| Farmor's School | Education | 2011 |
| FCP Football Ltd | Education | 2025 |
| FCP Suppliers Ltd | Construction | 2025 |
| Ff&K Ltd | Wholesale & retail | 2024 |
| FI Passey Consulting Limited | Professional & scientific | 2019 |
| First LAB Direct Limited | Manufacturing | 2005 |
| Flight Safety Awareness Limited | Transport & logistics | 2019 |
| Flying Start Nursery Limited | Education | 2010 |
| Foodfellas Catering Ltd | Hospitality | 2026 |
| Forty Five Clothing Ltd | Wholesale & retail | 2020 |
| Fourth Screen Consulting Limited | Information & communication | 2017 |
| Froma Ltd | Manufacturing | 2024 |
| G. Vitale Limited | Construction | 2003 |
| G.G. Loveridge Limited | Wholesale & retail | 1975 |
| Gango Group Ltd | Hospitality | 2023 |
| Gateway Communications Ltd | Information & communication | 2012 |
| Geotechnical Investigations Limited | Professional & scientific | 2012 |
| Global Trade Compliance Solutions Ltd | Professional & scientific | 2023 |
| Goodall Roofing Ltd | Construction | 2023 |
| Grayman Limited | Real estate | 2021 |
| Hannington Wood Ltd | Construction | 2010 |
| Hector & Queen Limited | Manufacturing | 2011 |
| Hello Muni Ltd | Wholesale & retail | 2020 |
| Heritage Roofing Services Ltd | Construction | 2015 |
| Hersom Whittaker Limited | Professional & scientific | 2010 |
| Hinder Building Services Limited | Construction | 2013 |
| Hocus Pocus Play Ltd | Arts & recreation | 2018 |
| Hodsons Associates Limited | Professional & scientific | 2006 |
| Hooves and Lenses Ltd | Professional & scientific | 2025 |
| Horcott Road Management Limited | Real estate | 2025 |
| Hurley Blue Limited | Professional & scientific | 2014 |
| Ibkc Ltd | Administrative & support | 2016 |
| IN2 Groundworks Ltd | Construction | 2020 |
| Increation Interiors Ltd | Construction | 2018 |
| Increation Limited | Construction | 2010 |
| Investing in the People of Tomorrow Ltd | Education | 2020 |
| J2H Investments Ltd | Financial services | 2026 |
| James Cotswold Property Ltd | Real estate | 2025 |
| Janoah Digital Ltd | Information & communication | 2016 |
| Japanese Knotweed Removal Limited | Other services | 2011 |
| JB Construction and Landscaping Limited | Construction | 2018 |
| Jbkc Group Ltd | Hospitality | 2024 |
| Jenners Barn Events Limited | Hospitality | 2022 |
| JIM Potts Ltd | Professional & scientific | 2023 |
| John Watson Holding Company Limited | Financial services | 2024 |
| JPA Financial Planning Limited | Professional & scientific | 2023 |
| Kaedyn. Ltd | Professional & scientific | 2024 |
| Katy Campbell Limited | Information & communication | 2018 |
| Kempsford Kiln Ltd | Manufacturing | 2023 |
| Kennedy Distilling Limited | Manufacturing | 2021 |
| KGB Medical Services Ltd | Health & social care | 2011 |
| Kgi(International Horse Transport)Ltd Ltd | Transport & logistics | 2019 |
| Kinexus Internet Limited | Information & communication | 2000 |
| KJ Businesses 4 Sale Ltd | Administrative & support | 2026 |
| KJ Properties and Land Limited | Real estate | 2018 |
| KJ Recruits Ltd | Administrative & support | 2026 |
| L & N Woodward Ltd | Construction | 2018 |
| Lane House Management Limited | Hospitality | 2015 |
| Lawes Limited | Real estate | 1985 |
| Liquid Knowledge Ltd | Wholesale & retail | 2024 |
| LSA Technical Resources Ltd | Wholesale & retail | 2022 |
| M & G Cotswolds Ltd | Construction | 2018 |
| M a Ponting Builders Limited | Construction | 2007 |
| MA Maison Belle Ltd | Wholesale & retail | 2025 |
| Magic Beans Cafe Ltd | Hospitality | 2018 |
| Main-Spar Aviation Ltd | Education | 2020 |
| Maklog Ltd | Transport & logistics | 2023 |
| Marnell Properties Limited | Real estate | 2016 |
| Mojos Clothing Limited | Wholesale & retail | 2014 |
| N&B GAS Guzzlers Ltd | Wholesale & retail | 2025 |
| Newland Portfolio Limited | Real estate | 2025 |
| Nissi Property Investments Ltd | Real estate | 2020 |
| Nosh! at the Cotswold Pudding Company Limited | Wholesale & retail | 2024 |
| NPK House Limited | Hospitality | 2025 |
| Ohworks Accident Repair and Paintwork Ltd | Wholesale & retail | 2018 |
| Otto Interiors and Workshop Limited | Manufacturing | 2024 |
| Partridge Accountancy Services Limited | Professional & scientific | 2016 |
| Paul Cornell Limited | Arts & recreation | 2003 |
| Pbshop.Co.Uk Limited | Wholesale & retail | 2014 |
| Performance Autoworks LLP | | 2011 |
| Perry House and Durie House Management Company Limited | Extraterritorial | 2023 |
| Pichiba Ltd | Real estate | 2023 |
| Pinks Barn LLP | | 2015 |
| Premier CRU Hotels & Inns LLP | | 2006 |
| Principal Imagery Limited | Professional & scientific | 2017 |
| PRL Young Ltd | Education | 2018 |
| Project 1 Bathrooms Ltd | Construction | 2023 |
| Project Kingfisher Ltd | Information & communication | 2009 |
| Purair Limited | Professional & scientific | 2017 |
| Pure Filth Food Limited | Hospitality | 2016 |
| Purely Divine Limited | Other services | 2015 |
| Q O T W Holdings Limited | Professional & scientific | 2016 |
| Quickfire Consultancy Limited | Public administration | 2026 |
| R J Contract Cleaning Limited | Other services | 2013 |
| R V B Limited | Hospitality | 2009 |
| Rathmead Limited | Professional & scientific | 2011 |
| Rc&I Ltd | Administrative & support | 2025 |
| RCL Storage Limited | Real estate | 2021 |
| Ready-To-Sell for YOU Limited | Professional & scientific | 2013 |
| Rees-Jones Consulting Ltd | Information & communication | 2022 |