| East Walls (Chichester) Limited | Other services | 2013 |
| Eastleigh Accountants (Holdings) Limited | Professional & scientific | 2017 |
| Eastleigh Accountants Limited | Professional & scientific | 2008 |
| Eastleigh BID Ltd | Administrative & support | 2014 |
| Eastleigh Burger Trading Limited | Hospitality | 2019 |
| Eastleigh CAB Company Limited | Transport & logistics | 2012 |
| Eastleigh Carpets Limited | Wholesale & retail | 1987 |
| Eastleigh Contract Flooring Limited | Construction | 2010 |
| Eastleigh Fencing Supplies Ltd | Agriculture & fishing | 2010 |
| Eastleigh Football Club Community Trust | Arts & recreation | 2019 |
| Eastleigh Football Club Limited | Arts & recreation | 2005 |
| Eastleigh Holdings Ltd | Real estate | 2021 |
| Eastleigh Lakeside Railway Limited | Transport & logistics | 1995 |
| Eastleigh Masonic Centre Limited | Other services | 2021 |
| Eastleigh Osteopathic Clinic Limited | Health & social care | 1989 |
| Eastleigh Poppins Restaurant Ltd | Hospitality | 2023 |
| Eastleigh Sushi Ltd | Wholesale & retail | 2021 |
| Eastleigh Valeting Centre Ltd | Other services | 2017 |
| Eastleigh Wellbeing Centre Ltd | Health & social care | 2025 |
| Eastleigh Youth & Community Trust | Real estate | 2013 |
| Easy Clean Pro Solutions Ltd | Administrative & support | 2018 |
| Easy Start Design Ltd | Information & communication | 2020 |
| Easyobd Tools Ltd | Wholesale & retail | 2013 |
| Eatwell Properties Ltd | Real estate | 2023 |
| EAV Ltd | Real estate | 2024 |
| Eazhi Accounting Solutions Ltd | Professional & scientific | 2024 |
| EBM Roofing Limited | Construction | 2013 |
| Eccles Barfield Ltd | Construction | 2017 |
| Eccles Barly (Knowle) Ltd | Construction | 2025 |
| Eccles Building & Consultancy LLP | | 2015 |
| ECF Holdings Limited | Financial services | 2024 |
| Echo Brand Communications Limited | Manufacturing | 2014 |
| ECM Consultancy Limited | Real estate | 2024 |
| Eco Spray Insulations Limited | Construction | 2017 |
| Ecocompare Ltd | Administrative & support | 2026 |
| Ecoflame GAS and Heating Ltd | Construction | 2026 |
| Ecomm Growth Club Ltd | Wholesale & retail | 2025 |
| Ecommerce Supply Chain Ltd | Transport & logistics | 2017 |
| Economy Heating Services Limited | Construction | 2000 |
| Ecoolref Ltd | Construction | 2026 |
| Ecosysdynamics Limited | Information & communication | 2019 |
| Eden's Edge Landscaping Ltd | Administrative & support | 2025 |
| Edens Aces Properties Group Limited | Real estate | 2022 |
| Edge IT Services Ltd | Information & communication | 2017 |
| Edgecombes Ltd | Construction | 2007 |
| Edify Learning Ltd | Education | 2022 |
| Edinburgh Napier International College Limited | Education | 2023 |
| Edisto Limited | Professional & scientific | 2009 |
| Edit Monkey Limited | Extraterritorial | 2016 |
| EDN Solutions Ltd | Administrative & support | 2010 |
| Educas Limited | Education | 2008 |
| Education & Media Services Ltd | Education | 1998 |
| Edzio Consulting Limited | Information & communication | 2025 |
| EF Electrical Ltd | Energy | 2019 |
| EFC Eastleigh Limited | Hospitality | 2021 |
| EFC Solent Limited | Hospitality | 2019 |
| EH Consulting Limited | Professional & scientific | 2006 |
| Eica Installations Limited | Construction | 2023 |
| Eido Solutions Ltd | Professional & scientific | 2024 |
| Eidos Solutions Limited | Professional & scientific | 2025 |
| EJW Accountants Limited | Professional & scientific | 2021 |
| Ekhaya Impact Bucks Limited | Real estate | 2025 |
| Ekombiz Ltd | Wholesale & retail | 2019 |
| Elan Sales Limited | Wholesale & retail | 1987 |
| Elassy Derma-Plast Ltd | Health & social care | 2026 |
| Elbeck Management Ltd | Professional & scientific | 2020 |
| Eldridge Park Wokingham Management Ltd | Households | 2017 |
| Eldridge-Moss Limited | Professional & scientific | 2003 |
| Elecon UK Holdings Ltd | Financial services | 2022 |
| Elecon UK Limited | Wholesale & retail | 2007 |
| Electra Air Limited | Energy | 2022 |
| Electri-Click Limited | Extraterritorial | 2017 |
| Electric Digital Limited | Professional & scientific | 2012 |
| Electrical Leadership Services Limited | Construction | 2026 |
| Electrical Response Services Ltd | Construction | 2016 |
| Electricor Limited | Administrative & support | 2005 |
| Electrifire Limited | Administrative & support | 2009 |
| Electro Freeze Sales (U.K.) Limited | Professional & scientific | 1991 |
| Electroaircraft Ltd | Manufacturing | 2020 |
| Electronic Care Solutions Ltd | Manufacturing | 2019 |
| Electronics Machine Limited | Professional & scientific | 2006 |
| Electrostatic Solutions Limited | Professional & scientific | 1997 |
| Elektratex Engineering Limited | Construction | 2018 |
| Elephant Case Management & Training Solutions Limited | Education | 2025 |
| Elephant Enterprises Limited | Health & social care | 2022 |
| Elevare Talent Labs Ltd | Administrative & support | 2025 |
| Elevate Agency Online Limited | Other services | 2021 |
| ELEVATE74 Ltd | Other services | 2026 |
| Elevated Garden Services Ltd | Administrative & support | 2020 |
| Elgin Business Group Limited | Wholesale & retail | 2017 |
| Elite Cardiac Screening Ltd | Health & social care | 2019 |
| Elite Entrance Systems Limited | Construction | 2008 |
| Elite Executive Coaching Ltd | Health & social care | 2021 |
| Elite JOB Connect Ltd | Administrative & support | 2023 |
| Elite Kitchen Respray Ltd | Construction | 2026 |
| Elite Livein Care Limited | Health & social care | 2014 |
| Elite Tarmac Surfacing Ltd | Construction | 2019 |
| Elixir Financial Management Ltd | Financial services | 2010 |
| Elizabeth Cleaning Company Limited | Extraterritorial | 1986 |
| Ella Complex Care Limited | Health & social care | 2024 |