant Ltd | Construction | 2026 |
| Scotincgrowth Ltd | Information & communication | 2019 |
| Scott & Ryan Logistics Ltd | Transport & logistics | 2023 |
| Scott Glass Ltd. | Manufacturing | 2008 |
| Scott Investments (Scotland) Limited | Professional & scientific | 2013 |
| Scotweigh Ltd. | Professional & scientific | 2014 |
| Screen Access Ltd | Education | 2026 |
| Scribli Ltd | Extraterritorial | 2023 |
| Script Coffee Limited | Hospitality | 2026 |
| Scylla Scot Ltd | Administrative & support | 2020 |
| SE Construction Services Ltd | Professional & scientific | 2025 |
| Seagull Cafe Ltd | Hospitality | 2025 |
| Seagull Enterprises Limited | Wholesale & retail | 2023 |
| Seagull Trust Cruises | Other services | 1997 |
| Sealco (Scotland) Limited | Construction | 1997 |
| Searching Scotland Limited | Administrative & support | 2016 |
| Seaview Millport Ltd | Real estate | 2023 |
| Seazoom Ltd | Information & communication | 2025 |
| Second Life Clothing Ltd | Wholesale & retail | 2024 |