| Ellie and BEA Limited | Wholesale & retail | 2018 |
| ELM Grove Holding Limited | Financial services | 2022 |
| Elmgrove Caravans Limited | Real estate | 1961 |
| Elmina Performance Ltd | Other services | 2021 |
| Elmleigh Owners Company Limited | Households | 2007 |
| Elsco Ltd | Arts & recreation | 2024 |
| Embers Wealth Management Ltd | Financial services | 2025 |
| Emery Oleochemicals UK Limited | Administrative & support | 2005 |
| Emhr Consulting Ltd | Professional & scientific | 2024 |
| Emily Jones Hair Ltd | Other services | 2019 |
| Emit-Uk Timing Limited | Arts & recreation | 2013 |
| Empire Contracting M&E Limited | Construction | 2018 |
| Empire Homes and Developments Ltd | Real estate | 2024 |
| Emporium Specialist Services Ltd | Wholesale & retail | 2014 |
| Empower T1D Ltd. | Arts & recreation | 1997 |
| Empowering Smiles Ltd | Health & social care | 2021 |
| Empowerment Engine C.I.C. | Professional & scientific | 2025 |
| Emsworth Property Ltd | Construction | 2018 |
| Enable Yourself Ltd | Construction | 2012 |
| END of INK Ltd | Health & social care | 2019 |
| Endoscopy Medics Limited | Health & social care | 2013 |
| Energitze Limited | Information & communication | 2016 |
| Energy Conservation Solutions Limited | Construction | 1998 |
| Energys Group Limited | Professional & scientific | 2006 |
| Enertric Technology Limited | Extraterritorial | 2005 |
| Enfields Eastleigh LLP | | 2009 |
| Engage in Their Future Ltd | Education | 2005 |
| Engage Talent Group Ltd | Administrative & support | 2026 |
| Engine Doctor Ltd | Manufacturing | 2016 |
| Engineering Component Resources Limited | Wholesale & retail | 2020 |
| Engineering Conferences Limited | Professional & scientific | 2003 |
| Engineering Constructions Limited | Professional & scientific | 2004 |
| Engineering Infinity Ltd | Professional & scientific | 2023 |
| Englabs Ltd | Manufacturing | 2019 |
| Engraving & Sign Solutions Ltd | Other services | 2011 |
| Enhance Power Ltd | Construction | 2015 |
| Enhanced Computer Solutions Trustee Company Limited | Professional & scientific | 2022 |
| Enhanced Studios Ltd | Other services | 2025 |
| Enigma Techno Ltd | Information & communication | 2024 |
| Enone Studio Ltd. | Information & communication | 2012 |
| Enpei Holdings Limited | Financial services | 2023 |
| Ensemble Penny Limited | Health & social care | 2023 |
| Enturprise Ltd | Real estate | 2022 |
| Enviro-Cold Services Limited | Manufacturing | 2019 |
| Enzo Design Studio Ltd | Professional & scientific | 2019 |
| Epeus Group Holdings Limited | Administrative & support | 2011 |
| Epi-V Equity Investments, L.P. | | 2011 |
| Epi-V Equity, L.P. | | 2007 |
| Equinable Limited | Health & social care | 2005 |
| Eric's Driving School Ltd | Education | 2025 |
| Erindom Ltd | Professional & scientific | 2018 |
| ERJ Enterprises Ltd | Real estate | 2024 |
| ERP Knight Limited | Information & communication | 2018 |
| Erpiq Limited | Information & communication | 2020 |
| Ersi Limited | Hospitality | 2020 |
| ES Storage Ltd | Wholesale & retail | 2025 |
| Escalator Handrail (UK) Limited | Administrative & support | 1998 |
| ESF Quality Ltd | Hospitality | 2005 |
| ESG Ethical Investments Limited | Financial services | 2019 |
| ESG Ethical Limited | Financial services | 2019 |
| Eshapeshaf Ltd | Professional & scientific | 2025 |
| Esjay Limited | Professional & scientific | 1998 |
| ESL Technical Services Limited | Administrative & support | 2020 |
| ESP IT Consultancy Ltd | Information & communication | 2008 |
| Esparda Ltd | Wholesale & retail | 2024 |
| Espastree Ltd | Manufacturing | 2025 |
| Essence in Motion Media Ltd | Administrative & support | 2024 |
| Essential Cleaning & Maintenance Limited | Professional & scientific | 2008 |
| Essentially Canterton Ltd | Professional & scientific | 2023 |
| ET Design Studio Limited | Information & communication | 2009 |
| ETC Football Limited | Education | 2019 |
| ETC HP Football Limited | Education | 2019 |
| Ethical Software Solutions Limited | Information & communication | 2025 |
| Ettinger Consulting Services Ltd | Professional & scientific | 2023 |
| Eunopharm Ltd | Information & communication | 2026 |
| Europa School of English Limited | Education | 2004 |
| EVA Logistics Transport Ltd | Transport & logistics | 2025 |
| Evaden Community Services Ltd | Health & social care | 2020 |
| Evaranks Ltd | Professional & scientific | 2023 |
| Eve's Bazaar Ltd | Wholesale & retail | 2013 |
| Evenbell Properties Limited | Real estate | 2013 |
| Event Bars South Limited | Hospitality | 2019 |
| Event Case Systems Ltd | Manufacturing | 2025 |
| Event Consulting Limited | Professional & scientific | 2017 |
| Event Electrical Ltd | Energy | 2010 |
| Event Electrical Systems Ltd | Administrative & support | 2010 |
| Event Interiors Limited | Manufacturing | 2002 |
| Evercommerce UK Company Limited | Financial services | 2019 |
| Everest Recruitment Limited | Administrative & support | 2008 |
| Everform Consulting Limited | Professional & scientific | 2025 |
| Everform Limited | Construction | 2023 |
| Evergreen Connections Ltd | Professional & scientific | 2025 |
| Evergreen Rabbit Control Ltd | Agriculture & fishing | 2016 |
| Everton Road Management Company Limited | Households | 2024 |
| Everyday Bites Ltd | Hospitality | 2022 |
| Evolution 4 Business Ltd | Administrative & support | 2013 |
| Evolution Global Executive Search Ltd | Administrative & support | 2009 |
| EWE EYE Limited | Information & communication | 2006 |
| Ewif Limited | Other services | 2010 |
| Ewshot Gardens Estate Ltd | Administrative & support | 2015 |