| European Smooth Alliance Ltd | Arts & recreation | 2025 |
| Europlan Associates Limited | Information & communication | 1997 |
| EV Chamberlain Ltd | Education | 2009 |
| Evai Ratings & Research Limited | Professional & scientific | 2019 |
| Evaleys Ltd | Real estate | 2026 |
| EVC Digital Limited | Arts & recreation | 2002 |
| Everlast Improvements Ltd | Construction | 2014 |
| Eversfield Court Tenants Association Limited | Real estate | 2004 |
| Eversfield House Residents' Association Limited | Households | 1974 |
| Eversfield View Ltd | Information & communication | 2021 |
| Everso Creative Limited | Professional & scientific | 2000 |
| Everwell Nutrition Limited | Health & social care | 2025 |
| Everyprint Limited | Professional & scientific | 2005 |
| Everything Trading Limited | Wholesale & retail | 2020 |
| Evol Empire Limited | Financial services | 2019 |
| Evolution Cycles Ltd | Wholesale & retail | 2008 |
| Evolving Places Limited | Construction | 2015 |
| EX730 Limited | Manufacturing | 2026 |
| Exams Platform Ltd | Education | 2021 |
| Exceat Enterprises Limited | Financial services | 2022 |
| Excellentinall Ltd | Administrative & support | 2025 |
| Exclusive Kitchens Wimbledon Ltd | Wholesale & retail | 2011 |
| Exclusively Holiday Homes Limited | Hospitality | 2012 |
| Expatro Ltd | Administrative & support | 2025 |
| Experium Financial Services Ltd | Extraterritorial | 2014 |
| Expert Edge Property Limited | Real estate | 2024 |
| Exploring Comfort Ltd | Administrative & support | 2025 |
| Exposure Automotive Limited | Wholesale & retail | 2012 |
| Exposure Electronics Limited | Manufacturing | 1996 |
| Express & Global Limited | Transport & logistics | 2002 |
| Express Charters Limited | Extraterritorial | 2013 |
| Express Recruitment and Training Ltd | Administrative & support | 2022 |
| Extinol Limited | Manufacturing | 2011 |
| Extraverte Community Projects CIC | Professional & scientific | 2011 |
| EYE Surgeon London Limited | Health & social care | 2018 |
| Eyes Open 11 Ltd | Wholesale & retail | 2019 |
| Eythorne Village Store Limited | Wholesale & retail | 2012 |
| F & C Cleaning Services Ltd | Administrative & support | 2018 |
| F & L Plumbing and Heating Services Limited | Construction | 2020 |
| F & O Services Ltd | Construction | 2016 |
| F E Page Limited | Administrative & support | 2012 |
| F&L Property and Building Solutions Ltd | Construction | 2024 |
| F&Z Property Ltd | Real estate | 2021 |
| FAB Gelato Ltd | Hospitality | 2025 |
| Fairfield Lodge Limited | Extraterritorial | 2011 |
| Fairfield Lodge RTM Company Limited | Extraterritorial | 2008 |
| Fairgirth Ltd | Professional & scientific | 2024 |
| Fairlight Homes (UK) Ltd | Construction | 2016 |
| Faith Care Agency Limited | Other services | 2015 |
| Faith Healthcare Solutions Limited | Other services | 2015 |
| Fake N Real Installations Ltd | Professional & scientific | 2020 |
| Falafel Box Limited | Wholesale & retail | 2023 |
| Farm House Pizza (Eastbourne) Ltd | Hospitality | 2018 |
| Farmcare World Limited | Wholesale & retail | 2016 |
| Farmers' Produce & Craft Market (Hailsham) Limited | Agriculture & fishing | 1999 |
| Farrington Court (Eastbourne) Limited | Households | 1993 |
| Fasn Accountancy & Business Solutions Ltd | Professional & scientific | 2014 |
| Fast Fixings Limited | Administrative & support | 2003 |
| Fast Logistcs Ltd | Transport & logistics | 2025 |
| Fast Train Media Limited | Information & communication | 2022 |
| Fastgrid Ltd | Construction | 2026 |
| Fasthaven Limited | Wholesale & retail | 1985 |
| Fastsole Private Ltd | Other services | 2017 |
| FAT JOR Ltd. | Manufacturing | 2021 |
| Fazli Traders Ltd | Wholesale & retail | 2025 |
| FB Academy Limited | Education | 2024 |
| FC Imaging Ltd | Health & social care | 2024 |
| Fcrc & SP Angels Ltd | Other services | 2021 |
| FD & Co UK Limited | Real estate | 2011 |
| Fdoc Consulting Limited | Professional & scientific | 2024 |
| Feastival Limited | Arts & recreation | 2023 |
| Feature Architectural Fabrications Limited | Manufacturing | 2023 |
| Features Beauty Sussex Ltd. | Other services | 2021 |
| Feederland Limited | Wholesale & retail | 2017 |
| Feetclinic Limited | Extraterritorial | 2015 |
| Fefe Limited | Wholesale & retail | 2024 |
| Felix.Z Ltd | Wholesale & retail | 2025 |
| Felstead Commercial Ltd | Wholesale & retail | 2020 |
| Felt Studios Limited | Manufacturing | 2003 |
| Feltham Dental Limited | Health & social care | 2020 |
| Femy's Oriental Limited | Wholesale & retail | 2024 |
| FEN Farm Property Limited | Administrative & support | 2014 |
| Fenton Gardens Ltd | Construction | 2019 |
| Fern Clothing Limited | Wholesale & retail | 2025 |
| Fernandes Cafe Limited | Hospitality | 2025 |
| Ferring Dental Clinic Limited | Health & social care | 2011 |
| Ferris Plumbing & Heating Ltd | Construction | 2015 |
| Fgarcia.Co.Uk Ltd | Information & communication | 2007 |
| FHC London Ltd | Real estate | 2019 |
| Fibremark Solutions Limited | Professional & scientific | 2015 |
| Fiducia Marketing Solutions Limited | Administrative & support | 2014 |
| Field Green Barn Limited | Real estate | 2023 |
| Field of Vision Productions Limited | Administrative & support | 2011 |
| Field Plant Hire Limited | Administrative & support | 2016 |
| Fieldings (Eastbourne) Management Company Limited(The) | Households | 1977 |
| Fieldskill Investments Ltd | Professional & scientific | 2014 |
| Fieri Leadership and Development Experts LLP | | 2012 |
| Fieri Leadership and Development Ltd | Professional & scientific | 2021 |
| Fiftysix Sales Ltd | Administrative & support | 2017 |
| Figo & Fago Limited | Wholesale & retail | 2023 |