| Reformer Club Fairford Limited | Arts & recreation | 2025 |
| Resintech Limited | Manufacturing | 1985 |
| Richard Stearn Limited | Construction | 2014 |
| Richards Engineering Services Ltd | Manufacturing | 2024 |
| Rock Stone Silk Ltd | Professional & scientific | 2017 |
| Roger's Natural Foods Ltd | Wholesale & retail | 2009 |
| Royal International Air Tattoo Flying Scholarships for Disabled People | Other services | 2012 |
| SAM Herman Studio Limited | Arts & recreation | 1981 |
| Savanah & Stone Properties Ltd | Real estate | 2025 |
| Seed House Creative Ltd | Professional & scientific | 2019 |
| Senin Ltd | Information & communication | 2023 |
| Servetape Limited | Information & communication | 1985 |
| Shalim Catering Ltd | Hospitality | 2024 |
| Shireside Playback Limited | Information & communication | 2021 |
| Shona Travel Limited | Administrative & support | 2022 |
| Snowdon Property Group Limited | Real estate | 2025 |
| Stable Cottage Classics Ltd | Wholesale & retail | 2024 |
| Staygo Campervan Hire Ltd | Administrative & support | 2022 |
| Stoneways North (Fairford) Management Company Limited | Real estate | 2016 |
| Stoneways South (Fairford) Management Company Limited | Real estate | 2016 |
| Straightlign Limited | Wholesale & retail | 2025 |
| Taos Limited | Wholesale & retail | 2006 |
| Tate & Tate Limited | Manufacturing | 2024 |
| THE Air Power Association Ltd | Other services | 2015 |
| THE Coarse Club Ltd. | Manufacturing | 2018 |
| THE Conditioning Club Ltd | Arts & recreation | 2021 |
| THE Cross Stitch Guild Limited | Manufacturing | 1995 |
| THE Fairford Cottage Limited | Real estate | 2023 |
| THE Family Osteopath Ltd | Health & social care | 2004 |
| THE Paperback Shop.Co.Uk Ltd | Professional & scientific | 2000 |
| THE Retreat Boarding Kennels Limited | Hospitality | 2020 |
| THE Royal Air Force Charitable Trust Enterprises | Wholesale & retail | 1987 |
| THE System DOT Cloud Limited | Information & communication | 2019 |
| THE Technology Team Limited | Information & communication | 2017 |
| THE Whitespace Gallery Ltd | Wholesale & retail | 2025 |
| Thryv Ltd | Construction | 2024 |
| Total Weed Control Limited | Administrative & support | 2024 |
| UK Dental Supplies Ltd | Manufacturing | 2018 |
| Uncover Therapeutics Limited | Professional & scientific | 2025 |
| Vanguard Art C.I.C. | Arts & recreation | 2019 |
| Village Tale Productions Ltd | Information & communication | 2022 |
| Volans Alte Ltd | Education | 2013 |
| Vortex Management Services Limited | Extraterritorial | 2019 |
| Walls Building & Groundwork Ltd | Construction | 2024 |
| Watercraft Management Limited | Administrative & support | 2012 |
| Western Centrica Limited | Wholesale & retail | 2023 |
| Wills Family Office Services Ltd | Administrative & support | 2026 |
| Wistmans Consultancy Services Ltd | Professional & scientific | 2025 |
| Woodward Engineering Ltd | Manufacturing | 2026 |
| Zintang Limited | Professional & scientific | 2017 |